DOI: 10.1182/blood.v96.9.3086. The human antimicrobial and chemotactic peptides LL-37 and α-defensins are expressed by specific lymphocyte and monocyte populations

The human antimicrobial and chemotactic peptides LL-37 and α-defensins are expressed by specific lymphocyte and monocyte populations

10.1182/blood.v96.9.3086

Crossref journal-article

American Society of Hematology

Blood (234)

Abstract

AbstractWe identified antibacterial components in human T and natural killer (NK) cells by using freshly isolated lymphocytes enriched for T and NK cells as starting material. After growing these lymphocytes for 5 days in the presence of interleukin (IL)–2, we isolated and characterized several antibacterial peptides/proteins from the supernatant—α-defensins (HNP 1-3), LL-37, lysozyme, and a fragment of histone H2B—although other active components were also present. We then used reverse transcriptase–polymerase chain reaction to search for expression of the gene coding for LL-37 in several B-cell lines, γδ T-cell lines, NK clones, and one monocytic cell line, with positive results, but found no expression in several αβ T-cell lines. The α-defensins (HNP 1-3) were also found to be expressed in several of these cell lines. To confirm the presence of these antibacterial peptides in lymphocytes, we localized them to NK, γδ T cells, B cells, and monocytes/macrophages by using double-staining immunohistochemical analysis of freshly isolated lymphocytes. We also found that primary cultures of lymphocytes transcribe and secrete LL-37 and that these processes are affected by IL-6 and interferon-γ. In addition, we demonstrated that LL-37 has chemotactic activity for polymorphonuclear leukocytes and CD4 T lymphocytes, whereas others have shown chemotactic activity for human α-defensins (HNP 1-2). These findings suggest that microbicidal peptides are effector molecules of lymphocytes and that antibacterial activity previously shown to be derived from T and NK cells may be partly mediated by the antibacterial peptides LL-37 and HNP 1-3.

Bibliography

Agerberth, B., Charo, J., Werr, J., Olsson, B., Idali, F., Lindbom, L., Kiessling, R., JoÌrnvall, H., Wigzell, H., & Gudmundsson, G. H. (2000). The human antimicrobial and chemotactic peptides LL-37 and Î±-defensins are expressed by specific lymphocyte and monocyte populations. Blood, 96(9), 3086â3093.

Authors 10

Birgitta Agerberth (first)
Jehad Charo (additional)
Joachim Werr (additional)
Berit Olsson (additional)
Farah Idali (additional)
Lennart Lindbom (additional)
Rolf Kiessling (additional)
Hans Jörnvall (additional)
Hans Wigzell (additional)
Gudmundur H. Gudmundsson (additional)

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Dates

Type	When
Created	5 years, 10 months ago (Oct. 14, 2019, 12:44 a.m.)
Deposited	1 year, 11 months ago (Sept. 21, 2023, 12:06 p.m.)
Indexed	5 days, 17 hours ago (Aug. 31, 2025, 6:32 a.m.)
Issued	24 years, 10 months ago (Nov. 1, 2000)
Published	24 years, 10 months ago (Nov. 1, 2000)
Published Print	24 years, 10 months ago (Nov. 1, 2000)

Funders 0

None

BibTeX

@article{Agerberth_2000, title={The human antimicrobial and chemotactic peptides LL-37 and α-defensins are expressed by specific lymphocyte and monocyte populations}, volume={96}, ISSN={0006-4971}, url={http://dx.doi.org/10.1182/blood.v96.9.3086}, DOI={10.1182/blood.v96.9.3086}, number={9}, journal={Blood}, publisher={American Society of Hematology}, author={Agerberth, Birgitta and Charo, Jehad and Werr, Joachim and Olsson, Berit and Idali, Farah and Lindbom, Lennart and Kiessling, Rolf and Jörnvall, Hans and Wigzell, Hans and Gudmundsson, Gudmundur H.}, year={2000}, month=nov, pages={3086–3093} }

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