DOI: 10.1182/blood.v93.9.2771. Mice Homozygous for a Truncated Form of CREB-Binding Protein Exhibit Defects in Hematopoiesis and Vasculo-angiogenesis

Mice Homozygous for a Truncated Form of CREB-Binding Protein Exhibit Defects in Hematopoiesis and Vasculo-angiogenesis

10.1182/blood.v93.9.2771

Crossref journal-article

American Society of Hematology

Blood (234)

Abstract

Abstract CREB-binding protein (CBP) and the closely related adenovirus E1A-associated 300-kD protein (p300) function as coactivators of transcription factors such as CREB, c-Fos, c-Jun, c-Myb, and several nuclear receptors. To study the roles of CBP in embryonic development, we generated CBP homozygous mutant mouse embryos that expressed a truncated form of CBP protein (1-1084 out of 2441 residues). The embryos died between embryonic days 9.5 (E9.5) and E10.5 and exhibited a defect in neural tube closure. They appeared pale and showed decreases in erythroid cells and colony-forming cells (CFCs) in the yolk sac, suggesting defects in primitive hematopoiesis. Immunohistochemistry with an anti-PECAM antibody showed a lack of vascular network formation. Organ culture of para-aortic splanchnopleural mesoderm (P-Sp) with stromal cells (OP9) showed an autonomous abnormality of putative endothelial precursors, which may induce the microenvironmental defect in hematopoiesis. In addition, these defects were partially rescued by the addition of VEGF to this culture. Our analyses demonstrate that CBP plays an essential role in hematopoiesis and vasculo-angiogenesis.

Bibliography

Oike, Y., Takakura, N., Hata, A., Kaname, T., Akizuki, M., Yamaguchi, Y., Yasue, H., Araki, K., Yamamura, K., & Suda, T. (1999). Mice Homozygous for a Truncated Form of CREB-Binding Protein Exhibit Defects in Hematopoiesis and Vasculo-angiogenesis. Blood, 93(9), 2771â2779.

Authors 10

Yuichi Oike (first)
Nobuyuki Takakura (additional)
Akira Hata (additional)
Tadashi Kaname (additional)
Miwa Akizuki (additional)
Yuji Yamaguchi (additional)
Hirofumi Yasue (additional)
Kimi Araki (additional)
Ken-ichi Yamamura (additional)
Toshio Suda (additional)

References 31 Referenced 144

10.1038/365855a0 / Nature / Phosphorylated CREB binds specifically to the nuclear protein CBP. by Chrivia (1993)
10.1038/370223a0 / Nature / Nuclear protein CBP is a coactivator for the transcription factor CREB. by Kwok (1994)
10.1002/j.1460-2075.1995.tb00157.x / EMBO J / CBP-induced stimulation of c-Fos activity is abrogated by E1a. by Bannister (1995)
10.1038/370226a0 / Nature / Activation of cAMP and mitogen responsive genes relies on a common nuclear factor. by Arias (1994)
10.1101/gad.10.5.528 / Genes Dev / CBP as a transcriptional coactivator of c-Myb. by Dai (1996)
10.1016/S0092-8674(00)81118-6 / Cell / A CBP integrator complex mediates transcriptional activation and AP-1 inhibition by nuclear receptors. by Kamei (1996)
10.1038/383099a0 / Nature / Role of CBP/P300 in nuclear receptor signalling. by Chakravarti (1996)
10.1016/S0168-9525(98)01438-3 / Trends Genet / Conjunction dysfunction: CBP/p300 in human disease. by Giles (1998)
10.1016/S0962-8924(97)01048-9 / Trends Cell Biol / The p300/CBP family: Integrating signals with transcription factors and chromatin. by Shikama (1997)
10.1038/376348a0 / Nature / Rubinstein-Taybi syndrome caused by mutations in the transcriptional co-activator CBP. by Petrij (1995)
10.1093/hmg/8.3.387 / Hum Mol Genet / Truncated CBP protein leads to classical Rubinstein-Taybi syndrome phenotypes in mice: Implication for a dominant negative mechanism. by Oike (1999)
10.1073/pnas.94.19.10215 / Proc Natl Acad Sci USA / Abnormal skeletal patterning in embryos lacking a single Cbp allele: A partial similarity with Rubinstein-Taybi syndrome. by Tanaka (1997)
10.1016/S0092-8674(00)81165-4 / Cell / Gene dosage-dependent embryonic development and proliferation defects in mice lacking the transcriptional integrator p300. by Yao (1998)
10.1038/258726a0 / Nature / Role of stem cell migration in initiation of mouse foetal liver hematopoiesis. by Johnson (1975)
10.1038/364064a0 / Nature / An early pre-liver intraembryonic source of CFU-S in the developing mouse. by Medvinsky (1993)
10.1016/1074-7613(94)90081-7 / Immunity / Development of hematopoietic stem cell activity in the mouse embryo. by Müller (1994)
10.1016/S0092-8674(00)80165-8 / Cell / Definitive hematopoiesis is autonomously initiated by the AGM region. by Medvinsky (1996)
10.1016/S0092-8674(00)80166-X / Cell / Lymphoid potential, probed before circulation in mouse, is restricted to caudal intraembryonic splanchnopleura. by Cumano (1996)
10.1016/0092-8674(86)90519-2 / Cell / Tissue-specific in vitro transcription from the mouse albumin promoter. by Gorski (1986)
10.1038/227680a0 / Nature / Cleavage of structural proteins during the assembly of the bacteriophage T4. by Laemmli (1970)
10.1038/376348a0 / Nature / Rubinstein-Taybi syndrome caused by mutations in the transcriptional co-activator CBP. by Petrij (1995)
10.1016/S1074-7613(00)80665-2 / Immunity / Critical role of the TIE2 endotherial cell receptor in the development of definitive hematopoiesis. by Takakura (1998)
10.1084/jem.184.6.2301 / J Exp Med / Preferential proliferation of murine colony-forming units in culture in a chemically defined condition with a macrophage colony-stimulating factor-negative stromal cell clone. by Takakura (1996)
10.1126/science.2497519 / Science / Mouse embryonic stem cells and reporter constructs to detect developmentally regulated genes. by Gossler (1989)
10.1101/gad.6.6.903 / Genes Dev / A gene trap appoach in mouse embryoic stem cells: The lacZ reporter is activated by splicing, reflects endogenous gene expression, and is mutagenic in mice. by Skarnes (1998)
10.1002/jcp.1040830218 / J Cell Physio / Erythroid colony formation in cultures of mouse and human bone marrow analysis chromatography on agarose-concanavalin A. by Iscove (1974)
10.1177/002215549704500613 / J Histochem Cytochem / PDGFR alpha expression during mouse embryogenesis: Immuno-localization analyzed by whole-mount immunohistostaining using the monoclonal anti-mouse PDGFR alpha antibody APA5. by Takakura (1997)
10.1126/science.8066449 / Science / Generation of lympho-hematopoietic cells from embryonic stem cells in culture. by Nakano (1994)
10.1073/pnas.95.5.2061 / Proc Natl Acad Sci USA / CREB-binding protein cooperates with transcription factor GATA-1 and is required for erythroid differentiation. by Blobel (1998)
10.1128/MCB.18.4.2218 / Mol Cell Biol / A role for CREB binding protein and p300 transcriptional coactivators in Ets-1 transactivation functions. by Yang (1998)
10.1073/pnas.95.16.9506 / Proc Natl Acad Sci USA / CREB binding protein is a required coactivator for Smad-dependent, transforming growth factor β transcriptional responses in endothelial cells. by Topper (1998)

Dates

Type	When
Created	5 years, 10 months ago (Oct. 9, 2019, 10:40 p.m.)
Deposited	5 years, 6 months ago (Feb. 12, 2020, 5:12 p.m.)
Indexed	1 day, 22 hours ago (Sept. 2, 2025, 6:53 a.m.)
Issued	26 years, 4 months ago (May 1, 1999)
Published	26 years, 4 months ago (May 1, 1999)
Published Print	26 years, 4 months ago (May 1, 1999)

Funders 0

None

BibTeX

@article{Oike_1999, title={Mice Homozygous for a Truncated Form of CREB-Binding Protein Exhibit Defects in Hematopoiesis and Vasculo-angiogenesis}, volume={93}, ISSN={0006-4971}, url={http://dx.doi.org/10.1182/blood.v93.9.2771}, DOI={10.1182/blood.v93.9.2771}, number={9}, journal={Blood}, publisher={American Society of Hematology}, author={Oike, Yuichi and Takakura, Nobuyuki and Hata, Akira and Kaname, Tadashi and Akizuki, Miwa and Yamaguchi, Yuji and Yasue, Hirofumi and Araki, Kimi and Yamamura, Ken-ichi and Suda, Toshio}, year={1999}, month=may, pages={2771–2779} }

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