DOI: 10.1189/jlb.0804473. IL-4 supports the generation of a dendritic cell subset from murine bone marrow with altered endocytosis capacity

IL-4 supports the generation of a dendritic cell subset from murine bone marrow with altered endocytosis capacity

10.1189/jlb.0804473

Crossref journal-article

Oxford University Press (OUP)

Journal of Leukocyte Biology (286)

Abstract

AbstractDendritic cells (DC) of myeloid origin can be generated from mouse bone marrow (BM) using granulocyte macrophage-colony stimulating factor (GM-CSF). Immature major histocompatibility complex (MHC) IIlow DC are known to bear a high endocytosis capacity, in contrast to DC precursors and mature DC. Now we found that a subset of MHC IIlow DC in BM-DC cultures is unable to exert mannose receptor-mediated endocytosis of fluorescein isothiocyanate (FITC)-dextran (DX) and resembles immature Langerhans cells (LC). The FITC-DX endocytosis activity of LC-like cells occurs at an earlier stage of development, where the surface MHC II expression is absent or very weak. This LC-like subset expresses higher levels of E-cadherin but lower amounts of the markers Gr-1, scavenger receptor 2F8, and CD11b, when compared with the highly endocytic DC subset. The latter myeloid DC resemble monocyte-derived DC (MoDC). The sorted LC-like population develops completely and exclusively into mature MHC IIhigh DC, and the MoDC-like cells remain immature MHC IIlow DC or develop into adherent MHC IIneg macrophages or mature into MHC IIhigh DC. The development of LC-like cells is promoted by interleukin-4. Thus, we show here that the simultaneous development of LC-like and MoDC-like DC subsets occurs in standard bulk cultures with GM-CSF, suggesting the existence of two different precursors for LC and MoDC in BM.

Bibliography

Menges, M., Baumeister, T., RÃ¶ssner, S., Stoitzner, P., Romani, N., Gessner, A., & Lutz, M. B. (2004). IL-4 supports the generation of a dendritic cell subset from murine bone marrow with altered endocytosis capacity. Journal of Leukocyte Biology, 77(4), 535â543.

Authors 7

Mauritius Menges (first)
Thomas Baumeister (additional)
Susanne Rössner (additional)
Patrizia Stoitzner (additional)
Nikolaus Romani (additional)
André Gessner (additional)
Manfred B Lutz (additional)

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Dates

Type	When
Created	20 years, 8 months ago (Dec. 23, 2004, 9:30 p.m.)
Deposited	2 years, 4 months ago (April 30, 2023, 10:43 p.m.)
Indexed	4 months, 2 weeks ago (April 19, 2025, 12:25 a.m.)
Issued	20 years, 8 months ago (Dec. 23, 2004)
Published	20 years, 8 months ago (Dec. 23, 2004)
Published Online	20 years, 8 months ago (Dec. 23, 2004)
Published Print	20 years, 8 months ago (Dec. 23, 2004)

Funders 2

Deutsche Forschungsgemeinschaft 10.13039/501100001659
Region: Europe
gov (National government)
Labels3
1. German Research Association
2. German Research Foundation
3. DFG
Awards3
1. SFB643
2. Lu 851/2-1
3. Lu 851/1-1
ELAN Fonds of the University Hospital Erlangen

BibTeX

@article{Menges_2004, title={IL-4 supports the generation of a dendritic cell subset from murine bone marrow with altered endocytosis capacity}, volume={77}, ISSN={1938-3673}, url={http://dx.doi.org/10.1189/jlb.0804473}, DOI={10.1189/jlb.0804473}, number={4}, journal={Journal of Leukocyte Biology}, publisher={Oxford University Press (OUP)}, author={Menges, Mauritius and Baumeister, Thomas and Rössner, Susanne and Stoitzner, Patrizia and Romani, Nikolaus and Gessner, André and Lutz, Manfred B}, year={2004}, month=dec, pages={535–543} }

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