DOI: 10.1182/blood.v95.8.2543. GATA-1 interacts with the myeloid PU.1 transcription factor and represses PU.1-dependent transcription

GATA-1 interacts with the myeloid PU.1 transcription factor and represses PU.1-dependent transcription

10.1182/blood.v95.8.2543

Crossref journal-article

American Society of Hematology

Blood (234)

Abstract

AbstractThe GATA-1 transcription factor is capable of suppressing the myeloid gene expression program when ectopically expressed in myeloid cells. We examined the ability of GATA-1 to repress the expression and function of the PU.1 transcription factor, a central regulator of myeloid differentiation. We found that GATA-1 is capable of suppressing the myeloid phenotype without interfering with PU.1 gene expression, but instead was capable of inhibiting the activity of the PU.1 protein in a dose-dependent manner. This inhibition was independent of the ability of GATA-1 to bind DNA, suggesting that it is mediated by protein-protein interaction. We examined the ability of PU.1 to interact with GATA-1 and found a direct interaction between the PU.1 ETS domain and the C-terminal finger region of GATA-1. Replacing the PU.1 ETS domain with the GAL4 DNA-binding domain removed the ability of GATA-1 to inhibit PU.1 activity, indicating that the PU.1 DNA-binding domain, rather than the transactivation domain, is the target for GATA-1–mediated repression. We therefore propose that GATA-1 represses myeloid gene expression, at least in part, through its ability to directly interact with the PU.1 ETS domain and thereby interfere with PU.1 function.

Bibliography

Nerlov, C., Querfurth, E., Kulessa, H., & Graf, T. (2000). GATA-1 interacts with the myeloid PU.1 transcription factor and represses PU.1-dependent transcription. Blood, 95(8), 2543â2551.

Authors 4

Claus Nerlov (first)
Erich Querfurth (additional)
Holger Kulessa (additional)
Thomas Graf (additional)

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Dates

Type	When
Created	5 years, 10 months ago (Oct. 14, 2019, 12:04 a.m.)
Deposited	1 year, 11 months ago (Sept. 21, 2023, 12:06 p.m.)
Indexed	2 weeks, 1 day ago (Aug. 21, 2025, 12:51 p.m.)
Issued	25 years, 4 months ago (April 15, 2000)
Published	25 years, 4 months ago (April 15, 2000)
Published Print	25 years, 4 months ago (April 15, 2000)

Funders 0

None

BibTeX

@article{Nerlov_2000, title={GATA-1 interacts with the myeloid PU.1 transcription factor and represses PU.1-dependent transcription}, volume={95}, ISSN={0006-4971}, url={http://dx.doi.org/10.1182/blood.v95.8.2543}, DOI={10.1182/blood.v95.8.2543}, number={8}, journal={Blood}, publisher={American Society of Hematology}, author={Nerlov, Claus and Querfurth, Erich and Kulessa, Holger and Graf, Thomas}, year={2000}, month=apr, pages={2543–2551} }

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