DOI: 10.1242/dev.01782. The homeodomain protein PAL-1 specifies a lineage-specific regulatory network in the<i>C. elegans</i>embryo

The homeodomain protein PAL-1 specifies a lineage-specific regulatory network in theC. elegansembryo

10.1242/dev.01782

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

Maternal and zygotic activities of the homeodomain protein PAL-1 specify the identity and maintain the development of the multipotent C blastomere lineage in the C. elegans embryo. To identify PAL-1 regulatory target genes, we used microarrays to compare transcript abundance in wild-type embryos with mutant embryos lacking a C blastomere and to mutant embryos with extra C blastomeres. pal-1-dependent C-lineage expression was verified for select candidate target genes by reporter gene analysis, though many of the target genes are expressed in additional lineages as well. The set of validated target genes includes 12 transcription factors, an uncharacterized wingless ligand and five uncharacterized genes. Phenotypic analysis demonstrates that the identified PAL-1 target genes affect specification, differentiation and morphogenesis of C-lineage cells. In particular, we show that cell fate-specific genes (or tissue identity genes)and a posterior HOX gene are activated in lineage-specific fashion. Transcription of targets is initiated in four temporal phases, which together with their spatial expression patterns leads to a model of the regulatory network specified by PAL-1.

Bibliography

Baugh, L. R., Hill, A. A., Claggett, J. M., Hill-Harfe, K., Wen, J. C., Slonim, D. K., Brown, E. L., & Hunter, C. P. (2005). The homeodomain protein PAL-1 specifies a lineage-specific regulatory network in theC. elegansembryo. Development, 132(8), 1843â1854.

Authors 8

L. Ryan Baugh (first)
Andrew A. Hill (additional)
Julia M. Claggett (additional)
Kate Hill-Harfe (additional)
Joanne C. Wen (additional)
Donna K. Slonim (additional)
Eugene L. Brown (additional)
Craig P. Hunter (additional)

References 52 Referenced 103

Dates

Type	When
Created	20 years, 5 months ago (March 16, 2005, 8:14 p.m.)
Deposited	1 year, 7 months ago (Jan. 23, 2024, 10:02 p.m.)
Indexed	3 weeks, 2 days ago (Aug. 6, 2025, 8:42 a.m.)
Issued	20 years, 4 months ago (April 15, 2005)
Published	20 years, 4 months ago (April 15, 2005)
Published Print	20 years, 4 months ago (April 15, 2005)

Funders 0

None

BibTeX

@article{Baugh_2005, title={The homeodomain protein PAL-1 specifies a lineage-specific regulatory network in theC. elegansembryo}, volume={132}, ISSN={0950-1991}, url={http://dx.doi.org/10.1242/dev.01782}, DOI={10.1242/dev.01782}, number={8}, journal={Development}, publisher={The Company of Biologists}, author={Baugh, L. Ryan and Hill, Andrew A. and Claggett, Julia M. and Hill-Harfe, Kate and Wen, Joanne C. and Slonim, Donna K. and Brown, Eugene L. and Hunter, Craig P.}, year={2005}, month=apr, pages={1843–1854} }

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