DOI: 10.1182/blood.v93.6.1916.406k15_1916_1921. Hematopoietic Stem Cell Tracking In Vivo: A Comparison of Short-Term and Long-Term Repopulating Cells

Hematopoietic Stem Cell Tracking In Vivo: A Comparison of Short-Term and Long-Term Repopulating Cells

10.1182/blood.v93.6.1916.406k15_1916_1921

Crossref journal-article

American Society of Hematology

Blood (234)

Abstract

We have previously demonstrated that we could separate long-term repopulating stem cells from cells that provided radioprotection (short-term repopulating cells) on the basis of size and suggested that this might be due to the quiescent nature of long-term repopulating cells. To further define the activity of these populations, we used a dye (PKH26), which incorporates into the membrane of cells and is equally distributed to daughter cells when they divide. We developed an assay, which allowed us to retrieve PKH26+ long-term and short-term repopulating cells in the hematopoietic tissues of the recipients posttransplant. We were able to recover the labeled cells and determine their cell cycle activity, as well as their ability to reconstitute secondary lethally irradiated hosts in limiting dilution. The results of our assay suggest that long-term repopulating cells are quiescent in the bone marrow (BM) 48 hours after transplant. We were able to detect only a few labeled cells in the peripheral blood posttransplant and even though cells homed to both the spleen and BM, more long-term repopulating cells homed to the marrow and only these cells, which homed to the marrow, were capable of reconstituting lethally irradiated secondary hosts long-term.

Bibliography

Lanzkron, S. M., Collector, M. I., & Sharkis, S. J. (1999). Hematopoietic Stem Cell Tracking In Vivo: A Comparison of Short-Term and Long-Term Repopulating Cells. Blood, 93(6), 1916â1921.

Authors 3

Sophie M. Lanzkron (first)
Michael I. Collector (additional)
Saul J. Sharkis (additional)

References 18 Referenced 131

10.1038/347188a0 / Nature / Separation of pluripotent haematopoietic stem cells from spleen colony-forming cells. by Jones (1990)
10.1182/blood.V88.2.487.bloodjournal882487 / Blood / Characterization of mouse lymphohematopoietic stem cells lacking spleen colony-forming activity. by Jones (1996)
10.1073/pnas.84.13.4485 / Proc Natl Acad Sci USA / In vitro homing of hemopoietic stem cells is mediated by a recognition system with galactosyl and mannosyl specificities. by Aizawa (1987)
10.1016/S0021-9258(19)47046-4 / J Biol Chem / Purification and partial characterization of membrane-homing receptors in two cloned murine hemopoietic progenitor cell lines. by Matsuoka (1989)
10.1073/pnas.92.21.9647 / Proc Natl Acad Sci USA / The VLA4/VCAM-1 adhesion pathway defines contrasting mechanisms of lodgement of transplanted murine hemopoietic progenitors between bone marrow and spleen. by Papayannopoulou (1995)
10.1182/blood.V85.9.2598.bloodjournal8592598 / Blood / Marrow and spleen-seeding efficiencies of all murine hematopoietic stem cell subsets are decreased by preincubation with hematopoietic growth factors. by van der Loo (1995)
{'key': '2020021216091867400_B7', 'first-page': '129', 'article-title': 'Homing of fluorescently labeled murine hematopoietic stem cells.', 'volume': '24', 'author': 'Hendrikx', 'year': '1996', 'journal-title': 'Exp Hematol'} / Exp Hematol / Homing of fluorescently labeled murine hematopoietic stem cells. by Hendrikx (1996)
10.1016/0165-4608(92)90345-9 / Cancer Genet Cytogenet / Fluorescence in situ hybridization to determine engraftment status after murine bone marrow transplant. by Hawkins (1992)
10.1002/ajh.2830160309 / Am J Hematol / The total marrow mass of the mouse: A simplified method of measurement. by Boggs (1984)
10.1182/blood.V90.11.4646 / Blood / Synchronized cell-cycle induction of engrafting long-term repopulating stem cells. by Nilsson (1997)
10.1182/blood.V73.2.397.397 / Blood / Two phases of engraftment established by serial bone marrow transplantation in mice. by Jones (1989)
10.1126/science.7528940 / Science / Functional isolation and characterization of human hematopoietic stem cells. by Berardi (1995)
10.1182/blood.V90.2.658 / Blood / Orderly process of sequential cytokine stimulation is required for activation and maximal proliferation of primitive human bone marrow CD34+ hematopoietic progenitor cells residing in G0. by Ladd (1997)
10.1182/blood.V90.11.4384 / Blood / Functional heterogeneity of human CD34+ cells isolated in subcompartments of the G0/G1 phase of the cell cycle. by Gothot (1997)
10.1182/blood.V36.6.772.772 / Blood / A difference between spleen-derived and bone marrow-derived colony-forming units in ability to protect lethally irradiated mice. by Kretchmar (1970)
10.1182/blood.V68.3.787.787 / Blood / Thymic regulation of hematopoiesis III: Isolation of “Helper” and “Suppressor” populations using counterflow centrifugal elutriation (CCE). by Sharkis (1986)
{'key': '2020021216091867400_B17', 'first-page': '223a', 'article-title': 'Thymic regulation of stem cell engraftment.', 'volume': '86', 'author': 'Sharkis', 'year': '1995', 'journal-title': 'Blood'} / Blood / Thymic regulation of stem cell engraftment. by Sharkis (1995)
10.1182/blood.V90.12.4779 / Blood / Antibodies to VLA4 integrin mobilize long-term repopulating cells and augment cytokine-induced mobilization in primates and mice. by Craddock (1997)

Dates

Type	When
Created	5 years, 10 months ago (Oct. 11, 2019, 8:43 a.m.)
Deposited	4 years, 7 months ago (Jan. 24, 2021, 10:12 a.m.)
Indexed	2 weeks ago (Aug. 21, 2025, 1:23 p.m.)
Issued	26 years, 5 months ago (March 15, 1999)
Published	26 years, 5 months ago (March 15, 1999)
Published Print	26 years, 5 months ago (March 15, 1999)

Funders 0

None

BibTeX

@article{Lanzkron_1999, title={Hematopoietic Stem Cell Tracking In Vivo: A Comparison of Short-Term and Long-Term Repopulating Cells}, volume={93}, ISSN={0006-4971}, url={http://dx.doi.org/10.1182/blood.v93.6.1916.406k15_1916_1921}, DOI={10.1182/blood.v93.6.1916.406k15_1916_1921}, number={6}, journal={Blood}, publisher={American Society of Hematology}, author={Lanzkron, Sophie M. and Collector, Michael I. and Sharkis, Saul J.}, year={1999}, month=mar, pages={1916–1921} }

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