DOI: 10.1242/dev.105.4.733. An assessment of the developmental potential of embryonic stem cells in the midgestation mouse embryo

An assessment of the developmental potential of embryonic stem cells in the midgestation mouse embryo

10.1242/dev.105.4.733

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

ABSTRACT Embryonic stem cells (ES) cells were injected into host blastocysts either in groups of 10–15 cells or as single cells in order to test their developmental potential in the developing embryo. The analysis of midgestation chimaeras, by electrophoretic separation of glucose phosphate isomerase (GPI) isozymes, showed that ES cells were capable of colonizing trophectoderm and primitive endoderm derivatives at a low frequency, as well as producing a high rate of chimaerism in tissues of the fetus and extraembryonlc mesoderm.

Bibliography

Beddington, R. S. P., & Robertson, E. J. (1989). An assessment of the developmental potential of embryonic stem cells in the midgestation mouse embryo. Development, 105(4), 733â737.

Authors 2

R. S. P. Beddington (first)
E. J. Robertson (additional)

References 27 Referenced 466

{'key': '2023050311221656700_DEVELOP_105_4_733C1', 'first-page': '113', 'article-title': 'Production and analysis of chimaeras', 'volume-title': 'In Teratocarcinomas and Embryonic Stem Cells: A Practical Approach', 'author': 'Bradley', 'year': '1987'} / In Teratocarcinomas and Embryonic Stem Cells: A Practical Approach / Production and analysis of chimaeras by Bradley (1987)
10.1038/309255a0 / Nature, Lond / Formation of germ line chimaeras from embryo-derived teratocarcinoma cell lines by Bradley (1984)
{'key': '2023050311221656700_DEVELOP_105_4_733C3', 'first-page': '27', 'article-title': 'tn vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium', 'volume': '87', 'author': 'Doetschman', 'year': '1985', 'journal-title': 'J. Embryol. exp. Morph'} / J. Embryol. exp. Morph / tn vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium by Doetschman (1985)
10.1073/pnas.75.2.946 / Proc. natl. Acad. Sci., U.S.A / Assignement of genes to regions of mouse chromosomes by Eicher (1978)
10.1002/dvg.1020040303 / Dev. Genet / Production and properties of mouse trisomy 15 ↔ diploid chimaeras by Epstein (1984)
10.1073/pnas.79.14.4376 / Proc. natn. Acad. Sci., U.S.A / Production of viable adult trisomy 17"-"diploid mouse chimaeras by Epstein (1982)
{'key': '2023050311221656700_DEVELOP_105_4_733C7', 'first-page': '93', 'article-title': 'EK cell contribution to chimeric mice: from tissue culture to sperm', 'volume-title': 'Banbury Report 20. Genetic Manipulation of the Early Mammalian Embryo', 'author': 'Evans', 'year': '1985'} / Banbury Report 20. Genetic Manipulation of the Early Mammalian Embryo / EK cell contribution to chimeric mice: from tissue culture to sperm by Evans (1985)
10.1038/292154a0 / Nature. Lond / Establishment in culture of pluripotential cells from mouse embryos by Evans (1981)
{'key': '2023050311221656700_DEVELOP_105_4_733C9', 'first-page': '186', 'article-title': 'Pluripotential cells grown directly from normal mouse embryos', 'volume': '2', 'author': 'Evans', 'year': '1983', 'journal-title': 'Cancer Surveys'} / Cancer Surveys / Pluripotential cells grown directly from normal mouse embryos by Evans (1983)
{'key': '2023050311221656700_DEVELOP_105_4_733C10', 'first-page': '115', 'article-title': 'Cell lineage of the mouse embryo and embryonal carcinoma cells: Forssman antigen and patterns of protein synthesis', 'volume-title': 'In Cell Lineages, Stem Cells, and Cell Determination. INSERM Symposium No', 'author': 'Evans', 'year': '1979'} / In Cell Lineages, Stem Cells, and Cell Determination. INSERM Symposium No / Cell lineage of the mouse embryo and embryonal carcinoma cells: Forssman antigen and patterns of protein synthesis by Evans (1979)
{'key': '2023050311221656700_DEVELOP_105_4_733C11', 'first-page': '175', 'article-title': 'Investigation of cell lineage and differentiation in the extraembryonic endoderm of the mouse embryo', 'volume': '68', 'author': 'Gardner', 'year': '1982', 'journal-title': 'J. Embryol. exp. Morph'} / J. Embryol. exp. Morph / Investigation of cell lineage and differentiation in the extraembryonic endoderm of the mouse embryo by Gardner (1982)
{'key': '2023050311221656700_DEVELOP_105_4_733C12', 'first-page': '163', 'article-title': 'Clonal analysis of early mammalian development', 'volume': '312', 'author': 'Gardner', 'year': '1985', 'journal-title': 'Phil. Trans. R. Soc., B'} / Phil. Trans. R. Soc., B / Clonal analysis of early mammalian development by Gardner (1985)
{'key': '2023050311221656700_DEVELOP_105_4_733C13', 'first-page': '303', 'article-title': 'Regeneration of endoderm from primitive ectoderm: fact or artifact?', 'volume': '88', 'author': 'Gardner', 'year': '1985', 'journal-title': 'J. Embryol. exp. Morph'} / J. Embryol. exp. Morph / Regeneration of endoderm from primitive ectoderm: fact or artifact? by Gardner (1985)
{'key': '2023050311221656700_DEVELOP_105_4_733C14', 'article-title': 'Multi-lineage “stem” cells in the mammalian embryo', 'volume-title': 'J. Cell Sci. Suppl. (in press)', 'author': 'Gardner', 'year': '1989'} / J. Cell Sci. Suppl. (in press) / Multi-lineage “stem” cells in the mammalian embryo by Gardner (1989)
10.1016/0143-4004(88)90048-3 / Placenta / Effect of delayed implantation on differentiation of the extra-embryonic endoderm in the mouse blastocyst by Gardner (1988)
{'key': '2023050311221656700_DEVELOP_105_4_733C16', 'first-page': '349', 'article-title': 'Clonal analysis of X-chromosome inactivation and the origin of the germ line in the mouse embryo', 'volume': '88', 'author': 'Gardner', 'year': '1985', 'journal-title': 'J. Embryol. exp. Morph'} / J. Embryol. exp. Morph / Clonal analysis of X-chromosome inactivation and the origin of the germ line in the mouse embryo by Gardner (1985)
{'key': '2023050311221656700_DEVELOP_105_4_733C17', 'first-page': '561', 'article-title': 'Origin of the ectopiacental cone and secondary giant cells in mouse blastocysts reconstituted from isolated trophoblast and inner cell mass', 'volume': '30', 'author': 'Gardner', 'year': '1973', 'journal-title': 'J. Embryol. exp. Morph'} / J. Embryol. exp. Morph / Origin of the ectopiacental cone and secondary giant cells in mouse blastocysts reconstituted from isolated trophoblast and inner cell mass by Gardner (1973)
{'key': '2023050311221656700_DEVELOP_105_4_733C18', 'first-page': '141', 'article-title': 'Investigation of the fate of 4-5d inner cell mass cells by blastocyst injection', 'volume': '52', 'author': 'Gardner', 'year': '1979', 'journal-title': 'J. Embryol. exp. Morph'} / J. Embryol. exp. Morph / Investigation of the fate of 4-5d inner cell mass cells by blastocyst injection by Gardner (1979)
{'key': '2023050311221656700_DEVELOP_105_4_733C19', 'first-page': '115', 'article-title': 'F9 teratocarcinoma cells as a model for the differentiation of parietal and visceral endoderm in the mouse embryo', 'volume': '2', 'author': 'Hogan', 'year': '1983', 'journal-title': 'Cancer Surveys'} / Cancer Surveys / F9 teratocarcinoma cells as a model for the differentiation of parietal and visceral endoderm in the mouse embryo by Hogan (1983)
10.1073/pnas.78.12.7634 / Proc. natn. Acad. Sci., U.S.A / Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells by Martin (1981)
10.1017/S0016672300020309 / Genet. Res. Camb / GPI expression in female germ cells of the mouse by McLaren (1981)
{'key': '2023050311221656700_DEVELOP_105_4_733C22', 'first-page': '199', 'article-title': 'Lineage analysis of inner cell mass and trophectoderm using microsurgically reconstituted mouse blastocysts', 'volume': '68', 'author': 'Papaioannou', 'year': '1982', 'journal-title': 'J. Embryol. exp. Morph'} / J. Embryol. exp. Morph / Lineage analysis of inner cell mass and trophectoderm using microsurgically reconstituted mouse blastocysts by Papaioannou (1982)
{'key': '2023050311221656700_DEVELOP_105_4_733C23', 'first-page': '165', 'article-title': 'Effects of the embryonic environment on proliferation and differentiation of embryonal carcinoma cells', 'volume': '2', 'author': 'Papaioannou', 'year': '1983', 'journal-title': 'Cancer Surveys'} / Cancer Surveys / Effects of the embryonic environment on proliferation and differentiation of embryonal carcinoma cells by Papaioannou (1983)
{'key': '2023050311221656700_DEVELOP_105_4_733C24', 'first-page': '71', 'article-title': 'Embryo-derived stem cell lines', 'volume-title': 'In Teratocarcinomas and Embryonic Stem Cells: A Practical Approach', 'author': 'Robertson', 'year': '1987'} / In Teratocarcinomas and Embryonic Stem Cells: A Practical Approach / Embryo-derived stem cell lines by Robertson (1987)
{'key': '2023050311221656700_DEVELOP_105_4_733C25', 'first-page': '475', 'article-title': 'Production of permanent cell lines from early embryos and their use in studying developmental problems', 'volume-title': 'In Experimental Approaches to Mammalian Embryonic Development', 'author': 'Robertson', 'year': '1986'} / In Experimental Approaches to Mammalian Embryonic Development / Production of permanent cell lines from early embryos and their use in studying developmental problems by Robertson (1986)
10.1038/269515a0 / Nature, Lond / Viable chimaeras produced from normal and parthenogenetic mouse embryos by Stevens (1977)
10.1071/BI9691065 / Aust. J. biol / Storage of two cell mouse embryos in vitro by Whittingham (1969)

Dates

Type	When
Created	4 years, 4 months ago (April 25, 2021, 8:39 p.m.)
Deposited	2 years, 3 months ago (May 3, 2023, 7:23 a.m.)
Indexed	21 hours, 51 minutes ago (Aug. 26, 2025, 2:46 a.m.)
Issued	36 years, 4 months ago (April 1, 1989)
Published	36 years, 4 months ago (April 1, 1989)
Published Online	36 years, 4 months ago (April 1, 1989)
Published Print	36 years, 4 months ago (April 1, 1989)

Funders 0

None

BibTeX

@article{Beddington_1989, title={An assessment of the developmental potential of embryonic stem cells in the midgestation mouse embryo}, volume={105}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.105.4.733}, DOI={10.1242/dev.105.4.733}, number={4}, journal={Development}, publisher={The Company of Biologists}, author={Beddington, R. S. P. and Robertson, E. J.}, year={1989}, month=apr, pages={733–737} }

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  "journal-issue": {
    "issue": "4",
    "published-print": {
      "date-parts": [
        [
          1989,
          4,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1242/dev.105.4.733",
  "relation": {},
  "ISSN": [
    "0950-1991",
    "1477-9129"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1989,
        4,
        1
      ]
    ]
  }
}