DOI: 10.1182/blood.v97.4.1141. Stomatin, flotillin-1, and flotillin-2 are major integral proteins of erythrocyte lipid rafts

Stomatin, flotillin-1, and flotillin-2 are major integral proteins of erythrocyte lipid rafts

10.1182/blood.v97.4.1141

Crossref journal-article

American Society of Hematology

Blood (234)

Abstract

AbstractLipid rafts are sphingolipid- and cholesterol-rich membrane microdomains that are insoluble in nonionic detergents, have a low buoyant density, and preferentially contain lipid-modified proteins, like glycosyl phosphatidylinositol (GPI)-anchored proteins. The lipid rafts were isolated from human erythrocytes and major protein components were identified. Apart from the GPI-anchored proteins, the most abundant integral proteins were found to be the distantly related membrane proteins stomatin (band 7.2b), flotillin-1, and flotillin-2. Flotillins, already described as lipid raft components in neurons and caveolae-associated proteins in A498 kidney cells, have not been recognized as red cell components yet. In addition, it was shown that the major cytoskeletal proteins, spectrin, actin, band 4.1, and band 4.2, are partly associated with the lipid rafts. Stomatin and the flotillins are present as independently organized high-order oligomers, suggesting that these complexes act as separate scaffolding components at the cytoplasmic face of erythrocyte lipid rafts.

Bibliography

Salzer, U., & Prohaska, R. (2001). Stomatin, flotillin-1, and flotillin-2 are major integral proteins of erythrocyte lipid rafts. Blood, 97(4), 1141â1143.

Authors 2

Ulrich Salzer (first)
Rainer Prohaska (additional)

References 25 Referenced 283

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10.1074/jbc.273.27.17221 / J Biol Chem. / Oligomeric nature of the integral membrane protein stomatin. by Snyers (1998)
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10.1182/blood.V93.7.2404 / Blood. / Stomatocytosis is absent in “stomatin”-deficient murine red blood cells. by Zhu (1999)
10.1016/S0968-0004(99)01467-X / Trends Biochem Sci. / The SPFH domain: implicated in regulating targeted protein turnover in stomatins and other membrane-associated proteins. by Tavernarakis (1999)

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 8:46 a.m.)
Deposited	4 years, 3 months ago (May 26, 2021, 5:16 a.m.)
Indexed	1 day, 18 hours ago (Sept. 4, 2025, 9:12 a.m.)
Issued	24 years, 6 months ago (Feb. 15, 2001)
Published	24 years, 6 months ago (Feb. 15, 2001)
Published Print	24 years, 6 months ago (Feb. 15, 2001)

Funders 0

None

BibTeX

@article{Salzer_2001, title={Stomatin, flotillin-1, and flotillin-2 are major integral proteins of erythrocyte lipid rafts}, volume={97}, ISSN={0006-4971}, url={http://dx.doi.org/10.1182/blood.v97.4.1141}, DOI={10.1182/blood.v97.4.1141}, number={4}, journal={Blood}, publisher={American Society of Hematology}, author={Salzer, Ulrich and Prohaska, Rainer}, year={2001}, month=feb, pages={1141–1143} }

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