DOI: 10.1182/blood.v97.6.1854. Platelet-endothelial cell adhesion molecule-1 (PECAM-1)–deficient mice demonstrate a transient and cytokine-specific role for PECAM-1 in leukocyte migration through the perivascular basement membrane

Platelet-endothelial cell adhesion molecule-1 (PECAM-1)–deficient mice demonstrate a transient and cytokine-specific role for PECAM-1 in leukocyte migration through the perivascular basement membrane

10.1182/blood.v97.6.1854

Crossref journal-article

American Society of Hematology

Blood (234)

Abstract

Studies with neutralizing antibodies have indicated roles for platelet-endothelial cell adhesion molecule-1 (PECAM-1) in leukocyte migration through the endothelium and the perivascular basement membrane. Because some of these findings have been contentious, this study aimed to explore the role of PECAM-1 in leukocyte migration by analyzing leukocyte responses in interleukin 1β (IL-1β)– and tumor necrosis factor-α (TNFα)–activated cremasteric venules of PECAM-1–deficient mice using intravital and electron microscopy. Although no differences in levels of leukocyte rolling flux or firm adhesion were observed, a delay in leukocyte transmigration in response to IL-1β, but not TNFα, was detected in PECAM-1–deficient mice. Electron microscopy indicated that this delay occurred at the level of perivascular basement membrane. To address the cytokine specificity of PECAM-1 dependence, in vitro experiments demonstrated that TNFα, but not IL-1β, could induce rapid adhesion of murine neutrophils to protein-coated surfaces, suggesting that TNFα elicited leukocyte transmigration in wild-type mice via direct stimulation of leukocytes. In summary, the results suggest a regulatory role for PECAM-1 in leukocyte migration through the perivascular basement membrane, a role that appears to be cytokine-specific and associated with the ability of the cytokine to stimulate rapid neutrophil adhesion.

Bibliography

Thompson, R. D., Noble, K. E., Larbi, K. Y., Dewar, A., Duncan, G. S., Mak, T. W., & Nourshargh, S. (2001). Platelet-endothelial cell adhesion molecule-1 (PECAM-1)âdeficient mice demonstrate a transient and cytokine-specific role for PECAM-1 in leukocyte migration through the perivascular basement membrane. Blood, 97(6), 1854â1860.

Authors 7

Richard D. Thompson (first)
Karen E. Noble (additional)
Karen Y. Larbi (additional)
Ann Dewar (additional)
Gordon S. Duncan (additional)
Tak W. Mak (additional)
Sussan Nourshargh (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 8:46 a.m.)
Deposited	1 year, 7 months ago (Jan. 6, 2024, 1:43 a.m.)
Indexed	10 hours, 6 minutes ago (Sept. 3, 2025, 6:04 a.m.)
Issued	24 years, 5 months ago (March 15, 2001)
Published	24 years, 5 months ago (March 15, 2001)
Published Print	24 years, 5 months ago (March 15, 2001)

Funders 0

None

BibTeX

@article{Thompson_2001, title={Platelet-endothelial cell adhesion molecule-1 (PECAM-1)–deficient mice demonstrate a transient and cytokine-specific role for PECAM-1 in leukocyte migration through the perivascular basement membrane}, volume={97}, ISSN={0006-4971}, url={http://dx.doi.org/10.1182/blood.v97.6.1854}, DOI={10.1182/blood.v97.6.1854}, number={6}, journal={Blood}, publisher={American Society of Hematology}, author={Thompson, Richard D. and Noble, Karen E. and Larbi, Karen Y. and Dewar, Ann and Duncan, Gordon S. and Mak, Tak W. and Nourshargh, Sussan}, year={2001}, month=mar, pages={1854–1860} }

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