DOI: 10.1242/dev.120.1.155. Cytoplasmic calcium and <i>Fucus</i> egg activation

Cytoplasmic calcium and Fucus egg activation

10.1242/dev.120.1.155

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

ABSTRACT Eggs of the marine brown alga, Fucus serratus, exhibit small transient elevations of cytosolic Ca2+ of variable magnitude, corresponding to the onset of the fertilization potential. Microinjection of Ca2+ buffers (BAPTA (1-2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid)) at concentrations sufficient to block any global fertilization-associated Ca2+cyt elevation did not inhibit egg activation (monitored as exocytosis of cell wall) or subsequent development. However, egg activation could be inhibited with higher buffer concentrations. Br2BAPTA (Kd = 1.6 μM) was a more effective inhibitor of egg activation than BAPTA (Kd = 0.17 μM). Localized microinjection of Ca2+ produced only localized cell wall exocytosis at the injection site. Eggs injected with Br2BAPTA at intracellular concentrations, which blocked egg activation, exhibited prolonged fertilization potentials. 45Ca2+ influx across the plasma membrane increased during fertilization. Our results show that a large transient global elevation of Ca2+cyt is not necessary for Fucus egg activation but rather a localized elevation to micromolar levels results, at least in part, from increased Ca2+ influx across the plasma membrane. This is needed for early fertilization events, including the generation of the fertilization potential and cell wall secretion.

Bibliography

Roberts, S. K., Gillot, I., & Brownlee, C. (1994). Cytoplasmic calcium and Fucus egg activation. Development, 120(1), 155â163.

Authors 3

S. K. Roberts (first)
I. Gillot (additional)
C. Brownlee (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 23, 2021, 2:29 p.m.)
Deposited	2 years, 1 month ago (July 20, 2023, 5:35 a.m.)
Indexed	1 year, 2 months ago (June 2, 2024, 8:50 p.m.)
Issued	31 years, 8 months ago (Jan. 1, 1994)
Published	31 years, 8 months ago (Jan. 1, 1994)
Published Online	31 years, 8 months ago (Jan. 1, 1994)
Published Print	31 years, 8 months ago (Jan. 1, 1994)

Funders 0

None

BibTeX

@article{Roberts_1994, title={Cytoplasmic calcium and Fucus egg activation}, volume={120}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.120.1.155}, DOI={10.1242/dev.120.1.155}, number={1}, journal={Development}, publisher={The Company of Biologists}, author={Roberts, S. K. and Gillot, I. and Brownlee, C.}, year={1994}, month=jan, pages={155–163} }

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  "abstract": "<jats:title>ABSTRACT</jats:title>\n               <jats:p>Eggs of the marine brown alga, Fucus serratus, exhibit small transient elevations of cytosolic Ca2+ of variable magnitude, corresponding to the onset of the fertilization potential. Microinjection of Ca2+ buffers (BAPTA (1-2-bis(o-aminophenoxy)ethane-N,N,N\u2032,N\u2032-tetraacetic acid)) at concentrations sufficient to block any global fertilization-associated Ca2+cyt elevation did not inhibit egg activation (monitored as exocytosis of cell wall) or subsequent development. However, egg activation could be inhibited with higher buffer concentrations. Br2BAPTA (Kd = 1.6 \u03bcM) was a more effective inhibitor of egg activation than BAPTA (Kd = 0.17 \u03bcM). Localized microinjection of Ca2+ produced only localized cell wall exocytosis at the injection site. Eggs injected with Br2BAPTA at intracellular concentrations, which blocked egg activation, exhibited prolonged fertilization potentials. 45Ca2+ influx across the plasma membrane increased during fertilization. Our results show that a large transient global elevation of Ca2+cyt is not necessary for Fucus egg activation but rather a localized elevation to micromolar levels results, at least in part, from increased Ca2+ influx across the plasma membrane. This is needed for early fertilization events, including the generation of the fertilization potential and cell wall secretion.</jats:p>",
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