DOI: 10.1182/blood.v97.4.901. mDYRK3 kinase is expressed selectively in late erythroid progenitor cells and attenuates colony-forming unit

mDYRK3 kinase is expressed selectively in late erythroid progenitor cells and attenuates colony-forming unit–erythroid development

10.1182/blood.v97.4.901

Crossref journal-article

American Society of Hematology

Blood (234)

Abstract

AbstractDYRKs are a new subfamily of dual-specificity kinases that was originally discovered on the basis of homology to Yak1, an inhibitor of cell cycle progression in yeast. At present, mDYRK-3 and mDYRK-2 have been cloned, and mDYRK-3 has been characterized with respect to kinase activity, expression among tissues and hematopoietic cells, and possible function during erythropoiesis. In sequence, mDYRK-3 diverges markedly in noncatalytic domains from mDYRK-2 and mDYRK-1a, but is 91.3% identical overall to hDYRK-3. Catalytically, mDYRK-3 readily phosphorylated myelin basic protein (but not histone 2B) and also appeared to autophosphorylate in vitro. Expression of mDYRK-1a, mDYRK-2, and mDYRK-3 was high in testes, but unlike mDYRK1a and mDYRK 2, mDYRK-3 was not expressed at appreciable levels in other tissues examined. Among hematopoietic cells, however, mDYRK-3 expression was selectively elevated in erythroid cell lines and primary pro-erythroid cells. In developmentally synchronized erythroid progenitor cells, expression peaked sharply following exposure to erythropoietin plus stem cell factor (SCF) (but not SCF alone), and in situ hybridizations of sectioned embryos revealed selective expression of mDYRK-3 in fetal liver. Interestingly, antisense oligonucleotides to mDYRK-3 were shown to significantly and specifically enhance colony-forming unit–erythroid colony formation. Thus, it is proposed that mDYRK-3 kinase functions as a lineage-restricted, stage-specific suppressor of red cell development.

Bibliography

Geiger, J. N., Knudsen, G. T., Panek, L., Pandit, A. K., Yoder, M. D., Lord, K. A., Creasy, C. L., Burns, B. M., Gaines, P., Dillon, S. B., & Wojchowski, D. M. (2001). mDYRK3 kinase is expressed selectively in late erythroid progenitor cells and attenuates colony-forming unitâerythroid development. Blood, 97(4), 901â910.

Authors 11

Justin N. Geiger (first)
Geoffry T. Knudsen (additional)
Leigh Panek (additional)
Ajay K. Pandit (additional)
Michael D. Yoder (additional)
Kenneth A. Lord (additional)
Caretha L. Creasy (additional)
Brian M. Burns (additional)
Peter Gaines (additional)
Susan B. Dillon (additional)
Don M. Wojchowski (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 8:46 a.m.)
Deposited	4 years, 3 months ago (May 26, 2021, 5:15 a.m.)
Indexed	2 months, 1 week ago (June 18, 2025, 1:46 a.m.)
Issued	24 years, 6 months ago (Feb. 15, 2001)
Published	24 years, 6 months ago (Feb. 15, 2001)
Published Print	24 years, 6 months ago (Feb. 15, 2001)

Funders 0

None

BibTeX

@article{Geiger_2001, title={mDYRK3 kinase is expressed selectively in late erythroid progenitor cells and attenuates colony-forming unit–erythroid development}, volume={97}, ISSN={0006-4971}, url={http://dx.doi.org/10.1182/blood.v97.4.901}, DOI={10.1182/blood.v97.4.901}, number={4}, journal={Blood}, publisher={American Society of Hematology}, author={Geiger, Justin N. and Knudsen, Geoffry T. and Panek, Leigh and Pandit, Ajay K. and Yoder, Michael D. and Lord, Kenneth A. and Creasy, Caretha L. and Burns, Brian M. and Gaines, Peter and Dillon, Susan B. and Wojchowski, Don M.}, year={2001}, month=feb, pages={901–910} }

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