DOI: 10.1242/dev.105.1.147. Presence of basic fibroblast growth factor in the early <i>Xenopus</i> embryo

Presence of basic fibroblast growth factor in the early Xenopus embryo

10.1242/dev.105.1.147

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

Abstract Mesoderm-inducing activity can be extracted from Xenopus embryos, eggs or whole ovary. It binds to heparin and can be neutralized by heparin or anti-bFGF but not by anti-TGFβ. Two molecular forms can be identified by Western blotting and have molecular weights of about 19 and 14K. The content in embryos is about 7 units g-1 (approximately 7 ng ml-1) which would be sufficient for it to be acting as an endogenous inducer of ventral mesoderm. Attempts to detect TGFβ-like inducing factors in embryos were not successful.

Bibliography

Slack, J. M. W., & Isaacs, H. V. (1989). Presence of basic fibroblast growth factor in the early Xenopus embryo. Development, 105(1), 147â153.

Authors 2

J. M. W. Slack (first)
H. V. Isaacs (additional)

References 22 Referenced 134

10.1126/science.2425435 / Science / Nucleotide sequence of a bovine clone encoding the angiogenic protein, basic fibroblast growth factor by Abraham (1986)
10.1016/S0021-9258(18)32345-7 / J. biol Chem. / Transforming growth factor beta in human platelets by Assoian (1983)
10.1242/dev.100.2.279 / Development / Regional specification within the mesoderm of early embryos of Xenopus laevis by Dale (1987)
{'key': '2023050311113154300_DEVELOP_105_1_147C4', 'first-page': '289', 'article-title': 'Mesoderm induction in Xenopus laevis. A quantitative study using a cell lineage label and tissue specific antibodies', 'volume': '89', 'author': 'Dale', 'year': '1985', 'journal-title': 'J. Embryol. exp. Morph.'} / J. Embryol. exp. Morph. / Mesoderm induction in Xenopus laevis. A quantitative study using a cell lineage label and tissue specific antibodies by Dale (1985)
10.1016/0092-8674(88)90183-3 / Cell / The bicoid protein determines position in the Drosophila embryo in a concentrationdependent manner by Driever (1988)
10.1073/pnas.82.19.6507 / Proc. natn. Acad. Sci. U.S.A. / Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino terminal sequence of bovine brain acidic FGF by Esch (1985)
10.1242/dev.102.3.555 / Development / Mesoderm inducing factors: a small class of molecules by Isaacs (1988)
10.1073/pnas.81.22.6963 / Proc. natn. Acad. Sci. U.S.A. / Isolation of brain fibroblast growth factor by heparin Sepharose affinity chromatography: identity with pituitary fibroblast growth by Gospodarowicz (1984)
10.1016/S0092-8674(85)80072-6 / Cell / The activation of muscle specific actin genes in Xenopus development by an induction between animal and vegetal cells of a blastula by Gurdon (1985)
10.1002/elps.1150060302 / Electrophoresis / Simplified methods for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining by Heukeshoven (1985)
10.1242/dev.101.3.557 / Development / The development of animal cap cells in Xenopus: a measure of the start of animal cap competence to form mesoderm by Jones (1987)
10.1016/0012-1606(76)90127-5 / Devi Biol. / Vital dye mapping of the gastrula and neurula of Xenopus laevis. II. Prospective areas and morphogenetic movements of the deep layer by Keller (1976)
10.1016/0092-8674(87)90110-3 / Cell / Synergistic induction of mesoderm by FGF and TGF-/J and the identification of an mRNA coding for FGF in the early Xenopus embryo by Kimelman (1987)
10.1016/0003-2697(86)90487-2 / Anal. Biochem. / Purification of heparin binding growth factors by Lobb (1986)
{'key': '2023050311113154300_DEVELOP_105_1_147C15', 'first-page': '811', 'article-title': 'Chemical structure of the morphogen differentiation inducing factor from Dictyostelium discoideum. Nature, Land.', 'volume': '328', 'author': 'Morris', 'year': '1987'} / Chemical structure of the morphogen differentiation inducing factor from Dictyostelium discoideum. Nature, Land. by Morris (1987)
10.1007/BF00578701 / Wilhelm Roux’ Arch. EntwMech. Org. / The formation of the mesoderm in urodelan amphibians. I. Induction by the endoderm by Nieuwkoop (1969)
10.1126/science.3422517 / Science / Mesoderm induction in amphibians: The role of TGF-/J2-like factors by Rosa (1988)
10.1038/326197a0 / Nature, Lond. / Mesoderm induction in early Xenopus embryos by heparin-binding growth factors by Slack (1987)
10.1242/dev.103.3.581 / Development / Inductive effects of fibroblast growth factor and lithium ion on Xenopus blastula ectoderm by Slack (1988)
10.1242/dev.99.1.3 / Development / A mesoderm inducing factor is produced by a Xenopus cell line by Smith (1987)
10.1242/dev.103.3.591 / Development / Purification, partial characterisation and biological effects of the XTC mesoderminducing factor by Smith (1988)
10.1038/327625a0 / Nature, Lond. / Identification and spatial distribution of retinoids in the developing chick limb bud by Thaller (1987)

Dates

Type	When
Created	4 years, 4 months ago (April 23, 2021, 2:23 p.m.)
Deposited	2 years, 3 months ago (May 3, 2023, 7:13 a.m.)
Indexed	1 month, 1 week ago (July 27, 2025, 3:54 a.m.)
Issued	36 years, 8 months ago (Jan. 1, 1989)
Published	36 years, 8 months ago (Jan. 1, 1989)
Published Online	36 years, 8 months ago (Jan. 1, 1989)
Published Print	36 years, 8 months ago (Jan. 1, 1989)

Funders 0

None

BibTeX

@article{Slack_1989, title={Presence of basic fibroblast growth factor in the early Xenopus embryo}, volume={105}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.105.1.147}, DOI={10.1242/dev.105.1.147}, number={1}, journal={Development}, publisher={The Company of Biologists}, author={Slack, J. M. W. and Isaacs, H. V.}, year={1989}, month=jan, pages={147–153} }

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