DOI: 10.1242/dev.108.3.435. Position-specific activity of the Hox1.1 promoter in transgenic mice

Position-specific activity of the Hox1.1 promoter in transgenic mice

10.1242/dev.108.3.435

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

Abstract During development, positional values have to be assigned to groups of cells. The murine Hox genes are a class of genes that are predicted to be involved at some stage in this process. During embryogenesis they are expressed in distinct overlapping region- and stagespecific patterns and therefore must be regulated in response to positional information. In this study, we have analysed the activity of Hox1.1 promoter sequences in transgenic mice. The use of lacZ as a marker allows a detailed analysis of expression at the single cell level during early embryonic development. We show that 3.6 kbp of promoter and 1.7 kbp of 3’ sequences provide sufficient regulatory information to express a transgene in a spatial and temporal manner indistinguishable from the endogenous Hox1.1 gene during the period of development when Hox1.1 expression Is established. The activation occurs in a strict order in specific ectodermal and mesodermal domains. Within each of these domains the transgene is activated over a period of four hours apparently randomly in single cells. In a following second period, Hox1.1 and transgene expression patterns diverge. In this period, transgene expression persists in many mesodermally derived cells that do not express Hox1.1 indicating the absence of a negative regulatory element in the transgene. The anterior boundary of transgene expression is identical to that of Hox1.1. However, no posterior boundary of transgene expression is set, suggesting that a separate element absent from the transgene specifies this boundary.

Bibliography

PÃ¼schel, A. W., Balling, R., & Gruss, P. (1990). Position-specific activity of the Hox1.1 promoter in transgenic mice. Development, 108(3), 435â442.

Authors 3

Andreas W. Püschel (first)
Rudi Balling (additional)
Peter Gruss (additional)

References 25 Referenced 60

10.1242/dev.101.1.1 / Development / The molecular basis for metameric pattern in the Drosophila embryo by Akam (1987)
10.1016/0092-8674(89)90848-9 / Cell / Craniofacial abnormalities induced by ectopic expression of the homeobox gene Hox 1.1 in transgenic mice by Balling (1989)
10.1093/oxfordjournals.humrep.a136802 / Human Reproduction / Organization of human homeobox genes by Boncinelu (1988)
10.1016/0092-8674(85)90010-8 / Cell / Clustered horneo boxes are differentially expressed during murine development by Colberg-Poley (1985)
10.1016/0092-8674(88)90131-6 / Cell / Posterior pattern formation in C. elegans involves position-specific expression of a gene containing a homeobox by Costa (1988)
10.1098/rstb.1930.0004 / Phil. Trans. R. Soc. Land. B / The development of the vertebral column in mammals, as illustrated by its development in Mus musculus by Dawes (1930)
10.1016/0092-8674(89)90954-9 / Cell / Determination of axial polarity in the vertebrate embryo: homeodomain proteins and homeogenetic induction by De Robertis (1989)
10.1002/j.1460-2075.1987.tb02602.x / EMBO J / Specific expression of the Hox 1.3 horneo box gene in murine embryonic structures originating from or induced by the mesoderm by Dony (1987)
10.1016/S0168-9525(88)80003-9 / Trends in Genetics / Do multigene families regulate vertebrate development? by Dressler (1988)
10.1016/0168-9525(89)90048-6 / Trends in Genetics / An update on the vertebrate homeobox by Dressler (1988)
10.1111/j.1432-0436.1989.tb00747.x / Differentiation, in press / Anterior boundaries of Hox gene expression in mesoderm derived structures correlate with the linear gene order along the chromosome by Dressler (1989)
10.1146/annurev.ge.20.120186.001051 / Ann. Rev. Genet. / Homeotic genes and the homeobox by Gehring (1986)
10.1101/gad.2.12a.1513 / Genes & Development / The Pou domain: a large conserved region in the mammalian pit-1, oct-1, oct-2, and Caenorhabditis elegans unc-86 gene products by Herr (1988)
{'key': '2024022601431290700_DEVELOP_108_3_435C14', 'article-title': 'Manipulating the mouse embryo', 'volume-title': 'Cold Spring Harbor Laboratory.', 'author': 'Hogan', 'year': '1986'} / Cold Spring Harbor Laboratory. / Manipulating the mouse embryo by Hogan (1986)
10.1101/gad.2.7.773 / Genes & Development / Expression of horneo box genes during mouse development: a review by Holland (1988)
10.1038/335025a0 / Nature, Lond. / The molecular genetics of embryonic pattern formation in Drosophila by Ingham (1988)
10.1073/pnas.84.15.5306 / Proc. natn. Acad. Sci. U.S.A. / Primary structure and nuclear localization of a murine homeodomain protein by Kessel (1987)
10.1016/0092-8674(88)90209-7 / Cell / Homeobox proteins as sequencespecific transcription factors by Levine (1988)
10.1242/dev.104.Supplement.187 / Development / Expression of homeobox gene Hox1.1 during mouse embryogenesis by Mahon (1988)
10.1038/325021b0 / Nature, Lond. / Nomenclature for homeobox-containing genes by Martin (1987)
10.1016/0092-8674(89)90969-0 / Cell / Involvement of the Xenopus homeobox gene Xhox3 in pattern formation along the anterior-posterior axis by Ruiz (1989)
10.1016/0012-1606(87)90236-3 / Devi Biol. / Formation of the dorsal root ganglia in the avian embryo: segmental origin and migratory behavior of neural crest progenitor cells by Teillet (1987)
10.1016/0092-8674(88)90174-2 / Cell / mec-3, a homeobox-containing gene that specifies differentiation of the touch receptor neurons in C. elegans by Way (1988)
10.1016/0968-0004(89)90043-1 / Trends in Biochem. Sci. / Vertebrate homeodomain proteins: families of region-specific transcription factors by Wright (1989)
10.1016/0896-6273(88)90167-5 / Neuron / Spatial regulation of homeobox gene fusions in the embryonic central nervous system of transgenic mice by Zakany (1988)

Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 8:40 p.m.)
Deposited	1 year, 6 months ago (Feb. 25, 2024, 8:43 p.m.)
Indexed	1 year, 2 months ago (June 4, 2024, 3:13 p.m.)
Issued	35 years, 5 months ago (March 1, 1990)
Published	35 years, 5 months ago (March 1, 1990)
Published Online	35 years, 5 months ago (March 1, 1990)
Published Print	35 years, 5 months ago (March 1, 1990)

Funders 0

None

BibTeX

@article{P_schel_1990, title={Position-specific activity of the Hox1.1 promoter in transgenic mice}, volume={108}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.108.3.435}, DOI={10.1242/dev.108.3.435}, number={3}, journal={Development}, publisher={The Company of Biologists}, author={Püschel, Andreas W. and Balling, Rudi and Gruss, Peter}, year={1990}, month=mar, pages={435–442} }

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