DOI: 10.1242/dev.109.4.775. Hox-2.3 upstream sequences mediate lacZ expression in intermediate mesoderm derivatives of transgenic mice

Hox-2.3 upstream sequences mediate lacZ expression in intermediate mesoderm derivatives of transgenic mice

10.1242/dev.109.4.775

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

Abstract The mouse Hox-2.3 gene contains an Antp-like homeobox sequence and is expressed in a spatially restricted anteroposterior domain during development. To study the molecular basis of this differential gene regulation, we set out to characterize the cis-regulatory elements mediating Hox-2.3 expression during embryogenesis. We show that a fragment extending 1316 base pairs (bp) upstream of the transcription start site, thus corresponding to the Hox-2.4/Hox-2.3 intergenic sequences is capable of mediating luciferase gene transcription in transfected cells in vitro and lacZ expression in transgenic mice. The β-galactosidase-staining pattern in embryos was found to be strikingly similar to the Hox-2.3 in situ hybridization pattern in intermediate mesoderm derivatives: high levels of both Hox-2.3 transcripts and β-galactosidase activity were found in the mesonephric duct-derived epithelium of the meso- and metanephric kidney and associated ducts, from the time these structures first appeared on throughout development. The transgene apparently lacks sequences needed for correct Hox-2.3 expression in somitic and lateral plate mesoderm and in neurectoderm. These results document the involvement of distinct regulatory elements in Hox gene expression in subsets of cells with distinct developmental fate, situated at similar positions along the anteroposterior axis of the embryo.

Bibliography

Kress, C., Vogels, R., Graaff, W. D., Bonnerot, C., Meijlink, F., Nicolas, J.-F., & Deschamps, J. (1990). Hox-2.3 upstream sequences mediate lacZ expression in intermediate mesoderm derivatives of transgenic mice. Development, 109(4), 775â786.

Authors 7

Chantal Kress (first)
Ronald Vogels (additional)
Wim De Graaff (additional)
Claire Bonnerot (additional)
Frits Meijlink (additional)
Jean-François Nicolas (additional)
Jacqueline Deschamps (additional)

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Dates

Type	When
Created	4 years, 4 months ago (April 24, 2021, 5:17 a.m.)
Deposited	2 years, 3 months ago (May 3, 2023, 7:02 a.m.)
Indexed	1 week ago (Aug. 23, 2025, 1:22 a.m.)
Issued	35 years ago (Aug. 1, 1990)
Published	35 years ago (Aug. 1, 1990)
Published Online	35 years ago (Aug. 1, 1990)
Published Print	35 years ago (Aug. 1, 1990)

Funders 0

None

BibTeX

@article{Kress_1990, title={Hox-2.3 upstream sequences mediate lacZ expression in intermediate mesoderm derivatives of transgenic mice}, volume={109}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.109.4.775}, DOI={10.1242/dev.109.4.775}, number={4}, journal={Development}, publisher={The Company of Biologists}, author={Kress, Chantal and Vogels, Ronald and Graaff, Wim De and Bonnerot, Claire and Meijlink, Frits and Nicolas, Jean-François and Deschamps, Jacqueline}, year={1990}, month=aug, pages={775–786} }

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