DOI: 10.1242/dev.110.2.325. Localization of specific mRNAs in <i>Xenopus</i> embryos by whole-mount <i>in situ</i> hybridization

Localization of specific mRNAs in Xenopus embryos by whole-mount in situ hybridization

10.1242/dev.110.2.325

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

ABSTRACT We have adapted a non-radioactive technique to detect localized mRNAs in whole-mount Xenopus embryos. Synthetic antisense RNA transcribed in the presence of digoxygenin-UTP is used as a probe and is detected via an anti-digoxygenin antibody. We show that localized mRNAs can be detected from late gastrula to tadpole stages and that high as well as low abundance RNAs can be detected. The method was tested on muscle actin and α-globin RNAs, whose localization has previously been characterized. In addition, we used the method to determine the distribution of XA-1 RNA, an anterior ectoderm-specific RNA, which we show is expressed in the periphery of the cement gland as well as in the region of the hatching gland. The sequence of an XA-1 cDNA predicts a protein rich in proline and histidine.

Bibliography

Hemmati-Brivanlou, A., Frank, D., Bolce, M. E., Brown, B. D., Sive, H. L., & Harland, R. M. (1990). Localization of specific mRNAs in Xenopus embryos by whole-mount in situ hybridization. Development, 110(2), 325â330.

Authors 6

Ali Hemmati-Brivanlou (first)
Dale Frank (additional)
Margaret E. Bolce (additional)
Bob D. Brown (additional)
Hazel L. Sive (additional)
Richard M. Harland (additional)

References 23 Referenced 276

10.1242/dev.101.1.93 / Development / Posterior expression of a homeobox gene in early Xenopus embryos by Condie (1987)
10.1242/dev.105.1.61 / Development / A whole-mount immunocytochemical analysis of the expression of the intermediate filament protein vimentin in Xenopus by Dent (1989)
{'key': '2023050312024161100_DEVELOP_110_2_325C3', 'first-page': '63a', 'article-title': 'Localization of tyrosine hydroxylase to hatching gland cells of early Xenopus laevis embryos', 'volume': '109', 'author': 'Drysdale', 'year': '1989', 'journal-title': 'J. Cell Biol, (abstracts)'} / J. Cell Biol, (abstracts) / Localization of tyrosine hydroxylase to hatching gland cells of early Xenopus laevis embryos by Drysdale (1989)
{'key': '2023050312024161100_DEVELOP_110_2_325C4', 'first-page': '153', 'article-title': 'Localization of specific mRNA sequences in Xenopus laevis by in situ hybridization', 'volume': '91', 'author': 'Dworkin-Rastl', 'year': '1986', 'journal-title': 'J. Embryol. exp. Morph'} / J. Embryol. exp. Morph / Localization of specific mRNA sequences in Xenopus laevis by in situ hybridization by Dworkin-Rastl (1986)
10.1083/jcb.101.3.1094 / J. Cell Biol / Translation of mRNA injected into Xenopus oocytes is specifically inhibited by antisense RNA by Harland (1985)
10.1242/dev.106.3.611 / Development / Expression of an engrai/ed-related protein is induced in the anterior neural ectoderm of early Xenopus embryos by Hemmati-Brivanlou (1989)
10.1016/0076-6879(87)55014-5 / Meth. Enzymol / Unidirectional digestion with exonuclease III in sequence analysis by Henikoff (1987)
10.1002/j.1460-2075.1989.tb08505.x / EMBO J / MyoD expression in the forming somites is an early response to mesoderm induction in Xenopus embryos by Hopwood (1989)
10.1016/0092-8674(89)90612-0 / Cell / A Xenopus mRNA related to Drosophila twist is expressed in response to induction in the mesoderm and the neural crest by Hopwood (1989)
10.1242/dev.105.4.779 / Development / Differential gene expression in the anterior neural plate during gastrulation of Xenopus laevis by Jamrich (1989)
10.1242/dev.99.3.311 / Development / Expression of Xenopus N-CAM RNA in ectoderm is an early response to neural induction by Kintner (1987)
10.1073/pnas.80.9.2656 / Proc. natn. Acad. Sci. U.S.A / Localization of a family of mRNAs in a single cell type and its precursors in sea urchin embryos by Lynn (1983)
10.1093/nar/12.18.7035 / Nuc. Acids Res / Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter by Melton (1985)
10.1038/311716a0 / Nature / Cell type activation of actin genes in the early amphibian embryo by Mohun (1984)
{'key': '2023050312024161100_DEVELOP_110_2_325C15', 'volume-title': 'Normal table of Xenopus laevis (Daudin). North Holland, Amsterdam', 'author': 'Nieuwkoop', 'year': '1967'} / Normal table of Xenopus laevis (Daudin). North Holland, Amsterdam by Nieuwkoop (1967)
10.1242/dev.106.1.173 / Development / Bimodal and graded expression of the Xenopus homeobox gene Xhox3 during embryonic development by Ruiz (1989)
10.1016/0012-1606(90)90014-A / Devi Biol / Molecular approach to dorsoanterior development in Xenopus laevis by Sato (1990)
10.1101/gad.4.6.932 / Genes Dev. (in press) / Identification of a retinoic acid sensitive period during primary axis formation in Xenopus laevis by Sive (1990)
10.1016/0092-8674(89)90413-3 / Cell / Progressive determination during formation of anteroposterior axes in Xenopus laevis by Sive (1989)
10.1007/BF00291041 / Chromosoma / A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback by Tautz (1989)
{'key': '2023050312024161100_DEVELOP_110_2_325C21', 'first-page': '861', 'volume': '51', 'author': 'Weeks', 'year': '1987', 'journal-title': 'A maternal mRNA localized to the vegetal hemisphere of Xenopus eggs codes for a growth factor related to TGF-β Cell'} / A maternal mRNA localized to the vegetal hemisphere of Xenopus eggs codes for a growth factor related to TGF-β Cell by Weeks (1987)
10.1016/0012-1606(81)90176-7 / Devi Biol / Comparative analysis of cloned larval and adult globin cDNA sequences of Xenopus laevis by Widmer (1981)
10.1016/0092-8674(87)90664-7 / Cell / Expression of the proto-oncogene int-1 is restricted to specific neural cells in the developing mouse embryo by Wilkinson (1987)

Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 9:43 p.m.)
Deposited	2 years, 3 months ago (May 3, 2023, 8:09 a.m.)
Indexed	1 month, 2 weeks ago (July 11, 2025, 6:48 a.m.)
Issued	34 years, 10 months ago (Oct. 1, 1990)
Published	34 years, 10 months ago (Oct. 1, 1990)
Published Online	34 years, 10 months ago (Oct. 1, 1990)
Published Print	34 years, 10 months ago (Oct. 1, 1990)

Funders 0

None

BibTeX

@article{Hemmati_Brivanlou_1990, title={Localization of specific mRNAs in Xenopus embryos by whole-mount in situ hybridization}, volume={110}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.110.2.325}, DOI={10.1242/dev.110.2.325}, number={2}, journal={Development}, publisher={The Company of Biologists}, author={Hemmati-Brivanlou, Ali and Frank, Dale and Bolce, Margaret E. and Brown, Bob D. and Sive, Hazel L. and Harland, Richard M.}, year={1990}, month=oct, pages={325–330} }

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