DOI: 10.1242/dev.122.9.2921. The patched signaling pathway mediates repression of gooseberry allowing neuroblast specification by wingless during Drosophila neurogenesis

The patched signaling pathway mediates repression of gooseberry allowing neuroblast specification by wingless during Drosophila neurogenesis

10.1242/dev.122.9.2921

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

ABSTRACT The Drosophila signaling molecule Wingless (Wg) plays crucial roles in cell-cell communications during development. In the developing nervous system, a previous study has shown that Wg acts non-autonomously to specify the fate of a specific neuronal precursor, NB4-2 (Q. ChuLaGraff and C. Q. Doe (1993) Science 261, 1594-1597). The lack of autocrine specification of NB4-2 in Wg-expressing cells suggests that the response to Wg is spatially restricted, presumably through the activity of the Wg-receptor. I show that two other proteins, a transcription factor Gooseberry (Gsb) and a transmembrane protein Patched (Ptc), participate in the Wg-mediated specification of NB4-2 by controlling the response to the Wg signal. In gsb mutants, Wgpositive NB5-3 is transformed to NB4-2 in a Wg-dependent manner, suggesting that Gsb normally represses the capacity to respond to the Wg signal. In ptc mutants, Gsb is ectopically expressed in normally Wg-responsive cells, thus preventing the Wg response and consequently the correct specification of NB4-2 does not take place. This conclusion is supported by the observation that NB4-2 can be specified in gsb;ptc double mutants in a Wg-dependent manner. Moreover, ectopic expression of Gsb from the hsp70-gsb transgene also blocks the response to the Wg signal. I propose that the responsiveness to the Wg signal is controlled by sequential negative regulation, ptc→gsb→Wg receptor. The timing of the response to Gsb suggests that the specification of neuroblast identities takes place within the neuroectoderm, prior to neuroblast delamination.

Bibliography

Bhat, K. M. (1996). The patched signaling pathway mediates repression of gooseberry allowing neuroblast specification by wingless during Drosophila neurogenesis. Development, 122(9), 2921â2932.

Authors 1

Krishna Moorthi Bhat (first)

References 34 Referenced 53

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10.1242/dev.119.2.501 / Development / Segment polarity gene interactions modulate epidermal patterning in Drosophila embryos by Bejsovec (1993)
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10.1128/MCB.15.8.4052 / Mol. Cell. Biol / mitimere and pdm1 genes collaborate during specification of the RP2/sib lineage in Drosophila neurogenesis by Bhat (1995)
10.1242/dev.122.4.1113 / Development, in press / The GAGA factor is required in the early Drosophila embryo not only for transcriptional regulation but also for nuclear division by Bhat (1996)
10.1016/0092-8674(86)90818-4 / Cell / Conservation of a large protein domain in the segmentation gene paired and functionally related genes of Drosophila by Bopp (1986)
10.1242/dev.115.4.893 / Development / The generation of cell diversity during early neurogenesis in Drosophila by Cabrera (1992)
{'key': '2025021323115445700_DEVELOP_122_9_2921C9', 'first-page': '1091', 'volume-title': 'The Development of Drosophila melanogaster', 'author': 'Campos-Ortega', 'year': '1993'} / The Development of Drosophila melanogaster by Campos-Ortega (1993)
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Dates

Type	When
Created	4 years, 4 months ago (April 23, 2021, 3:03 p.m.)
Deposited	6 months, 2 weeks ago (Feb. 13, 2025, 6:12 p.m.)
Indexed	1 month, 2 weeks ago (July 14, 2025, 11:24 p.m.)
Issued	29 years ago (Sept. 1, 1996)
Published	29 years ago (Sept. 1, 1996)
Published Online	29 years ago (Sept. 1, 1996)
Published Print	29 years ago (Sept. 1, 1996)

Funders 0

None

BibTeX

@article{Bhat_1996, title={The patched signaling pathway mediates repression of gooseberry allowing neuroblast specification by wingless during Drosophila neurogenesis}, volume={122}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.122.9.2921}, DOI={10.1242/dev.122.9.2921}, number={9}, journal={Development}, publisher={The Company of Biologists}, author={Bhat, Krishna Moorthi}, year={1996}, month=sep, pages={2921–2932} }

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