DOI: 10.1242/dev.108.2.229. Mesoderm induction and the control of gastrulation in <i>Xenopus laevis:</i> the roles of fibronectin and integrins

Mesoderm induction and the control of gastrulation in Xenopus laevis: the roles of fibronectin and integrins

10.1242/dev.108.2.229

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

Abstract Exposure of isolated Xenopus animal pole ectoderm to the XTC mesoderm-inducing factor (XTC-MIF) causes the tissue to undergo gastrulation-like movements. In this paper, we take advantage of this observation to investigate the control of various aspects of gastrulation in Xenopus. Blastomeres derived from induced animal pole regions are able, like marginal zone cells, but unlike control animal pole blastomeres, to spread and migrate on a fibronectin-coated surface. Dispersed animal pole cells are also able to respond to XTC-MIF in this way; this is one of the few mesoderm-specific responses to induction that has been observed in single cells. The ability of induced animal pole cells to spread on fibronectin is abolished by the peptide GRGDSP. However, the elongation of intact explants is unaffected by this peptide. This may indicate that fibronectin-me-diated cell migration is not required for convergent extension. We have investigated the molecular basis of XTC-MIF-induced gastrulation-like movements by measuring rates of synthesis of fibronectin and of the integrin fit chain in induced and control explants. No significant differences were observed, and this suggests that gastrulation is not initiated simply by control of synthesis of these molecules. In future work, we intend to investigate synthesis of other integrin subunits and to examine possible post-translational modifications to fibronectin and the integrins.

Bibliography

Smith, J. C., Symes, K., Hynes, R. O., & DeSimone, D. (1990). Mesoderm induction and the control of gastrulation in Xenopus laevis: the roles of fibronectin and integrins. Development, 108(2), 229â238.

Authors 4

J. C. Smith (first)
K. Symes (additional)
R. O. Hynes (additional)
D. DeSimone (additional)

References 58 Referenced 89

10.1016/0014-5793(88)80580-5 / FEBS Let. / Transforming growth factor beta regulates the expression of different fibronectin isoforms in normal human cultured fibroblasts by Balza (1988)
10.1111/j.1432-1033.1974.tb03599.x / Eur. J. Biochem. / A film detection method for 3H-labelled proteins and nucleic acids in polyacrylamide gels by Bonner (1974)
10.1007/BF00217407 / Cell Tissue Res. / Fibronectin in early amphibian embryos. Migrating mesodermal cells contact fibronectin established prior to gastrulation by Boucaut (1983)
10.1038/307364a0 / Nature, Lond. / Prevention of gastrulation but not neurulation by antibodies to fibronectin in amphibian embryos by Boucaut (1984)
10.1083/jcb.99.5.1822 / J. Cell Biol. / Biologically active synthetic peptides as probes of embryonic development: A competitive peptide inhibitor of fibronectin function inhibits gastrulation in amphibian embryos and neural crest migration in avian embryos by Boucaut (1984)
10.1016/S0012-1606(89)80012-0 / Devl Biol. / Gastrulation and larval pattern in Xenopus after blastocoelic injection of a Xenopus inducing factor: experiments testing models for the normal organization of mesoderm by Cooke (1989)
10.1242/dev.101.4.893 / Development / The organization of mesodermal pattern in Xenopus laevis: experiments using a Xenopus mesoderm-inducing factor by Cooke (1987)
10.1007/BF00218997 / Cell Tissue Res. / Synthesis and distribution of laminin-related polypeptides in early amphibian embryos by Darribère (1986)
10.1016/0012-1606(88)90252-7 / Devl Biol. / The 140-kDa fibronectin receptor complex is required for mesodermal cell adhesion during gastrulation in the amphibian Pleurodeles waltii by Darribère (1988)
{'key': '2023050311341908000_DEVELOP_108_2_229C10', 'article-title': 'The role of growth factors in embryonic induction in amphibians', 'volume-title': 'Curr. Topics devl Biol. In Press', 'author': 'Dawid', 'year': '1989'} / Curr. Topics devl Biol. In Press / The role of growth factors in embryonic induction in amphibians by Dawid (1989)
10.1016/S0021-9258(18)60720-3 / J. biol. Chem. / Xenopus laevis integrins. Structural conservation and evolutionary divergence of integrin ft subunits by Desimone (1988)
10.1002/j.1460-2075.1988.tb03119.x / EMBO J. / Attachment, spreading and locomotion of avian neural crest cells are mediated by multiple adhesion sites on fibronectin molecules by Dufour (1988)
10.1083/jcb.109.2.903 / J. Cell Biol. / Reappearance of an embryonic pattern of fibronectin splicing during wound healing in the adult rat by Ffrench-Constant (1989)
10.1016/0012-1606(89)90122-X / Devl Biol. / Clonal analysis of mesoderm induction in Xenopus laevis by Godsave (1989)
10.1242/dev.99.3.285 / Development / Embryonic induction - molecular prospects by Gurdon (1987)
10.1038/336772a0 / Nature / A community effect in animal development by Gurdon (1988)
10.1016/S0021-9258(18)94206-7 / J. biol. Chem. / Effects of modifications of the RGD sequence and its context on recognition by the fibronectin receptor by Hautanen (1989)
10.1016/0092-8674(81)90385-8 / Cell / Primordial germ cells of Xenopus embryos: the role of fibronectin in their adhesion during migration by Heasman (1981)
10.1016/S0021-9258(17)31269-3 / J. biol. Chem. / Regulation of cell-adhesion receptors by transforming growth factor beta - concominant regulation of integrins that share a common beta-1 subunit by Heino (1989)
10.1016/0092-8674(87)90233-9 / Cell / Integrins: A family of cell surface receptors by Hynes (1987)
10.1016/S0021-9258(17)35666-1 / J. biol. Chem. / Transforming growth factor-/? stimulates the expression of fibronectin and collagen and their incorporation into the extracellular matrix by Ignotz (1986)
10.1016/0092-8674(87)90146-2 / Cell / Cell adhesion protein receptors as targets for transforming growth factor-/? action by Ignotz (1987)
10.1093/icb/24.3.589 / Amer. Zool. / The cellular basis of gastrulation in Xenopus laevis: Active, postinvolution convergence and extension by mediolateral interdigitation by Keller (1984)
10.1007/978-1-4613-2141-5_7 / The Cellular Basis of Morphogenesis, / The Cellular Basis of Amphibian Gastrulation. In Developmental Biology: A Comprehensive Synthesis, by Keller (1986)
10.1242/dev.103.1.193 / Development / Regional expression, pattern and timing of convergence and extension during gastrulation of Xenopus laevis by Keller (1988)
10.1242/dev.89.supplement.185 / J. Embryol. exp. Morph. 89 Supplement 185-209 / The function and mechanism of convergent extension during gastrulation of Xenopus laevis by Keller (1985)
10.1242/jcs.1987.Supplement_8.21 / J. Cell Sci. Suppl / Cell behaviour during active cell rearrangement: evidence and speculations by Keller (1987)
10.1016/S0012-1606(89)80024-7 / Devl Biol. / Mediolateral cell intercalation in the dorsal axial mesoderm of Xenopus laevis by Keller (1989)
10.1016/0045-6039(88)90083-8 / Cell Diff. / Factors related to the initiation of cell migration along the inner surface of the blastocoelic wall during amphibian gastrulation by Komazaki (1988)
10.1002/j.1460-2075.1985.tb04105.x / EMBO J. / Developmental regulation of a gastrula-specific gene injected into fertilized Xenopus eggs by Krieg (1985)
10.1038/227680a0 / Nature, Lond. / Cleavage of structural proteins during the assembly of the head of bacteriophage T4 by Laemmli (1970)
10.1016/0092-8674(84)90353-2 / Cell / Temporal and spatial regulation of fibronectin in early Xenopus development by Lee (1984)
10.1083/jcb.106.5.1765 / J. Cell Biol. / Antibodies to the conserved cytoplasmic domain of the integrin β1 subunit react with proteins in vertebrates, invertebrates, and fungi by Marcantonio (1988)
10.1126/science.2442813 / Science / Accelerated healing of incisional wounds in rats induced by transforming growth factor- fi by Mustoe (1987)
10.1242/jcs.86.1.109 / J. Cell Sci. / Presumptive mesoderm cells from Xenopus laevis gastrulae attach to and migrate on substrata coated with fibronectin or laminin by Nakatsuji (1986)
{'key': '2023050311341908000_DEVELOP_108_2_229C36', 'article-title': 'Laminin fibrils in newt gastrulae visualized by the immunofluorescent staining', 'volume-title': 'Dev. Growth Diff. 27, 639-643', 'author': 'Nakatsuji', 'year': '1985'} / Dev. Growth Diff. 27, 639-643 / Laminin fibrils in newt gastrulae visualized by the immunofluorescent staining by Nakatsuji (1985)
10.1016/0012-1606(85)90395-1 / Devi Biol. / Fibronectin visualized by scanning electron microscopy on the substratum for cell migration in Xenopus laevis gastrulae by Nakatsuji (1985)
10.1016/0092-8674(82)90272-0 / Cell / A major developmental transition in early Xenopus embryos: I. Characterization and timing of cellular changes at the midblastula stage by Newport (1982)
{'key': '2023050311341908000_DEVELOP_108_2_229C39', 'volume-title': 'Normal Table of Xenopus laevis (Daudin), 2nd', 'author': 'Nieuwkoop', 'year': '1967'} / Normal Table of Xenopus laevis (Daudin), 2nd by Nieuwkoop (1967)
{'key': '2023050311341908000_DEVELOP_108_2_229C40', 'first-page': '4297', 'article-title': 'Alternative splicing of chicken fibronectin in embryos and in normal and transformed cells', 'volume': '7', 'author': 'Norton', 'year': '1987', 'journal-title': 'Mol. cell. Biol.'} / Mol. cell. Biol. / Alternative splicing of chicken fibronectin in embryos and in normal and transformed cells by Norton (1987)
10.1038/309030a0 / Nature, Lond. / Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule by Pierschbacher (1984)
10.1016/0092-8674(89)90902-1 / Cell / Changes in integrin receptors on oncogenically transformed cells by Plantefaber (1989)
10.1016/S0021-9258(18)68822-2 / J. biol. Chem. / Transforming growth factor fi stimulates the expression of fibronectin and of both subunits of the human fibronectin receptor by cultured human lung fibroblasts by Roberts (1988)
10.1016/0092-8674(89)90335-8 / Cell / Mix. 1, a homeobox mRNA inducible by mesoderm inducers, is expressed mostly in the presumptive endodermal cells of Xenopus embryos by Rosa (1989)
10.1126/science.3422517 / Science / Mesoderm induction in amphibians: the role of TGFjÉ-like factors by Rosa (1988)
10.1126/science.6688681 / Science / Differential gene expression in the gastrula of Xenopus laevis by Sargent (1983)
10.1016/0012-1606(86)90399-4 / Devi Biol. / Cell interactions and the control of gene activity during early development of Xenopus laevis by Sargent (1986)
{'key': '2023050311341908000_DEVELOP_108_2_229C48', 'first-page': '1', 'article-title': 'The mechanism of amphibian gastrulation: I. Gastrulation-promoting interactions between various regions of an anuran egg (Hyla regilla)', 'volume': '51', 'author': 'Schechtman', 'year': '1942', 'journal-title': 'Univ. Calif. Publ. Zool.'} / Univ. Calif. Publ. Zool. / The mechanism of amphibian gastrulation: I. Gastrulation-promoting interactions between various regions of an anuran egg (Hyla regilla) by Schechtman (1942)
10.1242/dev.105.2.351 / Development / Initiation of mesodermal cell migration and spreading relative to gastrulation in the urodele amphibian Pleurodeles waltl by Shi (1989)
{'key': '2023050311341908000_DEVELOP_108_2_229C50', 'first-page': '289', 'article-title': 'Regional biosynthetic markers in the early amphibian embryo', 'volume': '80', 'author': 'Slack', 'year': '1984', 'journal-title': 'J. Embryol. èxp. Morph.'} / J. Embryol. èxp. Morph. / Regional biosynthetic markers in the early amphibian embryo by Slack (1984)
10.1242/dev.99.1.3 / Development / A mesoderm-inducing factor is produced by a Xenopus cell line by Smith (1987)
10.1242/dev.105.4.665 / Development / Mesoderm induction and mesoderm-inducing factors in early amphibian development by Smith (1989)
{'key': '2023050311341908000_DEVELOP_108_2_229C53', 'first-page': '299', 'article-title': 'Dorsalization and neural induction: properties of the organizer in Xenopus laevis', 'volume': '78', 'author': 'Smith', 'year': '1983', 'journal-title': 'J. Embryol. exp. Morph.'} / J. Embryol. exp. Morph. / Dorsalization and neural induction: properties of the organizer in Xenopus laevis by Smith (1983)
10.1242/dev.103.3.591 / Development / Purification, partial characterization and biological properties of the XTC mesoderm-inducing factor by Smith (1988)
10.1242/dev.101.2.339 / Development / Gastrulation movements provide an early marker of mesoderm induction in Xenopus laevis by Symes (1987)
10.1242/dev.104.4.609 / Development / Mesoderm induction in Xenopus laevis: responding cells must be in contact for mesoderm formation but suppression of epidermal differentiation can occur in single cells by Symes (1988)
10.1016/0012-1606(88)90424-1 / Devi Biol. / Differential interaction of Xenopus embryonic cells with fibronectin in vitro. by W1Nklbauer (1988)
10.1083/jcb.99.1.29 / J. Cell Biol. / Dualistic nature of adhesive protein function: fibronectin and its biologically active peptide fragments can autoinhibit fibronectin function by Yamada (1984)

Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 8:40 p.m.)
Deposited	2 years, 4 months ago (May 3, 2023, 7:41 a.m.)
Indexed	1 month, 4 weeks ago (July 8, 2025, 7:33 a.m.)
Issued	35 years, 7 months ago (Feb. 1, 1990)
Published	35 years, 7 months ago (Feb. 1, 1990)
Published Online	35 years, 7 months ago (Feb. 1, 1990)
Published Print	35 years, 7 months ago (Feb. 1, 1990)

Funders 0

None

BibTeX

@article{Smith_1990, title={Mesoderm induction and the control of gastrulation in Xenopus laevis: the roles of fibronectin and integrins}, volume={108}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.108.2.229}, DOI={10.1242/dev.108.2.229}, number={2}, journal={Development}, publisher={The Company of Biologists}, author={Smith, J. C. and Symes, K. and Hynes, R. O. and DeSimone, D.}, year={1990}, month=feb, pages={229–238} }

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      "author": "Bonner",
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      "journal-title": "Eur. J. Biochem."
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      "author": "Boucaut",
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      "author": "Heino",
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      "author": "Hynes",
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      "author": "Ignotz",
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      "author": "Keller",
      "year": "1984",
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      "author": "Krieg",
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