DOI: 10.1182/blood.v99.6.2084. Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression

Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression

10.1182/blood.v99.6.2084

Crossref journal-article

American Society of Hematology

Blood (234)

Abstract

AbstractMouse spleen contains CD4+, CD8α+, and CD4−/CD8α− dendritic cells (DCs) in a 2:1:1 ratio. An analysis of 70 surface and cytoplasmic antigens revealed several differences in antigen expression between the 3 subsets. Notably, the Birbeck granule–associated Langerin antigen, as well as CD103 (the mouse homologue of the rat DC marker OX62), were specifically expressed by the CD8α+ DC subset. All DC types were apparent in the T-cell areas as well as in the splenic marginal zones and showed similar migratory capacity in collagen lattices. The 3 DC subtypes stimulated allogeneic CD4+ T cells comparably. However, CD8α+ DCs were very weak stimulators of resting or activated allogeneic CD8+ T cells, even at high stimulator-to-responder ratios, although this defect could be overcome under optimal DC/T cell ratios and peptide concentrations using CD8+ F5 T-cell receptor (TCR)–transgenic T cells. CD8α− or CD8α+DCs presented alloantigens with the same efficiency for lysis by cytotoxic T lymphocytes (CTLs), and their turnover rate of class I–peptide complexes was similar, thus neither an inability to present, nor rapid loss of antigenic complexes from CD8α DCs was responsible for the low allostimulatory capacity of CD8α+ DCs in vitro. Surprisingly, both CD8α+ DCs and CD4−/CD8− DCs efficiently primed minor histocompatibility (H-Y male antigen) cytotoxicity following intravenous injection, whereas CD4+ DCs were weak inducers of CTLs. Thus, the inability of CD8α+ DCs to stimulate CD8+ T cells is limited to certain in vitro assays that must lack certain enhancing signals present during in vivo interaction between CD8α+ DCs and CD8+ T cells.

Bibliography

McLellan, A. D., Kapp, M., Eggert, A., Linden, C., Bommhardt, U., BroÌcker, E.-B., KaÌmmerer, U., & KaÌmpgen, E. (2002). Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression. Blood, 99(6), 2084â2093.

Authors 8

Alexander D. McLellan (first)
Michaela Kapp (additional)
Andreas Eggert (additional)
Christian Linden (additional)
Ursula Bommhardt (additional)
Eva-B. Bröcker (additional)
Ulrike Kämmerer (additional)
Eckhart Kämpgen (additional)

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Dates

Type	When
Created	22 years, 10 months ago (Oct. 1, 2002, 5:48 p.m.)
Deposited	2 years, 4 months ago (April 24, 2023, 3:37 p.m.)
Indexed	2 weeks ago (Aug. 12, 2025, 6:04 p.m.)
Issued	23 years, 5 months ago (March 15, 2002)
Published	23 years, 5 months ago (March 15, 2002)
Published Print	23 years, 5 months ago (March 15, 2002)

Funders 0

None

BibTeX

@article{McLellan_2002, title={Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression}, volume={99}, ISSN={0006-4971}, url={http://dx.doi.org/10.1182/blood.v99.6.2084}, DOI={10.1182/blood.v99.6.2084}, number={6}, journal={Blood}, publisher={American Society of Hematology}, author={McLellan, Alexander D. and Kapp, Michaela and Eggert, Andreas and Linden, Christian and Bommhardt, Ursula and Bröcker, Eva-B. and Kämmerer, Ulrike and Kämpgen, Eckhart}, year={2002}, month=mar, pages={2084–2093} }

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