DOI: 10.1182/blood.v92.10.3730. Immunostimulatory CpG Oligodeoxynucleotides Enhance the Immune Response to Vaccine Strategies Involving Granulocyte-Macrophage Colony-Stimulating Factor

Immunostimulatory CpG Oligodeoxynucleotides Enhance the Immune Response to Vaccine Strategies Involving Granulocyte-Macrophage Colony-Stimulating Factor

10.1182/blood.v92.10.3730

Crossref journal-article

American Society of Hematology

Blood (234)

Abstract

AbstractImmunostimulatory oligodeoxynucleotides containing the CpG motif (CpG ODN) can activate various immune cell subsets and induce production of a number of cytokines. Prior studies have demonstrated that both CpG ODN and granulocyte-macrophage colony-stimulating factor (GM-CSF) can serve as potent vaccine adjuvants. We used the 38C13 murine lymphoma system to evaluate the immune response to a combination of these two adjuvants. Immunization using antigen, CpG ODN, and soluble GM-CSF enhanced production of antigen-specific antibody and shifted production towards the IgG2a isotype, suggesting an enhanced TH1 response. This effect was most pronounced after repeat immunizations with CpG ODN and antigen/GM-CSF fusion protein. A single immunization with CpG ODN and antigen/GM-CSF fusion protein 3 days before tumor inoculation prevented tumor growth. CpG ODN enhanced the production of interleukin-12 by bone marrow-derived dendritic cells and increased expression of major histocompatibility complex class I and class II molecules, particularly when cells were pulsed with antigen/GM-CSF fusion protein. We conclude that the use of CpG ODN in combination with strategies involving GM-CSF enhances the immune response to antigen and shifts the response towards a TH1 response and that this approach deserves further evaluation in tumor immunization approaches and other conditions in which an antigen-specific TH1 response is desirable.

Bibliography

Liu, H.-M., Newbrough, S. E., Bhatia, S. K., Dahle, C. E., Krieg, A. M., & Weiner, G. J. (1998). Immunostimulatory CpG Oligodeoxynucleotides Enhance the Immune Response to Vaccine Strategies Involving Granulocyte-Macrophage Colony-Stimulating Factor. Blood, 92(10), 3730â3736.

Authors 6

Hsin-Ming Liu (first)
Sally E. Newbrough (additional)
Sudershan K. Bhatia (additional)
Christopher E. Dahle (additional)
Arthur M. Krieg (additional)
George J. Weiner (additional)

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Dates

Type	When
Created	5 years, 10 months ago (Oct. 14, 2019, 9:07 a.m.)
Deposited	1 year, 11 months ago (Sept. 21, 2023, 1 p.m.)
Indexed	2 months, 3 weeks ago (June 3, 2025, 12:30 a.m.)
Issued	26 years, 9 months ago (Nov. 15, 1998)
Published	26 years, 9 months ago (Nov. 15, 1998)
Published Print	26 years, 9 months ago (Nov. 15, 1998)

Funders 0

None

BibTeX

@article{Liu_1998, title={Immunostimulatory CpG Oligodeoxynucleotides Enhance the Immune Response to Vaccine Strategies Involving Granulocyte-Macrophage Colony-Stimulating Factor}, volume={92}, ISSN={0006-4971}, url={http://dx.doi.org/10.1182/blood.v92.10.3730}, DOI={10.1182/blood.v92.10.3730}, number={10}, journal={Blood}, publisher={American Society of Hematology}, author={Liu, Hsin-Ming and Newbrough, Sally E. and Bhatia, Sudershan K. and Dahle, Christopher E. and Krieg, Arthur M. and Weiner, George J.}, year={1998}, month=nov, pages={3730–3736} }

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