DOI: 10.1242/dev.118.4.1169. A central role for microtubules in the differentiation of <i>Drosophila</i> oocytes

A central role for microtubules in the differentiation of Drosophila oocytes

10.1242/dev.118.4.1169

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

ABSTRACT Drosophila oocytes develop within cysts containing 16 cells that are interconnected by cytoplasmic bridges. Although the cysts are syncytial, the 16 cells differentiate to form a single oocyte and 15 nurse cells, and several mRNAs that are synthesized in the nurse cells accumulate specifically in the oocyte. To gain insight into the mechanisms that generate the cytoplasmic asymmetry within these cysts, we have examined cytoskeletal organization during oocyte differentiation. Shortly after formation of the 16 cell cysts, a prominent microtubule organizing center (MTOC) is established within the syncytial cytoplasm, and at the time the oocyte is determined, a single microtubule cytoskeleton connects the oocyte with the remaining 15 cells of each cyst. Recessive mutations at the Bicaudal-D (Bic-D) and egalitarian (egl) loci, which block oocyte differentiation, disrupt formation and maintenance of this polarized microtubule cytoskeleton. Microtubule assembly-inhibitors phenocopy these mutations, and prevent oocyte-specific accumulation of oskar, cyclin B and 65F mRNAs. We propose that formation of the polarized microtubule cytoskeleton is required for oocyte differentiation, and that this structure mediates the asymmetric accumulation of mRNAs within the syncytial cysts.

Bibliography

Theurkauf, W. E., Alberts, B. M., Jan, Y. N., & Jongens, T. A. (1993). A central role for microtubules in the differentiation of Drosophila oocytes. Development, 118(4), 1169â1180.

Authors 4

William E. Theurkauf (first)
Bruce M. Alberts (additional)
Yuh Nung Jan (additional)
Thomas A. Jongens (additional)

References 32 Referenced 233

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10.1242/dev.115.4.989 / Development / 3′ non-translated sequences in Drosophila cyclin B transcripts direct posterior pole accumulation late in oogenesis and peri-nuclear association in syncytial embryos by Dalby (1992)
10.1093/genetics/83.1.67 / Genetics / Synaptonemal complexes during pre-meiotic DNA synthesis in oocytes of Drosophilamelanogaster by Day (1976)
10.1007/978-3-642-96436-7 / Microtubules by Dustin (1978)
10.1016/0092-8674(91)90137-N / Cell / oskar organizes the germ plasm and directs localization of the posterior determinant nanos by Ephrussi (1991)
10.1242/dev.109.2.425 / Development / Localization of vasa, a component of Drosophila polar granules, in maternal-effect mutations that alter anteroposterior polarity by Hay (1990)
10.1016/0092-8674(91)90136-M / Cell / oskar mRNA is localized to the posterior pole of the Drosophila oocyte by Kim-Ha (1991)
10.1002/jmor.1051210106 / J. Morphol / The division and differentiation of Drosophila cystocytes by Koch (1967)
10.1007/BF00336421 / Z. Zellerforsch / Further studies on the ring canal system of the ovarian cystocytes of Drosophila melanogaster by Koch (1969)
10.1007/BF00206261 / Cell Tissue Res / Multiple effects of colchicine on oogenesis in Drosophila: Induced sterility and switch of potential oocyte to nurse-cell developmental pathway by Koch (1983)
10.1101/gad.4.6.905 / Genes Dev / Posterior localization of vasa protein correlates with, but is not sufficient for, pole cell development by Lasko (1990)
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Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 9:26 p.m.)
Deposited	8 months, 1 week ago (Dec. 17, 2024, 6:06 p.m.)
Indexed	1 week, 2 days ago (Aug. 21, 2025, 2:22 p.m.)
Issued	32 years ago (Aug. 1, 1993)
Published	32 years ago (Aug. 1, 1993)
Published Online	32 years ago (Aug. 1, 1993)
Published Print	32 years ago (Aug. 1, 1993)

Funders 0

None

BibTeX

@article{Theurkauf_1993, title={A central role for microtubules in the differentiation of Drosophila oocytes}, volume={118}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.118.4.1169}, DOI={10.1242/dev.118.4.1169}, number={4}, journal={Development}, publisher={The Company of Biologists}, author={Theurkauf, William E. and Alberts, Bruce M. and Jan, Yuh Nung and Jongens, Thomas A.}, year={1993}, month=aug, pages={1169–1180} }

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