DOI: 10.1242/dev.124.11.2235. The mesenchymal factor, FGF10, initiates and maintains the outgrowth of the chick limb bud through interaction with FGF8, an apical ectodermal factor

The mesenchymal factor, FGF10, initiates and maintains the outgrowth of the chick limb bud through interaction with FGF8, an apical ectodermal factor

10.1242/dev.124.11.2235

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

ABSTRACT Vertebrate limb formation has been known to be initiated by a factor(s) secreted from the lateral plate mesoderm. In this report, we provide evidence that a member of the fibroblast growth factor (FGF) family, FGF10, emanates from the prospective limb mesoderm to serve as an endogenous initiator for limb bud formation. Fgf10 expression in the prospective limb mesenchyme precedes Fgf8 expression in the nascent apical ectoderm. Ectopic application of FGF10 to the chick embryonic flank can induce Fgf8 expression in the adjacent ectoderm, resulting in the formation of an additional complete limb. Expression of Fgf10 persists in the mesenchyme of the established limb bud and appears to interact with Fgf8 in the apical ectoderm and Sonic hedgehog in the zone of polarizing activity. These results suggest that FGF10 is a key mesenchymal factor involved in the initial budding as well as the continuous outgrowth of vertebrate limbs.

Bibliography

Ohuchi, H., Nakagawa, T., Yamamoto, A., Araga, A., Ohata, T., Ishimaru, Y., Yoshioka, H., Kuwana, T., Nohno, T., Yamasaki, M., Itoh, N., & Noji, S. (1997). The mesenchymal factor, FGF10, initiates and maintains the outgrowth of the chick limb bud through interaction with FGF8, an apical ectodermal factor. Development, 124(11), 2235â2244.

Authors 12

Hideyo Ohuchi (first)
Takashi Nakagawa (additional)
Atsuyo Yamamoto (additional)
Akihiro Araga (additional)
Takeshi Ohata (additional)
Yoshiyasu Ishimaru (additional)
Hidefumi Yoshioka (additional)
Takashi Kuwana (additional)
Tsutomu Nohno (additional)
Masahiro Yamasaki (additional)
Nobuyuki Itoh (additional)
Sumihare Noji (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 10:46 p.m.)
Deposited	6 months, 3 weeks ago (Feb. 13, 2025, 10:17 p.m.)
Indexed	1 week, 2 days ago (Aug. 26, 2025, 2:56 a.m.)
Issued	28 years, 3 months ago (June 1, 1997)
Published	28 years, 3 months ago (June 1, 1997)
Published Online	28 years, 3 months ago (June 1, 1997)
Published Print	28 years, 3 months ago (June 1, 1997)

Funders 0

None

BibTeX

@article{Ohuchi_1997, title={The mesenchymal factor, FGF10, initiates and maintains the outgrowth of the chick limb bud through interaction with FGF8, an apical ectodermal factor}, volume={124}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.124.11.2235}, DOI={10.1242/dev.124.11.2235}, number={11}, journal={Development}, publisher={The Company of Biologists}, author={Ohuchi, Hideyo and Nakagawa, Takashi and Yamamoto, Atsuyo and Araga, Akihiro and Ohata, Takeshi and Ishimaru, Yoshiyasu and Yoshioka, Hidefumi and Kuwana, Takashi and Nohno, Tsutomu and Yamasaki, Masahiro and Itoh, Nobuyuki and Noji, Sumihare}, year={1997}, month=jun, pages={2235–2244} }

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  "abstract": "<jats:title>ABSTRACT</jats:title>\n               <jats:p>Vertebrate limb formation has been known to be initiated by a factor(s) secreted from the lateral plate mesoderm. In this report, we provide evidence that a member of the fibroblast growth factor (FGF) family, FGF10, emanates from the prospective limb mesoderm to serve as an endogenous initiator for limb bud formation. Fgf10 expression in the prospective limb mesenchyme precedes Fgf8 expression in the nascent apical ectoderm. Ectopic application of FGF10 to the chick embryonic flank can induce Fgf8 expression in the adjacent ectoderm, resulting in the formation of an additional complete limb. Expression of Fgf10 persists in the mesenchyme of the established limb bud and appears to interact with Fgf8 in the apical ectoderm and Sonic hedgehog in the zone of polarizing activity. These results suggest that FGF10 is a key mesenchymal factor involved in the initial budding as well as the continuous outgrowth of vertebrate limbs.</jats:p>",
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