DOI: 10.1242/dev.118.2.523. In vitro stalk cell differentiation in wild-type and ‘slugger’ mutants of <i>Dictyostelium discoideum</i>

In vitro stalk cell differentiation in wild-type and ‘slugger’ mutants of Dictyostelium discoideum

10.1242/dev.118.2.523

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

ABSTRACT In ‘slugger’ mutants of Dictyostelium discoideum, aggregates of cells remain for an abnormally long time in the migratory phase under conditions where wild-type aggregates form fruiting bodies. In the present work, we have examined the relationship between the defect in fruiting body formation in these mutants and their ability to form mature stalk cells. We dissociated anterior cells from slugs of the mutants and their parents and tested their ability to form stalk cells when incubated at low density in the presence of (1) the stalk cell morphogen Differentiation Inducing Factor-1 (DIF1) together with cyclic AMP, or (2) 8-Br-cAMP, which is believed to penetrate cell membrane and activate cAMPdependent protein kinase (PKA). Most of the mutants were markedly defective in forming stalk cells in response to DIF-1 plus cAMP, confirming a close relationship between fruiting body formation and stalk cell maturation. On the other hand, many of these same mutants formed stalk cells efficiently in response to 8-Br-cAMP. This supports evidence for an essential role of PKA in stalk cell maturation and fruiting body formation. It also indicates that many of the mutants owe their slugger phenotype to defects in functions required for optimal adenylyl cyclase activity.

Authors 2

Kei Inouye (first)
Julian Gross (additional)

References 21 Referenced 23

10.1016/0012-1606(88)90244-8 / Dev. Biol / Cyclic AMP is an inhibitor of stalk cell differentiation in Dictyostelium discoideum by Berks (1988)
10.1016/0092-8674(92)90225-2 / Cell / Culmination in Dictyostelium is regulated by the cAMP-dependent protein kinase by Harwood (1992)
10.1242/dev.104.4.669 / Development / Induction by acid load of the maturation of prestalk cells in Dictyostelium discoideum by Inouye (1988)
10.1242/dev.111.3.779 / Development / An analysis of culmination in Dictyostelium using prestalk and stalk-specific cell autonomous markers by Jermyn (1991)
10.1016/S0091-679X(08)61661-1 / Methods in Cell Biology / Cell differentiation in monolayers and the investigation of slime mold morphogenesis by Kay (1987)
10.1242/dev.105.4.753 / Development / Evidence that elevated intracellular cyclic AMP triggers spore maturation in Dictyostelium by Kay (1989)
10.1242/dev.104.1.121 / Development / Stalk cell formation in monolayers from isolated prestalk and prespore cells of Dictyostelium discoideum: evidence for two populations of prestalk cells by Kwong (1988)
10.1111/j.1440-169X.1988.00573.x / Develop. Growth and Differ / Dual effects of cAMP on the stability of prespore vesicles and 8-bromo cAMP-enhanced maturation of spore and stalk cells of Dictyostelium discoideum by Maeda (1988)
10.1073/pnas.89.22.10701 / Proc Natl. Acad. Sci. USA / DdPK3, which plays essential roles during Dictyostelium development, encodes the catalytic subunit of cAMP-dependent protein kinase by Mann (1992)
10.1038/328811a0 / Nature / Chemical structure of the morphogen differentiation inducing factor from Dictyostelium discoideum by Morris (1987)
10.1042/bj2490903 / Biochem. J / Structure elucidation of two differentiation inducing factors (DIF-2 and DIF-3) from the cellular slime mould Dictyostelium discoideum by Morris (1988)
{'key': '2024121723015098100_DEVELOP_118_2_523C12', 'first-page': '1639', 'article-title': 'Genetic analysis of the slug stage of Dictyostelium discoideum', 'volume': '128', 'author': 'Newell', 'year': '1982', 'journal-title': 'J. Gen. Microbiol'} / J. Gen. Microbiol / Genetic analysis of the slug stage of Dictyostelium discoideum by Newell (1982)
10.1016/0022-2836(77)90124-3 / J. Mol. Biol / Ammonia determines the choice of morphogenetic pathways in Dictyostelium discoideum by Schindler (1977)
10.1002/dvg.1020010104 / Dev. Genet / Inhibition by ammonia of intracellular cAMP accumulation in Dictyostelium discoideum: Its significance for the regulation of morphogenesis by Schindler (1979)
10.1038/356171a0 / Nature / Mutation of protein kinase A causes heterochronic development of Dictyostelium by Simon (1992)
10.1016/S0091-679X(08)61635-0 / Methods in Cell Biology / Cultivation and synchronous morphogenesis of Dictyostelium under controlled experimental conditions by Sussman (1987)
10.1016/0014-4827(78)90078-2 / Exp. Cell Res / ‘Sluggers’, a new class of morphogenetic mutants of D. discoideum by Sussman (1978)
10.1038/262717a0 / Nature / Cell differentiation without morphogenesis in Dictyostelium discoideum by Town (1976)
10.1242/dev.105.3.569 / Development / Ammonia depletion and DIF trigger stalk cell differentiation in intact Dictyostelium discoideum slugs by Wang (1989)
10.1016/0012-1606(90)90065-Q / Dev. Biol / Cytoplasmic acidification facilitates but does not mediate DIF-induced prestalk gene expression in Dictyostelium discoideum by Wang (1990)
10.1016/0012-1606(84)90294-X / Dev. Biol / Modulation of the cAMP relay in Dictyostelium by ammonia and other metabolites: possible morphogenetic consequences by Williams (1984)

Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 9:25 p.m.)
Deposited	8 months, 1 week ago (Dec. 17, 2024, 6:01 p.m.)
Indexed	8 months, 1 week ago (Dec. 17, 2024, 6:40 p.m.)
Issued	32 years, 2 months ago (June 1, 1993)
Published	32 years, 2 months ago (June 1, 1993)
Published Online	32 years, 2 months ago (June 1, 1993)
Published Print	32 years, 2 months ago (June 1, 1993)

Funders 0

None

BibTeX

@article{Inouye_1993, title={In vitro stalk cell differentiation in wild-type and ‘slugger’ mutants of Dictyostelium discoideum}, volume={118}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.118.2.523}, DOI={10.1242/dev.118.2.523}, number={2}, journal={Development}, publisher={The Company of Biologists}, author={Inouye, Kei and Gross, Julian}, year={1993}, month=jun, pages={523–526} }

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