C. elegans sperm bud vesicles to deliver a meiotic maturation signal to distant oocytes
10.1242/dev.01916
Crossref journal-article
The Company of Biologists
Development (237)
Abstract

The major sperm protein (MSP) is the central cytoskeletal element required for actin-independent motility of nematode spermatozoa. MSP has a dual role in Caenorhabditis elegans reproduction, functioning as a hormone for both oocyte meiotic maturation and ovarian muscle contraction. The identification of the signaling function of MSP raised the question, how do spermatozoa, which are devoid of ribosomes, ER and Golgi, release a cytoplasmic protein lacking a signal sequence? Here, we provide evidence that MSP export occurs by the budding of novel vesicles that have both inner and outer membranes with MSP sandwiched in between. MSP vesicles are apparently labile structures that generate long-range MSP gradients for signaling at the oocyte cell surface. Both spermatozoa and non-motile spermatids bud MSP vesicles, but their stability and signaling properties differ. Budding protrusions from the cell body contain MSP, but not the MSD proteins, which counteract MSP filament assembly. We propose that MSP generates the protrusive force for its own vesicular export.

Bibliography

Kosinski, M., McDonald, K., Schwartz, J., Yamamoto, I., & Greenstein, D. (2005). C. elegans sperm bud vesicles to deliver a meiotic maturation signal to distant oocytes. Development, 132(15), 3357–3369.

Authors 5
  1. Mary Kosinski (first)
  2. Kent McDonald (additional)
  3. Joel Schwartz (additional)
  4. Ikuko Yamamoto (additional)
  5. David Greenstein (additional)
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Dates
Type When
Created 20 years, 2 months ago (June 23, 2005, 8:15 p.m.)
Deposited 4 years, 4 months ago (April 26, 2021, 12:36 p.m.)
Indexed 1 month, 1 week ago (July 20, 2025, 12:08 a.m.)
Issued 20 years ago (Aug. 1, 2005)
Published 20 years ago (Aug. 1, 2005)
Published Print 20 years ago (Aug. 1, 2005)
Funders 0

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@article{Kosinski_2005, title={C. elegans sperm bud vesicles to deliver a meiotic maturation signal to distant oocytes}, volume={132}, ISSN={0950-1991}, url={http://dx.doi.org/10.1242/dev.01916}, DOI={10.1242/dev.01916}, number={15}, journal={Development}, publisher={The Company of Biologists}, author={Kosinski, Mary and McDonald, Kent and Schwartz, Joel and Yamamoto, Ikuko and Greenstein, David}, year={2005}, month=aug, pages={3357–3369} }