DOI: 10.1242/dev.120.10.2901. GLP-1 is localized to the mitotic region of the <i>C. elegans</i> germ line

GLP-1 is localized to the mitotic region of the C. elegans germ line

10.1242/dev.120.10.2901

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

ABSTRACT In C. elegans, germline mitosis depends on induction by the somatic distal tip cell (DTC) and on activity of the glp-1 gene. Using antibodies to GLP-1 protein, we have examined GLP-1 on western blots and by immunocytochemistry. GLP-1 is tightly associated with membranes of mitotic germline cells, supporting its identification as an integral membrane protein. Furthermore, GLP-1 is localized within the germ line to the mitotic region, consistent with the model that GLP-1 acts as a membrane receptor for the distal tip cell signal. Unexpectedly, GLP-1 and the zone of mitosis extend further than the DTC processes. We present three models by which the DTC may influence GLP-1 activity and thereby determine the zone of mitosis. The spatial restriction of GLP-1 appears to be controlled at the translational level in hermaphrodites. We suggest that down-regulation of GLP-1 may be required to effect the transition from mitosis into meiosis.

Bibliography

Crittenden, S. L., Troemel, E. R., Evans, T. C., & Kimble, J. (1994). GLP-1 is localized to the mitotic region of the C. elegans germ line. Development, 120(10), 2901â2911.

Authors 4

Sarah L. Crittenden (first)
Emily R. Troemel (additional)
Thomas C. Evans (additional)
Judith Kimble (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 9:32 p.m.)
Deposited	8 months, 1 week ago (Dec. 17, 2024, 6:42 p.m.)
Indexed	6 days, 17 hours ago (Aug. 19, 2025, 5:56 a.m.)
Issued	30 years, 10 months ago (Oct. 1, 1994)
Published	30 years, 10 months ago (Oct. 1, 1994)
Published Online	30 years, 10 months ago (Oct. 1, 1994)
Published Print	30 years, 10 months ago (Oct. 1, 1994)

Funders 0

None

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  "abstract": "<jats:title>ABSTRACT</jats:title>\n               <jats:p>In C. elegans, germline mitosis depends on induction by the somatic distal tip cell (DTC) and on activity of the glp-1 gene. Using antibodies to GLP-1 protein, we have examined GLP-1 on western blots and by immunocytochemistry. GLP-1 is tightly associated with membranes of mitotic germline cells, supporting its identification as an integral membrane protein. Furthermore, GLP-1 is localized within the germ line to the mitotic region, consistent with the model that GLP-1 acts as a membrane receptor for the distal tip cell signal. Unexpectedly, GLP-1 and the zone of mitosis extend further than the DTC processes. We present three models by which the DTC may influence GLP-1 activity and thereby determine the zone of mitosis. The spatial restriction of GLP-1 appears to be controlled at the translational level in hermaphrodites. We suggest that down-regulation of GLP-1 may be required to effect the transition from mitosis into meiosis.</jats:p>",
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