DOI: 10.1242/dev.000885. The FERM protein Epb4.1l5 is required for organization of the neural plate and for the epithelial-mesenchymal transition at the primitive streak of the mouse embryo

The FERM protein Epb4.1l5 is required for organization of the neural plate and for the epithelial-mesenchymal transition at the primitive streak of the mouse embryo

10.1242/dev.000885

Crossref journal-article

The Company of Biologists

Development (237)

Abstract

During early mouse development, a single-layered epithelium is transformed into the three germ layers that are the basis of the embryonic body plan. Here we describe an ENU-induced mutation, limulus (lulu), which disrupts gastrulation and the organization of all three embryonic germ layers. Positional cloning and analysis of additional alleles show that luluis a null allele of the FERM-domain gene erythrocyte protein band 4.1-like 5(Epb4.1l5). During gastrulation, some cells in lulu mutants are trapped in the primitive streak at an intermediate stage of the epithelial-mesenchymal transition; as a result, the embryos have very little paraxial mesoderm. Epithelial layers of the later lulu embryo are also disrupted: definitive endoderm is specified but does not form a gut tube,and the neural plate is broad and forms ectopic folds rather than closing to make the neural tube. In contrast to zebrafish and Drosophila, in which orthologs of Epb4.1l5 control the apical localization and activity of Crumbs proteins, mouse Crumbs proteins are localized normally to the apical surface of the lulu mutant epiblast and neural plate. However, the defects in both the lulu primitive streak and neural plate are associated with disruption of the normal organization of the actin cytoskeleton. We propose that mouse Lulu (Epb4.1l5) helps anchor the actin-myosin contractile machinery to the membrane to allow the dynamic rearrangements of epithelia that mediate embryonic morphogenesis.

Bibliography

Lee, J. D., Silva-Gagliardi, N. F., Tepass, U., McGlade, C. J., & Anderson, K. V. (2007). The FERM protein Epb4.1l5 is required for organization of the neural plate and for the epithelial-mesenchymal transition at the primitive streak of the mouse embryo. Development, 134(11), 2007â2016.

Authors 5

Jeffrey D. Lee (first)
Nancy F. Silva-Gagliardi (additional)
Ulrich Tepass (additional)
C. Jane McGlade (additional)
Kathryn V. Anderson (additional)

References 43 Referenced 71

Dates

Type	When
Created	18 years, 3 months ago (May 16, 2007, 1:29 p.m.)
Deposited	2 years, 3 months ago (May 11, 2023, 6 p.m.)
Indexed	3 months ago (May 26, 2025, 1:05 p.m.)
Issued	18 years, 2 months ago (June 1, 2007)
Published	18 years, 2 months ago (June 1, 2007)
Published Print	18 years, 2 months ago (June 1, 2007)

Funders 0

None

BibTeX

@article{Lee_2007, title={The FERM protein Epb4.1l5 is required for organization of the neural plate and for the epithelial-mesenchymal transition at the primitive streak of the mouse embryo}, volume={134}, ISSN={0950-1991}, url={http://dx.doi.org/10.1242/dev.000885}, DOI={10.1242/dev.000885}, number={11}, journal={Development}, publisher={The Company of Biologists}, author={Lee, Jeffrey D. and Silva-Gagliardi, Nancy F. and Tepass, Ulrich and McGlade, C. Jane and Anderson, Kathryn V.}, year={2007}, month=jun, pages={2007–2016} }

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  "abstract": "<jats:p>During early mouse development, a single-layered epithelium is transformed into the three germ layers that are the basis of the embryonic body plan. Here we describe an ENU-induced mutation, limulus (lulu), which disrupts gastrulation and the organization of all three embryonic germ layers. Positional cloning and analysis of additional alleles show that luluis a null allele of the FERM-domain gene erythrocyte protein band 4.1-like 5(Epb4.1l5). During gastrulation, some cells in lulu mutants are trapped in the primitive streak at an intermediate stage of the epithelial-mesenchymal transition; as a result, the embryos have very little paraxial mesoderm. Epithelial layers of the later lulu embryo are also disrupted: definitive endoderm is specified but does not form a gut tube,and the neural plate is broad and forms ectopic folds rather than closing to make the neural tube. In contrast to zebrafish and Drosophila, in which orthologs of Epb4.1l5 control the apical localization and activity of Crumbs proteins, mouse Crumbs proteins are localized normally to the apical surface of the lulu mutant epiblast and neural plate. However, the defects in both the lulu primitive streak and neural plate are associated with disruption of the normal organization of the actin cytoskeleton. We propose that mouse Lulu (Epb4.1l5) helps anchor the actin-myosin contractile machinery to the membrane to allow the dynamic rearrangements of epithelia that mediate embryonic morphogenesis.</jats:p>",
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