DOI: 10.1182/blood.v92.11.4119. Functional Analysis of Mature Hematopoietic Cells From Mice Lacking the βc Chain of the Granulocyte-Macrophage Colony-Stimulating Factor Receptor

Functional Analysis of Mature Hematopoietic Cells From Mice Lacking the βc Chain of the Granulocyte-Macrophage Colony-Stimulating Factor Receptor

10.1182/blood.v92.11.4119

Crossref journal-article

American Society of Hematology

Blood (234)

Abstract

AbstractMice with a null mutation of the βc chain of the granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3), and IL-5 receptors (βc-null mice) develop an alveolar proteinosis-like lung disease. The pathogenesis of this disease is uncertain and, although a defect in alveolar macrophage function has been postulated, no previous analysis of mature hematopoietic cells in mice with alveolar proteinosis has been reported. Therefore, we undertook a functional analysis of the mature hematopoietic cell compartment in βc-null mice. In addition, we reexamined the roles of the GM-CSF receptor  chain and the βc chain in signaling by GM-CSF. Neutrophils and macrophages from βc-null mice were capable of normal survival and phagocytosis in the absence of stimulus and of similar levels of nitric oxide production in response to interferon-γ and lipopolysaccharide. GM-CSF–mediated augmentation of survival, phagocytosis, and hydrogen-ion production were absent in neutrophils from βc-null mice. Interestingly, we were unable to show any ability of the GM-CSF receptor -chain alone to mediate glucose transport in these cells. In keeping with the βc-null mice lung pathology, examination of lavage fluid from the lungs of βc-null mice showed increased cellularity. This was caused by an increase in the number of lymphocytes, neutrophils, and macrophages. Large foamy cells in the lavage fluid from βc-null mice were identified as macrophages using immunohistochemistry. Functional analysis showed that these βc-null alveolar macrophages were capable of phagocytosis but uptake of colloidal carbon and cellular adhesion were reduced. In summary, mature hematopoietic cells with a null mutation of the βc receptor were unable to perform GM-CSF–mediated hematopoietic cell functions including glucose transport, but responded normally to a range of other ligands.

Bibliography

Scott, C. L., Hughes, D. A., Cary, D., Nicola, N. A., Begley, C. G., & Robb, L. (1998). Functional Analysis of Mature Hematopoietic Cells From Mice Lacking the Î²c Chain of the Granulocyte-Macrophage Colony-Stimulating Factor Receptor. Blood, 92(11), 4119â4127.

Authors 6

C.L. Scott (first)
D.A. Hughes (additional)
D. Cary (additional)
N.A. Nicola (additional)
C.G. Begley (additional)
L. Robb (additional)

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Dates

Type	When
Created	5 years, 10 months ago (Oct. 14, 2019, 6:19 a.m.)
Deposited	1 year, 11 months ago (Sept. 21, 2023, 12:48 p.m.)
Indexed	5 months, 1 week ago (March 29, 2025, 6:30 p.m.)
Issued	26 years, 9 months ago (Dec. 1, 1998)
Published	26 years, 9 months ago (Dec. 1, 1998)
Published Print	26 years, 9 months ago (Dec. 1, 1998)

Funders 0

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BibTeX

@article{Scott_1998, title={Functional Analysis of Mature Hematopoietic Cells From Mice Lacking the βc Chain of the Granulocyte-Macrophage Colony-Stimulating Factor Receptor}, volume={92}, ISSN={0006-4971}, url={http://dx.doi.org/10.1182/blood.v92.11.4119}, DOI={10.1182/blood.v92.11.4119}, number={11}, journal={Blood}, publisher={American Society of Hematology}, author={Scott, C.L. and Hughes, D.A. and Cary, D. and Nicola, N.A. and Begley, C.G. and Robb, L.}, year={1998}, month=dec, pages={4119–4127} }

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