Identification of a functional docking site in the Rpn1 LRR domain for the UBA-UBL domain protein Ddi1
10.1186/1741-7007-9-33
Crossref journal-article
Springer Science and Business Media LLC
BMC Biology (297)
Abstract

Abstract Background The proteasome is a multi-subunit protein machine that is the final destination for cellular proteins that have been marked for degradation via an ubiquitin (Ub) chain appendage. These ubiquitylated proteins either bind directly to the intrinsic proteasome ubiqutin chain receptors Rpn10, Rpn13, or Rpt5, or are shuttled to the proteasome by Rad23, Dsk2, or Ddi1. The latter proteins share an Ub association domain (UBA) for binding poly-Ub chains and an Ub-like-domain (UBL) for binding to the proteasome. It has been proposed that shuttling receptors dock on the proteasome via Rpn1, but the precise nature of the docking site remains poorly defined. Results To shed light on the recruitment of shuttling receptors to the proteasome, we performed both site-directed mutagenesis and genetic screening to identify mutations in Rpn1 that disrupt its binding to UBA-UBL proteins. Here we demonstrate that delivery of Ub conjugates and docking of Ddi1 (and to a lesser extent Dsk2) to the proteasome are strongly impaired by an aspartic acid to alanine point mutation in the highly-conserved D517 residue of Rpn1. Moreover, degradation of the Ddi1-dependent proteasome substrate, Ufo1, is blocked in rpn1-D517A yeast cells. By contrast, Rad23 recruitment to the proteasome is not affected by rpn1-D517A. Conclusions These studies provide insight into the mechanism by which the UBA-UBL protein Ddi1 is recruited to the proteasome to enable Ub-dependent degradation of its ligands. Our studies suggest that different UBA-UBL proteins are recruited to the proteasome by distinct mechanisms.

Bibliography

Gomez, T. A., Kolawa, N., Gee, M., Sweredoski, M. J., & Deshaies, R. J. (2011). Identification of a functional docking site in the Rpn1 LRR domain for the UBA-UBL domain protein Ddi1. BMC Biology, 9(1).

Authors 5
  1. Tara A Gomez (first)
  2. Natalie Kolawa (additional)
  3. Marvin Gee (additional)
  4. Michael J Sweredoski (additional)
  5. Raymond J Deshaies (additional)
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Dates
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Created 14 years, 2 months ago (May 31, 2011, 2:24 p.m.)
Deposited 3 years, 11 months ago (Sept. 1, 2021, 10:12 a.m.)
Indexed 2 months, 2 weeks ago (June 6, 2025, 2:05 a.m.)
Issued 14 years, 2 months ago (May 31, 2011)
Published 14 years, 2 months ago (May 31, 2011)
Published Online 14 years, 2 months ago (May 31, 2011)
Published Print 13 years, 8 months ago (Dec. 1, 2011)
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@article{Gomez_2011, title={Identification of a functional docking site in the Rpn1 LRR domain for the UBA-UBL domain protein Ddi1}, volume={9}, ISSN={1741-7007}, url={http://dx.doi.org/10.1186/1741-7007-9-33}, DOI={10.1186/1741-7007-9-33}, number={1}, journal={BMC Biology}, publisher={Springer Science and Business Media LLC}, author={Gomez, Tara A and Kolawa, Natalie and Gee, Marvin and Sweredoski, Michael J and Deshaies, Raymond J}, year={2011}, month=may }