DOI: 10.1242/dev.121.9.2983. Analysis of FGF function in normal and <i>no tail</i> zebrafish embryos reveals separate mechanisms for formation of the trunk and the tail

Analysis of FGF function in normal and no tail zebrafish embryos reveals separate mechanisms for formation of the trunk and the tail

10.1242/dev.121.9.2983

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

ABSTRACT To analyse the roles of FGF activity and brachyury during gastrulation we have directly compared the consequences of inhibition of FGF-receptor signalling with the phenotype of the zebrafish brachyury mutant, no tail (ntl). We show that expression of ntl is regulated by FGF and that inhibition of FGF receptor-signalling leads to complete loss of the trunk and tail. Since the ntl mutant lacks the tail and notochord but has an otherwise normal trunk, this demonstrates that trunk development is dependent upon an unidentified gene, or set of genes, referred to as no trunk (ntk) which is regulated by FGF. We propose a model to explain the FGF-dependent regulation of ntl and ntk that accounts for the above phenotypes. Consistent with this model, over-expression of eFGF led to suppression of anterior fates and development of trunk and tail derivatives only. In addition, widespread activation of convergence and extension movements resulted in the formation of multiple axis-like structures. Expression of eve1 and cad1 was also regulated by FGF activity, suggesting that during gastrulation FGF activity is normally restricted to the germ ring where these genes, and ntl, are expressed. Taken together these data suggest that the germ ring acts as a posteriorising centre during AP patterning, mediated by FGF activity in this tissue.

Bibliography

Griffin, K., Patient, R., & Holder, N. (1995). Analysis of FGF function in normal and no tail zebrafish embryos reveals separate mechanisms for formation of the trunk and the tail. Development, 121(9), 2983â2994.

Authors 3

Kevin Griffin, (first)
Roger Patient (additional)
Nigel Holder (additional)

References 48 Referenced 193

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Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 10:27 p.m.)
Deposited	7 months, 2 weeks ago (Jan. 15, 2025, 10:03 a.m.)
Indexed	4 months, 3 weeks ago (April 7, 2025, 5:49 p.m.)
Issued	29 years, 11 months ago (Sept. 1, 1995)
Published	29 years, 11 months ago (Sept. 1, 1995)
Published Online	29 years, 11 months ago (Sept. 1, 1995)
Published Print	29 years, 11 months ago (Sept. 1, 1995)

Funders 0

None

BibTeX

@article{Griffin__1995,_Patient_Holder_1995, title={Analysis of FGF function in normal and no tail zebrafish embryos reveals separate mechanisms for formation of the trunk and the tail}, volume={121}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.121.9.2983}, DOI={10.1242/dev.121.9.2983}, number={9}, journal={Development}, publisher={The Company of Biologists}, author={Griffin, Kevin and Patient, Roger and Holder, Nigel}, year={1995}, month=sep, pages={2983–2994} }

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