DOI: 10.1182/blood.v89.8.2670. Human Immunodeficiency Virus (HIV)-Resistant CD4+ UT-7 Megakaryocytic Human Cell Line Becomes Highly HIV-1 and HIV-2 Susceptible Upon CXCR4 Transfection: Induction of Cell Differentiation by HIV-1 Infection

Human Immunodeficiency Virus (HIV)-Resistant CD4+ UT-7 Megakaryocytic Human Cell Line Becomes Highly HIV-1 and HIV-2 Susceptible Upon CXCR4 Transfection: Induction of Cell Differentiation by HIV-1 Infection

10.1182/blood.v89.8.2670

Crossref journal-article

American Society of Hematology

Blood (234)

Abstract

AbstractRecent findings have shown that the expression of the seven trans-membrane G-protein–coupled CXCR4 (the receptor for the stromal cell-derived factor [SDF]-1 chemokine) is necessary for the entry of T-lymphotropic human immunodeficiency virus (HIV) strains, acting as a coreceptor of the CD4 molecule. In the human system, the role of CXCR4 in HIV infection has been determined through env-mediated cell fusion assays and confirmed by blocking viral entry in CD4+/CXCR4+ cells by SDF-1 pretreatment. We observed that the human megakaryoblastic CD4+ UT-7 cell line fails to express CXCR4 RNA and is fully resistant to HIV entry. Transfection of an expression vector containing the CXCR4 c-DNA rendered UT-7 cells readily infectable by different T-lymphotropic syncytium-inducing HIV-1 and HIV-2 isolates. Interestingly, HIV-1 infection of CXCR4 expressing UT-7 cells (named UT-7/fus) induces the formation of polynucleated cells through a process highly reminiscent of megakaryocytic differentiation and maturation. On the contrary, no morphologic changes were observed in HIV-2–infected UT-7/fus cells. These findings further strengthen the role of CXCR4 as a molecule necessary for the replication of T-lymphotropic HIV-1 and HIV-2 isolates and provide a useful model to study the functional role of CD4 coreceptors in HIV infection.

Bibliography

Baiocchi, M., Olivetta, E., Chelucci, C., Santarcangelo, A. C., Bona, R., dâAloja, P., Testa, U., Komatsu, N., Verani, P., & Federico, M. (1997). Human Immunodeficiency Virus (HIV)-Resistant CD4+ UT-7 Megakaryocytic Human Cell Line Becomes Highly HIV-1 and HIV-2 Susceptible Upon CXCR4 Transfection: Induction of Cell Differentiation by HIV-1 Infection. Blood, 89(8), 2670â2678.

Authors 10

Marta Baiocchi (first)
Eleonora Olivetta (additional)
Cristiana Chelucci (additional)
Anna Claudia Santarcangelo (additional)
Roberta Bona (additional)
Paola d'Aloja (additional)
Ugo Testa (additional)
Norio Komatsu (additional)
Paola Verani (additional)
Maurizio Federico (additional)

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{'key': '2019111909263895900_B1'}
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Dates

Type	When
Created	5 years, 10 months ago (Oct. 12, 2019, 3:35 p.m.)
Deposited	4 years, 7 months ago (Jan. 24, 2021, 12:33 p.m.)
Indexed	1 year ago (Aug. 8, 2024, 4:36 p.m.)
Issued	28 years, 4 months ago (April 15, 1997)
Published	28 years, 4 months ago (April 15, 1997)
Published Print	28 years, 4 months ago (April 15, 1997)

Funders 0

None

BibTeX

@article{Baiocchi_1997, title={Human Immunodeficiency Virus (HIV)-Resistant CD4+ UT-7 Megakaryocytic Human Cell Line Becomes Highly HIV-1 and HIV-2 Susceptible Upon CXCR4 Transfection: Induction of Cell Differentiation by HIV-1 Infection}, volume={89}, ISSN={0006-4971}, url={http://dx.doi.org/10.1182/blood.v89.8.2670}, DOI={10.1182/blood.v89.8.2670}, number={8}, journal={Blood}, publisher={American Society of Hematology}, author={Baiocchi, Marta and Olivetta, Eleonora and Chelucci, Cristiana and Santarcangelo, Anna Claudia and Bona, Roberta and d’Aloja, Paola and Testa, Ugo and Komatsu, Norio and Verani, Paola and Federico, Maurizio}, year={1997}, month=apr, pages={2670–2678} }

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