Comparative genomics profiling of clinical isolates of Aeromonas salmonicida using DNA microarrays
10.1186/1471-2164-7-43
Crossref journal-article
Springer Science and Business Media LLC
BMC Genomics (297)
Abstract

Abstract Background Aeromonas salmonicida has been isolated from numerous fish species and shows wide variation in virulence and pathogenicity. As part of a larger research program to identify virulence genes and candidates for vaccine development, a DNA microarray was constructed using a subset of 2024 genes from the draft genome sequence of A. salmonicida subsp. salmonicida strain A449. The microarray included genes encoding known virulence-associated factors in A. salmonicida and homologs of virulence genes of other pathogens. We used microarray-based comparative genomic hybridizations (M-CGH) to compare selected A. salmonicida sub-species and other Aeromonas species from different hosts and geographic locations. Results Results showed variable carriage of virulence-associated genes and generally increased variation in gene content across sub-species and species boundaries. The greatest variation was observed among genes associated with plasmids and transposons. There was little correlation between geographic region and degree of variation for all isolates tested. Conclusion We have used the M-CGH technique to identify subsets of conserved genes from amongst this set of A. salmonicida virulence genes for further investigation as potential vaccine candidates. Unlike other bacterial characterization methods that use a small number of gene or DNA-based functions, M-CGH examines thousands of genes and/or whole genomes and thus is a more comprehensive analytical tool for veterinary or even human health research.

Bibliography

Nash, J. H., Findlay, W. A., Luebbert, C. C., Mykytczuk, O. L., Foote, S. J., Taboada, E. N., Carrillo, C. D., Boyd, J. M., Colquhoun, D. J., Reith, M. E., & Brown, L. L. (2006). Comparative genomics profiling of clinical isolates of Aeromonas salmonicida using DNA microarrays. BMC Genomics, 7(1).

Authors 11
  1. John HE Nash (first)
  2. Wendy A Findlay (additional)
  3. Christian C Luebbert (additional)
  4. Oksana L Mykytczuk (additional)
  5. Simon J Foote (additional)
  6. Eduardo N Taboada (additional)
  7. Catherine D Carrillo (additional)
  8. Jessica M Boyd (additional)
  9. Duncan J Colquhoun (additional)
  10. Michael E Reith (additional)
  11. Laura L Brown (additional)
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Dates
Type When
Created 19 years, 5 months ago (March 10, 2006, 2:18 p.m.)
Deposited 4 years ago (Aug. 31, 2021, 9:40 p.m.)
Indexed 3 months, 2 weeks ago (May 20, 2025, 9:15 a.m.)
Issued 19 years, 5 months ago (March 7, 2006)
Published 19 years, 5 months ago (March 7, 2006)
Published Online 19 years, 5 months ago (March 7, 2006)
Published Print 18 years, 9 months ago (Dec. 1, 2006)
Funders 0

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@article{Nash_2006, title={Comparative genomics profiling of clinical isolates of Aeromonas salmonicida using DNA microarrays}, volume={7}, ISSN={1471-2164}, url={http://dx.doi.org/10.1186/1471-2164-7-43}, DOI={10.1186/1471-2164-7-43}, number={1}, journal={BMC Genomics}, publisher={Springer Science and Business Media LLC}, author={Nash, John HE and Findlay, Wendy A and Luebbert, Christian C and Mykytczuk, Oksana L and Foote, Simon J and Taboada, Eduardo N and Carrillo, Catherine D and Boyd, Jessica M and Colquhoun, Duncan J and Reith, Michael E and Brown, Laura L}, year={2006}, month=mar }