DOI: 10.1242/dev.122.7.2023. Basic fibroblast growth factor induces notochord formation and the expression of <i>As-T</i>, a <i>Brachyury</i> homolog, during ascidian embryogenesis

Basic fibroblast growth factor induces notochord formation and the expression of As-T, a Brachyury homolog, during ascidian embryogenesis

10.1242/dev.122.7.2023

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

ABSTRACT The tadpole larva of an ascidian develops 40 notochord cells in the center of its tail. Most of the notochord cells originate from the A-line precursors, among which inductive interactions are required for the subsequent differentiation of notochord. The presumptive-endoderm blastomeres or presumptive-notochord blastomeres themselves are inducers of notochord formation. Notochord induction takes place during the 32-cell stage. In amphibia, mesoderm induction is thought to be mediated by several growth factors, for example, activins and basic fibroblast growth factor (bFGF). In the ascidian, Halocynthia roretzi, treatment with bFGF of presumptive-notochord blastomeres that had been isolated at the early 32-cell stage promoted the formation of notochord at a low concentration of bFGF (0.02 ng/ml), while activin failed to induce notochord differentiation. The effect of bFGF reached a maximum at the end of the 32-cell stage and rapidly faded at the beginning of the subsequent cleavage, the time for full induction of notochord being at least 20 minutes. The expression of As-T, a previously isolated ascidian homolog of the mouse Brachyury (T) gene, starts at the 64cell stage and is detectable exclusively in the presumptivenotochord blastomeres. The present study showed that presumptive-notochord blastomeres, isolated at the early 32-cell stage, neither differentiated into notochord nor expressed the As-T gene. However, when the presumptivenotochord blastomeres were coisolated or recombined with inducer blastomeres, transcripts of As-T were detected. When presumptive-notochord blastomeres were treated with bFGF, the expression of the As-T gene was also detected. These results suggest that inductive interaction is required for the expression of the As-T gene and that the expression of the As-T gene is closely correlated with the determined state of the notochord-precursor cells.

Bibliography

Nakatani, Y., Yasuo, H., Satoh, N., & Nishida, H. (1996). Basic fibroblast growth factor induces notochord formation and the expression of As-T, a Brachyury homolog, during ascidian embryogenesis. Development, 122(7), 2023â2031.

Authors 4

Yuki Nakatani (first)
Hitoyoshi Yasuo (additional)
Noriyuki Satoh (additional)
Hiroki Nishida (additional)

References 30 Referenced 90

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{'key': '2025021323035903900_DEVELOP_122_7_2023C2', 'first-page': '1', 'article-title': 'The organization and cell-lineage of the ascidian egg', 'volume': '13', 'author': 'Conklin', 'year': '1905', 'journal-title': 'J. Acad. Nat. Sci. (Philadelphia)'} / J. Acad. Nat. Sci. (Philadelphia) / The organization and cell-lineage of the ascidian egg by Conklin (1905)
10.1038/347391a0 / Nature / Graded changes in dose of a Xenopus activin A homologue elicit stepwise transitions in embryonic cell fate by Green (1990)
10.1242/dev.108.1.173 / Development / The biological effects of XTC-MIF: quantitative comparison with Xenopus bFGF by Green (1990)
10.1038/343617a0 / Nature / Cloning of theT gene required in mesoderm formation in the mouse by Herrmann (1990)
10.1242/dev.114.3.711 / Development / Expression of a novel FGF in the Xenopus embryo. A new candidate inducing factor for mesoderm formation and anteroposterior specification by Issacs (1992)
10.1002/j.1460-2075.1994.tb06769.x / EMBO J / eFGF regulates Xbra expression during Xenopus gastrulation by Issacs (1994)
10.1016/0092-8674(87)90110-3 / Cell / Synergistic induction of mesoderm by FGF and TGF-ß and the identification of an mRNA coding for FGF in the early Xenopus embryo by Kimelman (1987)
10.1242/dev.114.1.261 / Development / Induction of dorsal and ventral mesoderm by ectopically expressed Xenopus basic fibroblast growth factor by Kimelman (1992)
10.1006/dbio.1995.1090 / Dev. Biol / The chick Brachyury gene: developmental expression pattern and response to axial induction by localized activin by Kispert (1995)
10.1111/j.1440-169X.1989.00071.x / Dev. Growth Differ / Temporal expression of myosin heavy chain gene during ascidian embryogenesis by Makabe (1989)
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Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 10:39 p.m.)
Deposited	6 months, 2 weeks ago (Feb. 13, 2025, 6:04 p.m.)
Indexed	6 months, 2 weeks ago (Feb. 14, 2025, 12:32 a.m.)
Issued	29 years, 2 months ago (July 1, 1996)
Published	29 years, 2 months ago (July 1, 1996)
Published Online	29 years, 2 months ago (July 1, 1996)
Published Print	29 years, 2 months ago (July 1, 1996)

Funders 0

None

BibTeX

@article{Nakatani_1996, title={Basic fibroblast growth factor induces notochord formation and the expression of As-T, a Brachyury homolog, during ascidian embryogenesis}, volume={122}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.122.7.2023}, DOI={10.1242/dev.122.7.2023}, number={7}, journal={Development}, publisher={The Company of Biologists}, author={Nakatani, Yuki and Yasuo, Hitoyoshi and Satoh, Noriyuki and Nishida, Hiroki}, year={1996}, month=jul, pages={2023–2031} }

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