Concept of lymphoid versus myeloid dendritic cell lineages revisited: both CD8α− and CD8α+dendritic cells are generated from CD4lowlymphoid-committed precursors
10.1182/blood.v96.7.2511
Crossref journal-article
American Society of Hematology
Blood (234)
Abstract

AbstractTwo dendritic cell (DC) subsets have been identified in the murine system on the basis of their differential CD8α expression. CD8α+ DCs and CD8α− DCs are considered as lymphoid- and myeloid-derived, respectively, because CD8α+ but not CD8α− splenic DCs were generated from lymphoid CD4low precursors, devoid of myeloid reconstitution potential. Although CD8α− DCs were first described as negative for CD4, our results demonstrate that approximately 70% of them are CD4+. Besides CD4− CD8α− and CD4+CD8α− DCs displayed a similar phenotype and T-cell stimulatory potential in mixed lymphocyte reaction (MLR), although among CD8α− DCs, the CD4+ subset appears to have a higher endocytic capacity. Finally, experiments of DC reconstitution after irradiation in which, in contrast to previous studies, donor-type DCs were analyzed without depleting CD4+ cells, revealed that both CD8α+ DCs and CD8α− DCs were generated after transfer of CD4low precursors. These data suggest that both CD8α+ and CD8α− DCs derive from a common precursor and, hence, do not support the concept of the CD8α+ lymphoid-derived and CD8α−myeloid-derived DC lineages. However, because this hypothesis has to be confirmed at the clonal level, it remains possible that CD8α− DCs arise from a myeloid precursor within the CD4low precursor population or, alternatively, that both CD8α+ and CD8α− DCs derive from an independent nonlymphoid, nonmyeloid DC precursor. In conclusion, although we favor the hypothesis that both CD8α+ and CD8α− DCs derive from a lymphoid-committed precursor, a precise study of the differentiation process of CD8α+ and CD8α− DCs is required to define conclusively their origin.

Bibliography

Martı́n, P., del Hoyo, G. M., Anjuère, F., Ruiz, S. R., Arias, C. F., Marı́n, A. R., & Ardavı́n, C. (2000). Concept of lymphoid versus myeloid dendritic cell lineages revisited: both CD8α− and CD8α+dendritic cells are generated from CD4lowlymphoid-committed precursors. Blood, 96(7), 2511–2519.

Authors 7
  1. Pilar Martı́n (first)
  2. Gloria Martı́nez del Hoyo (additional)
  3. Fabienne Anjuère (additional)
  4. Sara Ruiz Ruiz (additional)
  5. Cristina Fernández Arias (additional)
  6. Alvaro Rodrı́guez Marı́n (additional)
  7. Carlos Ardavı́n (additional)
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Dates
Type When
Created 5 years, 10 months ago (Oct. 14, 2019, 1:31 a.m.)
Deposited 1 year, 11 months ago (Sept. 21, 2023, 12:08 p.m.)
Indexed 5 months ago (March 30, 2025, 1:25 a.m.)
Issued 24 years, 11 months ago (Oct. 1, 2000)
Published 24 years, 11 months ago (Oct. 1, 2000)
Published Print 24 years, 11 months ago (Oct. 1, 2000)
Funders 0

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@article{Mart_n_2000, title={Concept of lymphoid versus myeloid dendritic cell lineages revisited: both CD8α− and CD8α+dendritic cells are generated from CD4lowlymphoid-committed precursors}, volume={96}, ISSN={0006-4971}, url={http://dx.doi.org/10.1182/blood.v96.7.2511}, DOI={10.1182/blood.v96.7.2511}, number={7}, journal={Blood}, publisher={American Society of Hematology}, author={Martı́n, Pilar and del Hoyo, Gloria Martı́nez and Anjuère, Fabienne and Ruiz, Sara Ruiz and Arias, Cristina Fernández and Marı́n, Alvaro Rodrı́guez and Ardavı́n, Carlos}, year={2000}, month=oct, pages={2511–2519} }