Formulation of novel lipid-coated magnetic nanoparticles as the probe for in vivo imaging
10.1186/1423-0127-16-86
Crossref journal-article
Springer Science and Business Media LLC
Journal of Biomedical Science (297)
Abstract

AbstractBackgroundApplication of superparamagnetic iron oxide nanoparticles (SPIOs) as the contrast agent has improved the quality of magnetic resonance (MR) imaging. Low efficiency of loading the commercially available iron oxide nanoparticles into cells and the cytotoxicity of previously formulated complexes limit their usage as the image probe. Here, we formulated new cationic lipid nanoparticles containing SPIOs feasible forin vivoimaging.MethodsHydrophobic SPIOs were incorporated into cationic lipid 1,2-dioleoyl-3-(trimethylammonium) propane (DOTAP) and polyethylene-glycol-2000-1,2-distearyl-3-sn-phosphatidylethanolamine (PEG-DSPE) based micelles by self-assembly procedure to form lipid-coated SPIOs (L-SPIOs). Trace amount of Rhodamine-dioleoyl-phosphatidylethanolamine (Rhodamine-DOPE) was added as a fluorescent indicator. Particle size and zeta potential of L-SPIOs were determined by Dynamic Light Scattering (DLS) and Laser Doppler Velocimetry (LDV), respectively. HeLa, PC-3 and Neuro-2a cells were tested for loading efficiency and cytotoxicity of L-SPIOs using fluorescent microscopy, Prussian blue staining and flow cytometry. L-SPIO-loaded CT-26 cells were tested forin vivoMR imaging.ResultsThe novel formulation generates L-SPIOs particle with the average size of 46 nm. We showed efficient cellular uptake of these L-SPIOs with cationic surface charge into HeLa, PC-3 and Neuro-2a cells. The L-SPIO-loaded cells exhibited similar growth potential as compared to unloaded cells, and could be sorted by a magnet stand over ten-day duration. Furthermore, when SPIO-loaded CT-26 tumor cells were injected into Balb/c mice, the growth status of these tumor cells could be monitored using optical and MR images.ConclusionWe have developed a novel cationic lipid-based nanoparticle of SPIOs with high loading efficiency, low cytotoxicity and long-term imaging signals. The results suggested these newly formulated non-toxic lipid-coated magnetic nanoparticles as a versatile image probe for cell tracking.

Bibliography

Huang, H.-C., Chang, P.-Y., Chang, K., Chen, C.-Y., Lin, C.-W., Chen, J.-H., Mou, C.-Y., Chang, Z.-F., & Chang, F.-H. (2009). Formulation of novel lipid-coated magnetic nanoparticles as the probe for in vivo imaging. Journal of Biomedical Science, 16(1).

Authors 9
  1. Huey-Chung Huang (first)
  2. Po-Yuan Chang (additional)
  3. Karen Chang (additional)
  4. Chao-Yu Chen (additional)
  5. Chung-Wu Lin (additional)
  6. Jyh-Horng Chen (additional)
  7. Chung-Yuan Mou (additional)
  8. Zee-Fen Chang (additional)
  9. Fu-Hsiung Chang (additional)
References 38 Referenced 53
  1. Grodzinski P, Silver M, Molnar LK: Nanotechnology for cancer diagnostics: promises and challenges. Expert Rev Mol Diagn. 2006, 6: 307-318. 10.1586/14737159.6.3.307. (10.1586/14737159.6.3.307) / Expert Rev Mol Diagn by P Grodzinski (2006)
  2. Gupta AK, Gupta M: Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials. 2005, 26: 3995-4021. 10.1016/j.biomaterials.2004.10.012. (10.1016/j.biomaterials.2004.10.012) / Biomaterials by AK Gupta (2005)
  3. Norman AB, Thomas SR, Pratt RG, Lu SY, Norgren RB: Magnetic resonance imaging of neural transplants in rat brain using a superparamagnetic contrast agent. Brain Res. 1992, 594: 279-283. 10.1016/0006-8993(92)91135-2. (10.1016/0006-8993(92)91135-2) / Brain Res by AB Norman (1992)
  4. Hawrylak N, Ghosh P, Broadus J, Schlueter C, Greenough WT, Lauterbur PC: Nuclear magnetic resonance (NMR) imaging of iron oxide-labeled neural transplants. Exp Neurol. 1993, 121: 181-92. 10.1006/exnr.1993.1085. (10.1006/exnr.1993.1085) / Exp Neurol by N Hawrylak (1993)
  5. Flexman JA, Minoshima S, Kim Y, Cross DJ: Magneto-optical labeling of fetal neural stem cells for in vivo MRI tracking. Conf Proc IEEE Eng Med Biol Soc. 2006, 1: 5631-5634. full_text. (10.1109/IEMBS.2006.259982) / Conf Proc IEEE Eng Med Biol Soc by JA Flexman (2006)
  6. Lewin M, Carlesso N, Tung CH, Tang XW, Cory D, Scadden DT, Weissleder R: Tat peptide-derivatized magnetic nanoparticles allow in vivo tracking and recovery of progenitor cells. Nat Biotechnol. 2000, 18: 410-414. 10.1038/74464. (10.1038/74464) / Nat Biotechnol by M Lewin (2000)
  7. Bulte JW, Douglas T, Witwer B, Zhang SC, Strable E, Lewis BK, Zywicke H, Miller B, van Gelderen P, Moskowitz BM, Duncan ID, Frank JA: Magnetodendrimers allow endosomal magnetic labeling and in vivo tracking of stem cells. Nat Biotechnol. 2001, 19: 1141-1147. 10.1038/nbt1201-1141. (10.1038/nbt1201-1141) / Nat Biotechnol by JW Bulte (2001)
  8. Hoehn M, Küstermann E, Blunk J, Wiedermann D, Trapp T, Wecker S, Föcking M, Arnold H, Hescheler J, Fleischmann BK, Schwindt W, Bührle C: Monitoring of implanted stem cell migration in vivo: a highly resolved in vivo magnetic resonance imaging investigation of experimental stroke in rat. Proc Natl Acad Sci USA. 2002, 99: 16267-16272. 10.1073/pnas.242435499. (10.1073/pnas.242435499) / Proc Natl Acad Sci USA by M Hoehn (2002)
  9. Kircher MF, Allport JR, Graves EE, Love V, Josephson L, Lichtman AH, Weissleder R: In vivo high resolution three-dimensional imaging of antigen-specific cytotoxic T-lymphocyte trafficking to tumors. Cancer Res. 2003, 63: 6838-6846. / Cancer Res by MF Kircher (2003)
  10. Arbab AS, Yocum GT, Wilson LB, Parwana A, Jordan EK, Kalish H, Frank JA: Comparison of transfection agents in forming complexes with ferumoxides, cell labeling efficiency, and cellular viability. Mol Imaging. 2004, 3: 24-32. 10.1162/153535004773861697. (10.1162/153535004773861697) / Mol Imaging by AS Arbab (2004)
  11. Berger C, Rausch M, Schmidt P, Rudin M: Feasibility and limits of magnetically labeling primary cultured rat T cells with ferumoxides coupled with commonly used transfection agents. Mol Imaging. 2006, 5: 93-104. (10.2310/7290.2006.00010) / Mol Imaging by C Berger (2006)
  12. Matuszewski L, Persigehl T, Wall A, Schwindt W, Tombach B, Fobker M, Poremba C, Ebert W, Heindel W, Bremer C: Cell tagging with clinically approved iron oxides: feasibility and effect of lipofection, particle size, and surface coating on labeling efficiency. Radiology. 2005, 235: 155-161. 10.1148/radiol.2351040094. (10.1148/radiol.2351040094) / Radiology by L Matuszewski (2005)
  13. Miyoshi S, Flexman JA, Cross DJ, Maravilla KR, Kim Y, Anzai Y, Oshima J, Minoshima S: Transfection of neuroprogenitor cells with iron nanoparticles for magnetic resonance imaging tracking: cell viability, differentiation, and intracellular localization. Mol Imaging Biol. 2005, 7: 286-295. 10.1007/s11307-005-0008-1. (10.1007/s11307-005-0008-1) / Mol Imaging Biol by S Miyoshi (2005)
  14. Montet-Abou K, Montet X, Weissleder R, Josephson L: Cell internalization of magnetic nanoparticles using transfection agents. Mol Imaging. 2007, 6: 1-9. (10.2310/7290.2006.00028) / Mol Imaging by K Montet-Abou (2007)
  15. Hong RL, Huang CJ, Tseng YL, Pang VF, Chen ST, Liu JJ, Chang FH: Direct comparison of liposomal doxorubicin with or without polyethylene glycol coating in C-26 tumor-bearing mice: is surface coating with polyethylene glycol beneficial?. Clin Cancer Res. 1999, 5: 3645-52. / Clin Cancer Res by RL Hong (1999)
  16. Torchilin V: Micellar nanocarriers: Pharmaceutical perspectives. Pharm Res. 2007, 24: 1-16. 10.1007/s11095-006-9132-0. (10.1007/s11095-006-9132-0) / Pharm Res by V Torchilin (2007)
  17. Simberg D, Danino D, Talmon Y, Minsky A, Ferrari ME, Wheeler CJ, Barenholz Y: Phase behavior, DNA ordering, and size instability of cationic lipoplexes. J Biol Chem. 2001, 276: 47453-47459. 10.1074/jbc.M105588200. (10.1074/jbc.M105588200) / J Biol Chem by D Simberg (2001)
  18. Lukyanov AN, Gao Z, Mazzola L, Torchilin VP: Polyethylene glycol-diacyllipid micelles demonstrate increased acculumation in subcutaneous tumors in mice. Pharm Res. 2002, 19: 1424-1429. 10.1023/A:1020488012264. (10.1023/A:1020488012264) / Pharm Res by AN Lukyanov (2002)
  19. Riboni L, Prinetti A, Bassi R, Caminiti A, Tettamant G: A mediator role of ceramide in the regulation of neuroblastoma neuro2a cell differentiation. J Biol Chem. 1995, 270: 26868-26875. 10.1074/jbc.270.45.26868. (10.1074/jbc.270.45.26868) / J Biol Chem by L Riboni (1995)
  20. Sato C, Matsuda T, Kitajima K: Neuronal differentiation-dependent expression of the disialic acid epitope on CD166 and its involvement in neurite formation in neuro2A cells. J Biol Chem. 2002, 277: 45299-45305. 10.1074/jbc.M206046200. (10.1074/jbc.M206046200) / J Biol Chem by C Sato (2002)
  21. Weissleder R, Pittet MJ: Imaging in the era of molecular oncology. Nature. 2008, 452: 580-589. 10.1038/nature06917. (10.1038/nature06917) / Nature by R Weissleder (2008)
  22. Dandamudi S, Campbell RB: Development and characterization of magnetic cationic liposomes for targeting tumor microvasculature. Biochim Biophys Acta. 2007, 1768: 427-438. 10.1016/j.bbamem.2006.10.006. (10.1016/j.bbamem.2006.10.006) / Biochim Biophys Acta by S Dandamudi (2007)
  23. Kawai N, Ito A, Nakahara Y, Futakuchi M, Shirai T, Honda H, Kobayashi T, Kohri K: Anticancer effect of hyperthermia on prostate cancer mediated by magnetite cationic liposomes and immune-response induction in transplanted syngeneic rats. Prostate. 2005, 64: 373-381. 10.1002/pros.20253. (10.1002/pros.20253) / Prostate by N Kawai (2005)
  24. Martina MS, Fortin JP, Menager C, Clement O, Barratt G, Grabielle-Madelmont C, Gazeau F, Cabuil V, Lesieur S: Generation of superparamagnetic liposomes revealed as highly efficient MRI contrast agents for in vivo imaging. J Am Chem Soc. 2005, 127: 10676-10685. 10.1021/ja0516460. (10.1021/ja0516460) / J Am Chem Soc by MS Martina (2005)
  25. Moghimi SM, Hunter AC, Murray JC: Long-circulating and target-specific nanoparticles: theory to practice. Pharmacol Rev. 2001, 53: 283-318. (10.1016/S0031-6997(24)01494-7) / Pharmacol Rev by SM Moghimi (2001)
  26. Immordino ML, Dosio F, Cattel L: Stealth liposomes: review of the basic science, rationale, and clinical applications, existing and potential. Int J Nanomedicine. 2006, 1: 297-315. 10.2217/17435889.1.3.297. (10.2217/17435889.1.3.297) / Int J Nanomedicine by ML Immordino (2006)
  27. Simberg D, Weisman S, Talmon Y, Barenholz Y: DOTAP (and other cationic lipids): chemistry, biophysics, and transfection. Crit Rev Ther Drug Carrier Syst. 2004, 21: 257-317. 10.1615/CritRevTherDrugCarrierSyst.v21.i4.10. (10.1615/CritRevTherDrugCarrierSyst.v21.i4.10) / Crit Rev Ther Drug Carrier Syst by D Simberg (2004)
  28. Lee CH, Ni YH, Chen CC, Chou C, Chang FH: Synergistic effect of polyethylenimine and cationic liposomes in nucleic acid delivery to human cancer cells. Biochim Biophys Acta. 2003, 1611: 55-62. 10.1016/S0005-2736(03)00027-0. (10.1016/S0005-2736(03)00027-0) / Biochim Biophys Acta by CH Lee (2003)
  29. Cinteza LO, Ohulchanskyy TY, Sahoo Y, Bergey EJ, Pandey RK, Prasad PN: Diacyllipid micelle-based nanocarrier for magnetically guided delivery of drugs in photodynamic therapy. Mol Pharm. 2006, 3: 415-23. 10.1021/mp060015p. (10.1021/mp060015p) / Mol Pharm by LO Cinteza (2006)
  30. Chang CF, Chen CY, Chang FH, Tai SP, Chen CY, Yu CH, Tseng YB, Tsai TH, Liu IS, Su WF, Sun CK: Cell tracking and detection of molecular expression in live cells using lipid-enclosed CdSe quantum dots as contrast agents for epi-third harmonic generation microscopy. Opt Express. 2008, 16: 9534-48. 10.1364/OE.16.009534. (10.1364/OE.16.009534) / Opt Express by CF Chang (2008)
  31. Krötz F, Sohn HY, Gloe T, Plank C, Pohl U: Magnetofection potentiates gene delivery to cultured endothelial cells. J Vasc Res. 2003, 40: 425-434. 10.1159/000073901. (10.1159/000073901) / J Vasc Res by F Krötz (2003)
  32. Pisanic TR, Blackwell JD, Shubayev VI, Fiñones RR, Jin S: Nanotoxicity of iron oxide nanoparticle internalization in growing neurons. Biomaterials. 2007, 28: 2572-2581. 10.1016/j.biomaterials.2007.01.043. (10.1016/j.biomaterials.2007.01.043) / Biomaterials by TR Pisanic II (2007)
  33. Kraitchman DL, Bulte JW: Imaging of stem cells using MRI. Basic Res Cardiol. 2008, 103: 105-113. 10.1007/s00395-008-0704-5. (10.1007/s00395-008-0704-5) / Basic Res Cardiol by DL Kraitchman (2008)
  34. Anderson SA, Shukaliak-Quandt J, Jordan EK, Arbab AS, Martin R, McFarland H, Frank JA: Magnetic resonance imaging of labeled T-cells in a mouse model of multiple sclerosis. Ann Neurol. 2004, 55: 654-659. 10.1002/ana.20066. (10.1002/ana.20066) / Ann Neurol by SA Anderson (2004)
  35. Kraitchman DL, Heldman AW, Atalar E, Amado LC, Martin BJ, Pittenger MF, Hare JM, Bulte JW: In vivo magnetic resonance imaging of mesenchymal stem cells in myocardial infarction. Circulation. 2003, 107: 2290-2293. 10.1161/01.CIR.0000070931.62772.4E. (10.1161/01.CIR.0000070931.62772.4E) / Circulation by DL Kraitchman (2003)
  36. Zhang Z, Jiang Q, Jiang F, Ding G, Zhang R, Wang L, Zhang L, Robin AM, Katakowski M, Chopp M: In vivo magnetic resonance imaging tracks adult neural progenitor cell targeting of brain tumor. Neuroimage. 2004, 23: 281-287. 10.1016/j.neuroimage.2004.05.019. (10.1016/j.neuroimage.2004.05.019) / Neuroimage by Z Zhang (2004)
  37. Guzman R, Uchida N, Bliss TM, He D, Christopherson KK, Stellwagen D, Capela A, Greve J, Malenka RC, Moseley ME, Palmer TD, Steinberg GK: Long-term monitoring of transplanted human neural stem cells in developmental and pathological contexts with MRI. Proc Natl Acad Sci USA. 2007, 104: 10211-10216. 10.1073/pnas.0608519104. (10.1073/pnas.0608519104) / Proc Natl Acad Sci USA by R Guzman (2007)
  38. Nelson GN, Roh JD, Mirensky TL, Wang Y, Yi T, Tellides G, Pober JS, Shkarin P, Shapiro EM, Saltzman WM, Papademetris X, Fahmy TM, Breuer CK: Initial evaluation of the use of USPIO cell labeling and noninvasive MR monitoring of human tissue-engineered vascular grafts in vivo. FASEB J. 2008, 22: 3888-95. 10.1096/fj.08-107367. (10.1096/fj.08-107367) / FASEB J by GN Nelson (2008)
Dates
Type When
Created 15 years, 11 months ago (Sept. 21, 2009, 8:27 a.m.)
Deposited 6 months, 2 weeks ago (Feb. 12, 2025, 3:14 a.m.)
Indexed 1 month, 3 weeks ago (July 2, 2025, 1:53 p.m.)
Issued 15 years, 11 months ago (Sept. 21, 2009)
Published 15 years, 11 months ago (Sept. 21, 2009)
Published Online 15 years, 11 months ago (Sept. 21, 2009)
Published Print 15 years, 8 months ago (Dec. 1, 2009)
Funders 0

None

@article{Huang_2009, title={Formulation of novel lipid-coated magnetic nanoparticles as the probe for in vivo imaging}, volume={16}, ISSN={1423-0127}, url={http://dx.doi.org/10.1186/1423-0127-16-86}, DOI={10.1186/1423-0127-16-86}, number={1}, journal={Journal of Biomedical Science}, publisher={Springer Science and Business Media LLC}, author={Huang, Huey-Chung and Chang, Po-Yuan and Chang, Karen and Chen, Chao-Yu and Lin, Chung-Wu and Chen, Jyh-Horng and Mou, Chung-Yuan and Chang, Zee-Fen and Chang, Fu-Hsiung}, year={2009}, month=sep }