DOI: 10.1210/endo.138.4.5052. Integrin Binding to Immobilized Collagen and Fibronectin Stimulates the Proliferation of Human Thyroid Cells in Culture*

Integrin Binding to Immobilized Collagen and Fibronectin Stimulates the Proliferation of Human Thyroid Cells in Culture*

10.1210/endo.138.4.5052

Crossref journal-article

The Endocrine Society

Endocrinology (80)

Abstract

AbstractThe expression of integrins of the β1 family and their possible biological effects were investigated in normal human thyroid cells in monolayer culture. The expression of β1 andα 1–6 integrin subunits was determined by flow cytofluorometry with specific antibodies. Follicular cells of subconfluent monolayer cultures expressedα 2β1 and α3β1 at high levels, while α1β1 was only slightly expressed, and α4β1,α 5β1, and α6β1 were never detected. Cell attachment assays were performed in fibronectin-, type I collagen-, and laminin-coated microtiter plates. Thyroid cells, while adherent to collagen and fibronectin, showed poor attachment to laminin despite the abundance of their putative receptorsα 2β1 and α3β1. In serum-free medium, collagen and fibronectin induced cytoskeletal organization, change of cell shape from round to flat, and cell spreading. [3H]Thymidine incorporation and proliferation assays were used to evaluate the effects of collagen and fibronectin on DNA synthesis and cell growth in the absence of a change in spreading or cell shape. Both substrates, in low serum-containing medium, induced a concentration-dependent increase in [3H]thymidine incorporation partially inhibited by RGD-containing peptides that blocked the cell attachment.Thyrocytes cultured in low serum-containing medium on immobilized fibronectin or collagen showed a dose-dependent stimulation of proliferation. These data indicate that fibronectin and collagen can regulate the cytoskeletal organization and cell shape and stimulate the proliferation of normal human thyroid cells in culture and that integrins mediate these effects of extracellular matrix proteins.

Bibliography

Vitale, M., Illario, M., Di Matola, T., Casamassima, A., Fenzi, G., & Rossi, G. (1997). Integrin Binding to Immobilized Collagen and Fibronectin Stimulates the Proliferation of Human Thyroid Cells in Culture*. Endocrinology, 138(4), 1642â1648.

Authors 6

Mario Vitale (first)
Maddalena Illario (additional)
Tiziana Di Matola (additional)
Adele Casamassima (additional)
Gianfranco Fenzi (additional)
Guido Rossi (additional)

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Dates

Type	When
Created	11 years, 7 months ago (Jan. 8, 2014, 11:08 a.m.)
Deposited	2 years, 1 month ago (July 9, 2023, 10:37 a.m.)
Indexed	1 week, 3 days ago (Aug. 19, 2025, 6:41 a.m.)
Issued	28 years, 4 months ago (April 1, 1997)
Published	28 years, 4 months ago (April 1, 1997)
Published Print	28 years, 4 months ago (April 1, 1997)

Funders 0

None

BibTeX

@article{Vitale_1997, title={Integrin Binding to Immobilized Collagen and Fibronectin Stimulates the Proliferation of Human Thyroid Cells in Culture*}, volume={138}, ISSN={1945-7170}, url={http://dx.doi.org/10.1210/endo.138.4.5052}, DOI={10.1210/endo.138.4.5052}, number={4}, journal={Endocrinology}, publisher={The Endocrine Society}, author={Vitale, Mario and Illario, Maddalena and Di Matola, Tiziana and Casamassima, Adele and Fenzi, Gianfranco and Rossi, Guido}, year={1997}, month=apr, pages={1642–1648} }

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