Abstract
ABSTRACTHomozygous mutant curly tail mouse embryos developing spinal neural tube defects (NTD) exhibit a celltype-specific abnormality of cell proliferation that affects the gut endoderm and notochord but not the neuroepithelium. We suggested that spinal NTD in these embryos may result from the imbalance of cell proliferation rates between affected and unaffected cell types. In order to test this hypothesis, curly tail embryos were subjected to influences that retard growth in vivo and in vitro. The expectation was that growth of unaffected rapidly growing cell types would be reduced to a greater extent than affected slowly growing cell types, thus counteracting the genetically determined imbalance of cell proliferation rates and leading to normalization of spinal neurulation.Food deprivation of pregnant females for 48 h prior to the stage of posterior neuropore closure reduced the overall incidence of spinal NTD and almost completely prevented open spina bifida, the most severe form of spinal NTD in curly tail mice. Analysis of embryos earlier in gestation showed that growth retardation acts by reducing the incidence of delayed neuropore closure. Culture of embryos at 40-5°C for 15-23 h from day 1(1 of gestation, like food deprivation in vivo, also produced growth retardation and led to normalization of posterior neuropore closure. Labelling of embryos in vitro with [3H]thymidine for 1 h at the end of the culture period showed that the labelling index is reduced to a greater extent in the neuroepithelium than in other cell types in growth-retarded embryos compared with controls cultured at 38°C. Moreover, the incidence of cell death is increased in the neuroepithelium to a greater extent than in other cell types. These results are consistent with the hypothesis that spinal NTD in curly tail embryos result from a genetically determined growth imbalance between neuroepithelium and gut endoderm/notochord.
References
27
Referenced
49
{'key': '2023040617324859200_', 'first-page': '233', 'article-title': 'Comparison of the sensitivity of normal hematopoietic and transplanted lymphoma colony-forming cells to chemotherapeutic agents administered in vivo', 'volume': '3', 'author': 'Bruce', 'year': '1966', 'journal-title': 'J. natn. Cancer Inst.'}/ J. natn. Cancer Inst. / Comparison of the sensitivity of normal hematopoietic and transplanted lymphoma colony-forming cells to chemotherapeutic agents administered in vivo by Bruce (1966)10.1002/tera.1420170306/ Teratology / Abnormalities induced in cultured rat embryos by hyperthermia by Cockroft (1978){'key': '2023040617324859200_', 'first-page': '109', 'article-title': 'Interaction between inner cell mass and trophectoderm of the mouse blastocyst. I. A study of cellular proliferation', 'volume': '48', 'author': 'Copp', 'year': '1978', 'journal-title': 'J. Embryol. exp. Morph.'}/ J. Embryol. exp. Morph. / Interaction between inner cell mass and trophectoderm of the mouse blastocyst. I. A study of cellular proliferation by Copp (1978){'key': '2023040617324859200_', 'first-page': '39', 'article-title': 'Relationship between timing of posterior neuropore closure and development of spinal neural tube defects in mutant (curly tail) and normal mouse embryos in culture', 'volume': '88', 'author': 'Copp', 'year': '1985', 'journal-title': 'J. Embryol. exp. Morph.'}/ J. Embryol. exp. Morph. / Relationship between timing of posterior neuropore closure and development of spinal neural tube defects in mutant (curly tail) and normal mouse embryos in culture by Copp (1985)10.1242/dev.104.2.285/ Development / A cell type-specific abnormality of cell proliferation in mutant (curly tail) mouse embryos developing spinal neural tube defects by Copp (1988){'key': '2023040617324859200_', 'article-title': 'Pathogenesis and prevention of spinal neural tube defects: evidence from a mouse model system', 'author': 'Copp', 'year': '1988', 'journal-title': 'N. Engl. J. Med. (Submitted)'}/ N. Engl. J. Med. (Submitted) / Pathogenesis and prevention of spinal neural tube defects: evidence from a mouse model system by Copp (1988){'key': '2023040617324859200_', 'first-page': '151', 'article-title': 'Neural tube development in mutant (curly tail) and normal mouse embryos: the timing of posterior neuropore closure in vivo and in vitro', 'volume': '69', 'author': 'Copp', 'year': '1982', 'journal-title': 'J. Embryol. exp. Morph.'}/ J. Embryol. exp. Morph. / Neural tube development in mutant (curly tail) and normal mouse embryos: the timing of posterior neuropore closure in vivo and in vitro by Copp (1982){'key': '2023040617324859200_', 'first-page': '662', 'article-title': 'Congenital malformations in twins', 'volume': '22', 'author': 'Hay', 'year': '1970', 'journal-title': 'Am. J. hum. Genet.'}/ Am. J. hum. Genet. / Congenital malformations in twins by Hay (1970)10.1002/tera.1420210103/ Teratology / Cell cycle phase specificity of hydroxyurea and its effects on the cell kinetics in embryonic spinal cord by Herken (1980)10.1002/ar.1092030308/ Anat. Rec. / Cephalic neurulation in the mouse embryo analyzed by SEM and morphometry by Jacobson (1982){'volume-title': 'Teratology of the Central Nervous Svstem.', 'year': '1968', 'author': 'Kalter', 'key': '2023040617324859200_'}/ Teratology of the Central Nervous Svstem. by Kalter (1968)10.1002/tera.1420320112/ Teratology / Geneteratogen interaction and its morphological basis in retinoic acid-induced mouse spina bifida by Kapron-Bras (1985)10.1016/0003-2697(80)90165-7/ Anal. Biochem. / A simple, rapid, and sensitive DNA assay procedure by Labarca (1980)10.1002/cne.901310103/ J. comp. Neurol. / Excess vitamin A and development of the cerebral cortex by Langman (1967){'key': '2023040617324859200_', 'first-page': '69', 'article-title': 'Congenital malformations in twins', 'volume': '32', 'author': 'Layde', 'year': '1980', 'journal-title': 'Am. J. hum. Genet.'}/ Am. J. hum. Genet. / Congenital malformations in twins by Layde (1980)10.1016/S0021-9258(19)52451-6/ J. biol. Chem. / Protein measurements with the folin phenol reagent by Lowry (1951)10.1002/tera.1420340308/ Teratology / Prevention of polydactyly manifestation in polydactyly Nagoya (pdn) mice by administration of cytosine arabinoside during pregnancy by Naruse (1986)10.1530/jrf.0.0350135/ J. Reprod. Fertil. / Culture of explanted rat embryos in rotating tubes by New (1973){'key': '2023040617324859200_', 'first-page': '63', 'article-title': 'Intrauterine growth in twin pregnancies: prediction of fetal growth retardation', 'volume': '66', 'author': 'Secher', 'year': '1985', 'journal-title': 'Obstet. Gynecol.'}/ Obstet. Gynecol. / Intrauterine growth in twin pregnancies: prediction of fetal growth retardation by Secher (1985){'key': '2023040617324859200_', 'first-page': '95', 'article-title': 'Neural tube defects in curly-tail mice. n. Effect of maternal administration of vitamin A', 'volume': 'B 206', 'author': 'Seller', 'year': '1979', 'journal-title': 'Proc. R. Soc. Land.'}/ Proc. R. Soc. Land. / Neural tube defects in curly-tail mice. n. Effect of maternal administration of vitamin A by Seller (1979){'key': '2023040617324859200_', 'first-page': '11', 'article-title': 'Effect of hydroxyurea on neural tube defects in the curly-tail mouse', 'volume': '3', 'author': 'Seller', 'year': '1983', 'journal-title': 'J. Craniofac. Genet, dev. Biol.'}/ J. Craniofac. Genet, dev. Biol. / Effect of hydroxyurea on neural tube defects in the curly-tail mouse by Seller (1983)10.1002/tera.1420330308/ Teratology / Effect of mitomycin C on the neural tube defects of the curly-tail mouse by Seller (1986)10.1016/S0140-6736(83)92654-5/ Lancet i, 1027-1031 / Further experience of vitamin supplementation for prevention of neural tube defect recurrences by Smtthells (1983)10.1136/adc.56.12.911/ Archs Dis. Childh. / Apparent prevention of neural tube defects by periconceptional vitamin supplementation by Smithells (1981)10.1038/279555a0/ Nature, Land. / Is compensatory growth a complicating factor in mouse teratology? by Snow (1979)10.1016/S0140-6736(82)91571-9/ Lancet / Does growth retardation predispose the fetus to congenital malformation? by Spiers (1982){'key': '2023040617324859200_', 'first-page': '988', 'article-title': 'Neural tube defects among twin births', 'volume': '34', 'author': 'Windham', 'year': '1982', 'journal-title': 'Am. J. hum. Genet.'}/ Am. J. hum. Genet. / Neural tube defects among twin births by Windham (1982)
Dates
| Type | When |
|---|---|
| Created | 4 years, 4 months ago (April 23, 2021, 2:22 p.m.) |
| Deposited | 1 year ago (Aug. 29, 2024, 3:09 a.m.) |
| Indexed | 3 months, 1 week ago (May 20, 2025, 10:34 a.m.) |
| Issued | 36 years, 10 months ago (Oct. 1, 1988) |
| Published | 36 years, 10 months ago (Oct. 1, 1988) |
| Published Online | 36 years, 10 months ago (Oct. 1, 1988) |
| Published Print | 36 years, 10 months ago (Oct. 1, 1988) |
@article{Copp_1988, title={Prevention of spinal neural tube defects in the mouse embryo by growth retardation during neurulation}, volume={104}, ISSN={1477-9129}, url={http://dx.doi.org/10.1242/dev.104.2.297}, DOI={10.1242/dev.104.2.297}, number={2}, journal={Development}, publisher={The Company of Biologists}, author={Copp, Andrew J. and Crolla, John A. and Brook, Frances A.}, year={1988}, month=oct, pages={297–303} }