DOI: 10.1182/blood.v96.5.1865. Differentiation of myeloid dendritic cells into CD8α-positive dendritic cells in vivo

Differentiation of myeloid dendritic cells into CD8α-positive dendritic cells in vivo

10.1182/blood.v96.5.1865

Crossref journal-article

American Society of Hematology

Blood (234)

Abstract

AbstractBone marrow-derived dendritic cells (DC) represent a family of antigen-presenting cells (APC) with varying phenotypes. For example, in mice, CD8α+ and CD8α− DC are thought to represent cells of lymphoid and myeloid origin, respectively. Langerhans cells (LC) of the epidermis are typical myeloid DC; they do not express CD8α, but they do express high levels of myeloid antigens such as CD11b and FcγR. By contrast, thymic DC, which derive from a lymphoid-related progenitor, express CD8α but only low levels of myeloid antigens. CD8α+ DC are also found in the spleen and lymph nodes (LN), but the origin of these cells has not been determined. By activating and labeling CD8α− epidermal LC in vivo, it was found that these cells expressed CD8α on migration to the draining LN. Similarly, CD8α− LC generated in vitro from a CD8 wild-type mouse and injected into the skin of a CD8αKO mouse expressed CD8α when they reached the draining LN. The results also show that CD8α+ LC are potent APC. After migration from skin, they localized in the T-cell areas of LN, secreted high levels of interleukin-12, interferon-γ, and chemokine-attracting T cells, and they induced antigen-specific T-cell activation. These results demonstrate that myeloid DC in the periphery can express CD8α when they migrate to the draining LN. CD8α expression on these DC appears to reflect a state of activation, mobilization, or both, rather than lineage.

Bibliography

Merad, M., Fong, L., Bogenberger, J., & Engleman, E. G. (2000). Differentiation of myeloid dendritic cells into CD8Î±-positive dendritic cells in vivo. Blood, 96(5), 1865â1872.

Authors 4

Miriam Merad (first)
Lawrence Fong (additional)
Jakob Bogenberger (additional)
Edgar G. Engleman (additional)

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Dates

Type	When
Created	5 years, 10 months ago (Oct. 14, 2019, 5:37 a.m.)
Deposited	1 year, 11 months ago (Sept. 21, 2023, 12:43 p.m.)
Indexed	4 months, 4 weeks ago (March 30, 2025, 11:26 a.m.)
Issued	24 years, 11 months ago (Sept. 1, 2000)
Published	24 years, 11 months ago (Sept. 1, 2000)
Published Print	24 years, 11 months ago (Sept. 1, 2000)

Funders 0

None

BibTeX

@article{Merad_2000, title={Differentiation of myeloid dendritic cells into CD8α-positive dendritic cells in vivo}, volume={96}, ISSN={0006-4971}, url={http://dx.doi.org/10.1182/blood.v96.5.1865}, DOI={10.1182/blood.v96.5.1865}, number={5}, journal={Blood}, publisher={American Society of Hematology}, author={Merad, Miriam and Fong, Lawrence and Bogenberger, Jakob and Engleman, Edgar G.}, year={2000}, month=sep, pages={1865–1872} }

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