DOI: 10.1242/dev.120.10.3033. <i>bcl-xLx</i> is the major <i>bcl-x</i> mRNA form expressed during murine development and its product localizes to mitochondria

bcl-xLx is the major bcl-x mRNA form expressed during murine development and its product localizes to mitochondria

10.1242/dev.120.10.3033

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

ABSTRACT Most examples of cell death in animals are controlled by a genetic program that is activated within the dying cell. The apoptotic process is further regulated by a set of genes that act as repressors of cell death. Of these, bcl-2 is expressed in a variety of embryonic and postnatal tissues which suggests a critical role for bcl-2 in organogenesis and tissue homeostasis. Surprisingly, mutant mice with targeted disruption of bcl-2 appear normal at birth and complete maturation of lymphoid tissues before succumbing to fulminant lymphopenia and polycystic renal disease by 2-5 weeks of age. This suggests that there may be genes other than bcl-2 that can regulate apoptosis during development. To begin to investigate this possibility, we have cloned and characterized the murine bcl-x gene, whose human counterpart displays striking homology to bcl-2. The predicted murine bcl-xL gene product exhibits a high level of amino acid identity (97%) to its human counterpart. Just like Bcl-2, the murine bcl-xL gene product can act as a dominant inhibitor of cell death upon growth factor withdrawal. In addition, the bulk of the bcl-xL product localizes to the periphery of mitochondria as assessed by a bcl-xL-tag expression system, suggesting that both Bcl-2 and Bcl-xL proteins prevent cell death by a similar mechanism. bcl-xL is the most abundant bcl-x mRNA species expressed in embryonic and adult tissues. The levels of bcl-xL mRNA appear higher than those of bcl-2 during embryonal development and in several adult organs including bone marrow, brain, kidney and thymus. In addition to bcl-xL, we have identified another form of bcl-x mRNA, bcl-xβ, that results from an unspliced bcl-x transcript. bcl-xβ mRNA is expressed in various embryonic and postnatal tissues. Surprisingly, the expression of bcl-xS (a negative regulator of programmed cell death) was undetectable by a sensitive S1-nuclease assay and polymerase chain reaction analysis of mouse tissues. Based on its tissue and developmental patterns of expression, it appears that bcl-x may play an important role in the regulation of cell death during development and tissue homeostasis.

Bibliography

GonzÃ¡lez-GarcÃa, M., PÃ©rez-Ballestero, R., Ding, L., Duan, L., Boise, L. H., Thompson, C. B., & NÃºÃ±ez, G. (1994). bcl-xLx is the major bcl-x mRNA form expressed during murine development and its product localizes to mitochondria. Development, 120(10), 3033â3042.

Authors 7

Maribel González-García (first)
Rafael Pérez-Ballestero (additional)
Liyun Ding (additional)
Linda Duan (additional)
Lawrence H. Boise (additional)
Craig B. Thompson (additional)
Gabriel Núñez (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 9:33 p.m.)
Deposited	8 months, 2 weeks ago (Dec. 17, 2024, 6:47 p.m.)
Indexed	51 minutes ago (Sept. 2, 2025, 9:54 p.m.)
Issued	30 years, 11 months ago (Oct. 1, 1994)
Published	30 years, 11 months ago (Oct. 1, 1994)
Published Online	30 years, 11 months ago (Oct. 1, 1994)
Published Print	30 years, 11 months ago (Oct. 1, 1994)

Funders 0

None

BibTeX

@article{Gonz_lez_Garc_a_1994, title={bcl-xLx is the major bcl-x mRNA form expressed during murine development and its product localizes to mitochondria}, volume={120}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.120.10.3033}, DOI={10.1242/dev.120.10.3033}, number={10}, journal={Development}, publisher={The Company of Biologists}, author={González-García, Maribel and Pérez-Ballestero, Rafael and Ding, Liyun and Duan, Linda and Boise, Lawrence H. and Thompson, Craig B. and Núñez, Gabriel}, year={1994}, month=oct, pages={3033–3042} }

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