DOI: 10.1242/dev.121.10.3215. Micromeres are required for normal vegetal plate specification in sea urchin embryos

Micromeres are required for normal vegetal plate specification in sea urchin embryos

10.1242/dev.121.10.3215

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

ABSTRACT Vegetal plate specification was assessed in S. purpuratus embryos after micromere deletions at the 4th, 5th and 6th cleavages, by assaying expression of the early vegetal plate marker Endo16, using whole-mount in situ hybridization. After 4th cleavage micromere deletions, the embryos typically displayed weak Endo16 expression in relatively few cells of the lineages that normally constitute the vegetal plate, while after 5th and 6th cleavage micromere deletions the embryos exhibited strong Endo16 expression in larger fractions of cells belonging to those lineages. When all four micromeres were deleted, the embryos were severely delayed in initiating gastrulation and sometimes failed to complete gastrulation. However, if only one micromere was allowed to remain in situ throughout development, the embryos exhibited strong Endo16 expression and gastrulation occurred normally, on schedule with controls. Additional measurements showed that these microsurgical manipulations do not alter cleavage rates or generally disrupt embryo organization. These results constitute direct evidence that the micromeres provide signals required by the macromere lineages for initiation of vegetal plate specification. The specification of the vegetal plate is completed in a normal manner only if micromere signaling is allowed to continue at least to the 6th cleavage stage.

Bibliography

Ransick, A., & Davidson, E. H. (1995). Micromeres are required for normal vegetal plate specification in sea urchin embryos. Development, 121(10), 3215â3222.

Authors 2

Andrew Ransick (first)
Eric H. Davidson (additional)

References 14 Referenced 123

10.1101/gad.1.1.75 / Genes Dev / Lineage and fate of each blastomere of the eight-cell sea urchin embryo by Cameron (1987)
10.1242/dev.113.4.1085 / Development / Macromere cell fates during sea urchin development by Cameron (1991)
{'volume-title': 'Gene Activity in Early Development', 'year': '1986', 'author': 'Davidson', 'key': '2025011515043503500_DEVELOP_121_10_3215C3'} / Gene Activity in Early Development by Davidson (1986)
10.1242/dev.105.3.421 / Development / Lineage-specific gene expression and the regulative capacities of the sea urchin embryo: A proposed mechanism by Davidson (1989)
10.1242/dev.108.3.365 / Development / How embryos work: A comparative view of diverse modes of cell fate specification by Davidson (1990)
10.1242/dev.113.1.1 / Development / Spatial mechanisms of gene regulation in metazoan embryos by Davidson (1991)
10.1016/0012-1606(85)90376-8 / Dev. Biol / Three cell recognition changes accompany the ingression of sea urchin primary mesenchyme cells by Fink (1985)
{'key': '2025011515043503500_DEVELOP_121_10_3215C8', 'first-page': '251', 'article-title': 'Uber die Determination im Verlaufe der Eiachse bei Seeigeln', 'volume': '14', 'author': 'Hörstadius', 'year': '1935', 'journal-title': 'Pubb. Staz. Zool. Napoli'} / Pubb. Staz. Zool. Napoli / Uber die Determination im Verlaufe der Eiachse bei Seeigeln by Hörstadius (1935)
10.1111/j.1469-185X.1939.tb00929.x / Biol. Rev. Cambridge Phil. Soc / The mechanics of sea urchin development studied by operative methods by Hörstadius (1939)
10.1242/dev.116.Supplement.33 / Development / Pattern formation during gastrulation in the sea urchin embryo by McClay (1992)
10.1016/0012-1606(89)90147-4 / Dev. Biol / Endo16, a lineage-specific protein of the sea urchin embryo, is first expressed just prior to gastrulation by Nocente-McGrath (1989)
10.1126/science.8438164 / Science / A complete second gut induced by transplanted micromeres in the sea urchin embryo by Ransick (1993)
10.1016/0925-4773(93)90001-E / Mech. Dev / Whole mount in situ hybridization shows Endo16 to be a marker for the vegetal plate territory in sea urchin embryos by Ransick (1993)
10.1006/dbio.1994.1235 / Dev. Biol / Endo16, a large multidomain protein found on the surface and ECM of endodermal cells during sea urchin gastrulation, binds calcium by Soltysik-Española (1994)

Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 10:11 p.m.)
Deposited	7 months, 3 weeks ago (Jan. 15, 2025, 10:05 a.m.)
Indexed	1 month ago (July 30, 2025, 10:08 a.m.)
Issued	29 years, 11 months ago (Oct. 1, 1995)
Published	29 years, 11 months ago (Oct. 1, 1995)
Published Online	29 years, 11 months ago (Oct. 1, 1995)
Published Print	29 years, 11 months ago (Oct. 1, 1995)

Funders 0

None

BibTeX

@article{Ransick_1995, title={Micromeres are required for normal vegetal plate specification in sea urchin embryos}, volume={121}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.121.10.3215}, DOI={10.1242/dev.121.10.3215}, number={10}, journal={Development}, publisher={The Company of Biologists}, author={Ransick, Andrew and Davidson, Eric H.}, year={1995}, month=oct, pages={3215–3222} }

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