DOI: 10.1189/jlb.68.3.366. HIV-1 Vpr regulates expression of β chemokines in human primary lymphocytes and macrophages [Penn]

HIV-1 Vpr regulates expression of β chemokines in human primary lymphocytes and macrophages

10.1189/jlb.68.3.366

Crossref journal-article

Oxford University Press (OUP)

Journal of Leukocyte Biology (286)

Abstract

Abstract The HIV-1 vpr gene encodes a 14-kDa virion-packaged protein that has been implicated in viral pathogenesis. Vpr exhibits profound effects on human primary cells influencing proliferation, differentiation, apoptosis, and cytokine production, in part through NF-κB-mediated transcription. NF-κB, a potent transcription factor, activates many proinflammatory cytokines/chemokines upon infection. Here, we analyzed the effect of extracellular Vpr as well as the virion-associated Vpr on β chemokines (MIP-1α, MIP-1β, and RANTES) production in human macrophages and primary lymphocytes (PBLs). Macrophages and PBLs exposed to HIV-1 vpr+ viruses or to recombinant Vpr protein produced significantly less β chemokines compared with cells infected with HIV-1 vpr− viruses or irrelevant control protein (Gag)-exposed cells. These results suggest that a Vpr-mediated increase in virus replication could be in part through down-regulation of chemokine production.

Bibliography

Muthumani, K., Kudchodkar, S., Papasavvas, E., Montaner, L. J., Weiner, D. B., & Ayyavoo, V. (2000). HIV-1 Vpr regulates expression of Î² chemokines in human primary lymphocytes and macrophages. Journal of Leukocyte Biology, 68(3), 366â372.

Authors 6

Karuppiah Muthumani (first)
Sagar Kudchodkar (additional)
Emmanouil Papasavvas (additional)
Luis J Montaner (additional)
David B Weiner (additional)
Velpandi Ayyavoo (additional)

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Dates

Type	When
Created	2 years, 11 months ago (Sept. 27, 2022, 9:46 p.m.)
Deposited	2 years, 5 months ago (March 30, 2023, 8:24 p.m.)
Indexed	3 months ago (June 3, 2025, 1 a.m.)
Issued	25 years ago (Sept. 1, 2000)
Published	25 years ago (Sept. 1, 2000)
Published Online	25 years ago (Sept. 1, 2000)
Published Print	25 years ago (Sept. 1, 2000)

Funders 1

National Institutes of Health 10.13039/100000002
Region: Americas
gov (National government)
Labels3
1. Institutos Nacionales de la Salud
2. US National Institutes of Health
3. NIH

BibTeX

@article{Muthumani_2000, title={HIV-1 Vpr regulates expression of β chemokines in human primary lymphocytes and macrophages}, volume={68}, ISSN={0741-5400}, url={http://dx.doi.org/10.1189/jlb.68.3.366}, DOI={10.1189/jlb.68.3.366}, number={3}, journal={Journal of Leukocyte Biology}, publisher={Oxford University Press (OUP)}, author={Muthumani, Karuppiah and Kudchodkar, Sagar and Papasavvas, Emmanouil and Montaner, Luis J and Weiner, David B and Ayyavoo, Velpandi}, year={2000}, month=sep, pages={366–372} }

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