DOI: 10.1182/blood.v91.11.4106. Rhodamine-123 Staining in Hematopoietic Stem Cells of Young Mice Indicates Mitochondrial Activation Rather Than Dye Efflux

Rhodamine-123 Staining in Hematopoietic Stem Cells of Young Mice Indicates Mitochondrial Activation Rather Than Dye Efflux

10.1182/blood.v91.11.4106

Crossref journal-article

American Society of Hematology

Blood (234)

Abstract

AbstractLow-intensity fluorescence of rhodamine-123 (Rh-123) discriminates a quiescent hematopoietic stem cell (HSC) population in mouse bone marrow, which provides stable, long-term hematopoiesis after transplantation. Rh-123 labels mitochondria with increasing intensity proportional to cellular activation, however the intensity of staining also correlates with the multidrug resistance (MDR) phenotype, as Rh-123 is a substrate for P-glycoprotein (P-gp). To address the mechanisms of long-term repopulating HSC discrimination by Rh-123, mouse bone marrow stem and progenitor cells were isolated based on surface antigen expression and subsequently separated into subsets using various fluorescent probes sensitive to mitochondrial characteristics and/or MDR function. We determined the cell cycle status of the separated populations and tested for HSC function using transplantation assays. Based on blocking studies using MDR modulators, we observed little efflux of Rh-123 from HSC obtained from young (3- to 4-week-old) mice, but significant efflux from HSC derived from older animals. A fluorescent MDR substrate (Bodipy-verapamil, BodVer) and Rh-123 both segregated quiescent cells into a dim-staining population, however Rh-123–based separations resulted in better enrichment of HSC function. Similar experiments using two other fluorescent probes with specificity for either mitochondrial mass or membrane potential indicated that mitochondrial activation is more important than either mitochondrial mass or MDR function in defining HSC in young mice. This conclusion was supported by morphologic studies of cell subsets separated by Rh-123 staining.

Bibliography

Kim, M., Cooper, D. D., Hayes, S. F., & Spangrude, G. J. (1998). Rhodamine-123 Staining in Hematopoietic Stem Cells of Young Mice Indicates Mitochondrial Activation Rather Than Dye Efflux. Blood, 91(11), 4106â4117.

Authors 4

MiJung Kim (first)
Donna D. Cooper (additional)
Stanley F. Hayes (additional)
Gerald J. Spangrude (additional)

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Dates

Type	When
Created	5 years, 10 months ago (Oct. 14, 2019, 8:57 a.m.)
Deposited	1 year, 11 months ago (Sept. 21, 2023, 12:58 p.m.)
Indexed	1 week, 3 days ago (Aug. 21, 2025, 1:02 p.m.)
Issued	27 years, 3 months ago (June 1, 1998)
Published	27 years, 3 months ago (June 1, 1998)
Published Print	27 years, 3 months ago (June 1, 1998)

Funders 0

None

BibTeX

@article{Kim_1998, title={Rhodamine-123 Staining in Hematopoietic Stem Cells of Young Mice Indicates Mitochondrial Activation Rather Than Dye Efflux}, volume={91}, ISSN={0006-4971}, url={http://dx.doi.org/10.1182/blood.v91.11.4106}, DOI={10.1182/blood.v91.11.4106}, number={11}, journal={Blood}, publisher={American Society of Hematology}, author={Kim, MiJung and Cooper, Donna D. and Hayes, Stanley F. and Spangrude, Gerald J.}, year={1998}, month=jun, pages={4106–4117} }

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