DOI: 10.1242/dev.120.7.1767. Neural selective activation and temporal regulation of a mammalian GAP-43 promoter in zebrafish

Neural selective activation and temporal regulation of a mammalian GAP-43 promoter in zebrafish

10.1242/dev.120.7.1767

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

ABSTRACT Neurons throughout the vertebrate nervous system selectively activate the gene for a growth cone component, GAP-43, during embryonic development, and then decrease its expression abruptly as they form synapses. Distal interruption of mature axons in the central nervous system (CNS) of fish and amphibians, but not in the mammalian CNS reverses the developmental down-regulation of GAP-43 expression. To explore functional conservation and divergence of cis-acting elements that regulate expression of the GAP-43 gene, we studied activation, in transgenic zebrafish embryos, of mammalian GAP-43 genomic sequences fused to a marker gene. The DNA fragments containing the GAP-43 promoter, including a short fragment of 386 base pairs, were preferentially activated in the embryonic fish nervous system at times when extensive neuronal differentiation and neurite outgrowth take place. After 2 days of development, expression of the mammalian transgenes was specifically downregulated in the fish spinal cord but increased in more rostral regions of the CNS. This expression pattern was well correlated with the regulation of the endogenous fish GAP-43 gene revealed by in situ hybridization. Elements of the mammalian gene located a substantial distance upstream of the minimal promoter directed additional expression of the marker gene in a specific set of non-neural cells in zebrafish embryos. Our results indicate that cis-acting elements of the GAP-43 gene, and signaling pathways controlling these elements during embryonic development, have been functionally conserved in vertebrate evolution.

Bibliography

Reinhard, E., Nedivi, E., Wegner, J., Skene, J. H. P., & Westerfield, M. (1994). Neural selective activation and temporal regulation of a mammalian GAP-43 promoter in zebrafish. Development, 120(7), 1767â1775.

Authors 5

Eva Reinhard (first)
Elly Nedivi (additional)
Jeremy Wegner (additional)
J. H. Pate Skene (additional)
Monte Westerfield (additional)

References 45 Referenced 62

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{'key': '2024121723404003300_DEVELOP_120_7_1767C6', 'article-title': 'B-50/GAP-43 expression correlates with process outgrowth in the embryonic mouse nervous system', 'volume-title': 'Eur. J. Neurosci. 2, 487-499', 'author': 'Biffo', 'year': '1990'} / Eur. J. Neurosci. 2, 487-499 / B-50/GAP-43 expression correlates with process outgrowth in the embryonic mouse nervous system by Biffo (1990)
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Dates

Type	When
Created	4 years, 4 months ago (April 23, 2021, 2:54 p.m.)
Deposited	8 months, 2 weeks ago (Dec. 17, 2024, 6:40 p.m.)
Indexed	3 weeks, 5 days ago (Aug. 6, 2025, 8:56 a.m.)
Issued	31 years, 2 months ago (July 1, 1994)
Published	31 years, 2 months ago (July 1, 1994)
Published Online	31 years, 2 months ago (July 1, 1994)
Published Print	31 years, 2 months ago (July 1, 1994)

Funders 0

None

BibTeX

@article{Reinhard_1994, title={Neural selective activation and temporal regulation of a mammalian GAP-43 promoter in zebrafish}, volume={120}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.120.7.1767}, DOI={10.1242/dev.120.7.1767}, number={7}, journal={Development}, publisher={The Company of Biologists}, author={Reinhard, Eva and Nedivi, Elly and Wegner, Jeremy and Skene, J. H. Pate and Westerfield, Monte}, year={1994}, month=jul, pages={1767–1775} }

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