DOI: 10.1182/blood.v89.6.2113. Sorting of the Specific Granule Protein, NGAL, During Granulocytic Maturation of HL-60 Cells

Sorting of the Specific Granule Protein, NGAL, During Granulocytic Maturation of HL-60 Cells

10.1182/blood.v89.6.2113

Crossref journal-article

American Society of Hematology

Blood (234)

Abstract

AbstractThe different types of human neutrophil granules (azurophil, specific, and gelatinase granules) are formed sequentially during maturation of neutrophils from the promyelocyte stage to the band cell stage. The promyelocytic HL-60 cells can maturate to segmented granulocytes but are incapable of activating the transcription of any known intragranular protein, normally located in specific or gelatinase granules. To study the sorting of granule proteins during maturation, we transfected HL-60 cells with the specific granule protein NGAL, inserted under control of a cytomegalovirus promoter. We previously showed that NGAL is sorted to azurophil granules and colocalizes with myeloperoxidase in undifferentiated HL-60 cells. We show here that, when such transfected HL-60 cells differentiate into granulocytes, newly synthesized NGAL is not retained in granules but is constitutively secreted. This indicates that highly specific mechanisms must exist that are responsible for diverting transport vesicles into storage granules, and that HL-60 cells not only lack the ability to activate transcription of specific granule proteins, but also lose the ability to form storage granules during maturation.

Bibliography

Le Cabec, V., Calafat, J., & Borregaard, N. (1997). Sorting of the Specific Granule Protein, NGAL, During Granulocytic Maturation of HL-60 Cells. Blood, 89(6), 2113â2121.

Authors 3

Véronique Le Cabec (first)
Jero Calafat (additional)
Niels Borregaard (additional)

References 43 Referenced 43

10.1111/j.1600-0609.1993.tb00629.x / Eur J Haematol / Human neutrophil granules and secretory vesicles. by Borregaard (1993)
10.1084/jem.134.4.907 / J Exp Med / The development of neutrophilic polymorphonuclear leukocytes in human bone marrow. by Bainton (1971)
10.1182/blood.V70.3.757.bloodjournal703757 / Blood / Defensin-rich dense granules of human neutrophils. by Rice (1987)
10.1182/blood.V82.10.3183.3183 / Blood / Structural and functional heterogeneity among peroxidase-negative granules in human neutrophils: Identification of a distinct gelatinase containing granule subset by combined immunocytochemistry and subcellular fractionation. by Kjeldsen (1993)
10.1172/JCI110643 / J Clin Invest / Release of gelatinase from a novel secretory compartment of human neutrophils. by Dewald (1982)
10.4049/jimmunol.150.4.1535 / J Immunol / Control of exocytosis in early neutrophil activation. by Sengeløv (1993)
10.4049/jimmunol.154.8.4157 / J Immunol / Mobilization of granules and secretory vesicles during in vivo exudation of human neutrophils. by Sengeløv (1995)
10.1182/blood.V85.3.812.bloodjournal853812 / Blood / Biosynthesis of granule proteins in normal human bone marrow cells. Gelatinase is a marker of terminal neutrophil differentiation. by Borregaard (1995)
10.1097/00062752-199603010-00003 / Cur Opin Hematol / Current opinion about neutrophil granule physiology. by Borregaard (1996)
10.1111/j.1365-2141.1975.tb01795.x / Br J Haematol / Neutrophil granules. by Bainton (1975)
10.1016/S0021-9258(18)82217-7 / J Biol Chem / Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase. by Kjeldsen (1993)
10.1182/blood.V83.3.799.799 / Blood / Identification of NGAL as a novel matrix protein of specific granules in human neutrophils. by Kjeldsen (1994)
10.1073/pnas.93.13.6454 / Proc Natl Acad Sci USA / Targeting of proteins to granule subsets determined by timing not by sorting: The specific granule protein NGAL is localized to azurophil granules when expressed in HL-60 cells. by Le Cabec (1996)
10.1016/S0021-9258(17)43161-9 / J Biol Chem / Development of the superoxide-generating system during differentiation of the HL-60 human promyelocytic leukemia cell line. by Newburger (1984)
10.1128/MCB.8.7.2804 / Mol Cell Biol / Expression of the X-CGD gene during induced differentiation of myeloid leukemia cell line HL-60. by Barker (1988)
10.1182/blood.V73.1.131.131 / Blood / Differential expression of CD11b/CD18 (Mo1) and myeloperoxidase genes during myeloid differentiation. by Rosmarin (1989)
10.1084/jem.166.6.1641 / J Exp Med / Leukocyte adhesion receptors are stored in peroxidase-negative granules of human neutrophils. by Bainton (1987)
10.1172/JCI116724 / J Clin Invest / Subcellular localization and dynamics of Mac-1 (αmβ2 ) in human neutrophils. by Sengeløv (1993)
10.1182/blood.V79.11.2998.2998 / Blood / Lactoferrin gene promoter: Structual integrity and nonexpression in HL60 cells. by Johnston (1992)
10.1182/blood.V70.5.1233.bloodjournal7051233 / Blood / The HL-60 promyelocytic leukemia cell line: Proliferation, differentiation, and cellular oncogene expression. by Collins (1987)
10.1182/blood.V62.2.308.bloodjournal622308 / Blood / Changes in actin content during induced myeloid maturation of human promyelocytes. by Meyer (1983)
10.1182/blood.V87.1.350.350 / Blood / Distinct temporal patterns of defensin mRNA regulation during drug-induced differentiation of human myeloid leukemia cells. by Herwig (1996)
10.1002/jlb.56.1.80 / J Leuk Biol / Changes in the subcellular localization and surface expression of L-selectin, alkaline phosphatase, and Mac-1 in human neutrophils during stimulation with inflammatory mediators. by Borregaard (1994)
10.1016/0003-9861(61)90009-1 / Arch Biochem Biophys / Measurement of the incorporation of radioactive amino acids into proteins by a filter-paper disk method. by Mans (1961)
10.1038/227680a0 / Nature / Cleavage of structural proteins during the assembly of the head of bacteriophage T4. by Laemmli (1970)
10.1042/bj2230911 / Biochem J / Biosynthesis,transport and processing of myeloperoxidase in the human leukaemic promyelocytic cell line HL-60 and normal marrow cells. by Olsson (1984)
10.1172/JCI115860 / J Clin Invest / Stimulus-dependent secretion of plasma proteins from human neutrophils. by Borregaard (1992)
10.1182/blood.V73.6.1686.1686 / Blood / Characterization of two monoclonal antibodies against cytochrome b558 of human neutrophils. by Verhoeven (1989)
10.1016/0003-9861(88)90411-0 / Arch Biochem Biophys / Transcriptional regulation of myeloperoxidase gene expression in myeloid leukemia HL-60 cells during differentiation into granulocytes and macrophages. by Sagoh (1988)
10.1016/0092-8674(91)90648-I / Cell / Induction of specific storage organelles by von Willebrand factor propolypeptide. by Wagner (1991)
10.1073/pnas.92.19.8655 / Proc Natl Acad Sci USA / De novo formation of caveolae in lymphocytes by expression of VIP21-caveolin. by Fra (1995)
10.1073/pnas.92.16.7450 / Proc Natl Acad Sci USA / Platelets roll on stimulated endothelium in vivo: An interaction mediated by endothelial p-selectin. by Frenette (1995)
10.1172/JCI114509 / J Clin Invest / Ultrastructual localization of cytochrome b in the membranes of resting and phagocytosing human granulocytes. by Jesaitis (1990)
10.1083/jcb.97.1.52 / J Cell Biol / Subcellular localization of the b-cytochrome component of the human neutrophil microbicidal oxidase: Translocation during activation. by Borregaard (1983)
10.1182/blood.V83.6.1640.1640 / Blood / Isolation and characterization of gelatinase granules from human neutrophils. by Kjeldsen (1994)
10.1146/annurev.bi.61.070192.002351 / Annu Rev Biochem / Vesicle-mediated protein sorting. by Pryer (1992)
{'key': '2019111910041767100_B37', 'first-page': '424', 'article-title': 'The processing and intracellular transport of myeloperoxidase. Modulation by lysosomotropic agents and monensin.', 'volume': '39', 'author': 'Strömberg', 'year': '1985', 'journal-title': 'Eur J Cell Biol'} / Eur J Cell Biol / The processing and intracellular transport of myeloperoxidase. Modulation by lysosomotropic agents and monensin. by Strömberg (1985)
10.1182/blood.V68.2.442.bloodjournal682442 / Blood / Separation and analysis of subcellular organelles in a human promyelocytic leukemia cell line, HL-60: Application to the study of myeloid lysosomal enzyme synthesis and processing. by Nauseef (1986)
10.1182/blood.V67.4.865.865 / Blood / Myeloperoxidase biosynthesis by a human promyelocytic leukemia cell line: Insight into myeloperoxidase deficiency. by Nauseef (1986)
10.1182/blood.V79.6.1538.1538 / Blood / Posttranslational processing of defensins in immature human myeloid cells. by Valore (1992)
10.1182/blood.V76.11.2374.2374 / Blood / Biosynthesis and processing of cathepsin G and neutrophil elastase in the leukemic myeloid cell line U-937. by Lindmark (1990)
{'key': '2019111910041767100_B42'}
10.1016/S0021-9258(18)45970-4 / J Biol Chem / Regulated and constitutive secretion. Differential effects of protein synthesis arrest on transport of glycosaminoglycan chains to the two secretory pathways. by Brion (1992)

Dates

Type	When
Created	5 years, 10 months ago (Oct. 12, 2019, 5:19 p.m.)
Deposited	1 year, 11 months ago (Sept. 21, 2023, 11:45 a.m.)
Indexed	1 year, 2 months ago (June 16, 2024, 3:50 a.m.)
Issued	28 years, 5 months ago (March 15, 1997)
Published	28 years, 5 months ago (March 15, 1997)
Published Print	28 years, 5 months ago (March 15, 1997)

Funders 0

None

BibTeX

@article{Le_Cabec_1997, title={Sorting of the Specific Granule Protein, NGAL, During Granulocytic Maturation of HL-60 Cells}, volume={89}, ISSN={0006-4971}, url={http://dx.doi.org/10.1182/blood.v89.6.2113}, DOI={10.1182/blood.v89.6.2113}, number={6}, journal={Blood}, publisher={American Society of Hematology}, author={Le Cabec, Véronique and Calafat, Jero and Borregaard, Niels}, year={1997}, month=mar, pages={2113–2121} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        6,
        16
      ]
    ],
    "date-time": "2024-06-16T07:50:19Z",
    "timestamp": 1718524219974
  },
  "reference-count": 43,
  "publisher": "American Society of Hematology",
  "issue": "6",
  "content-domain": {
    "domain": [
      "ashpublications.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1997,
        3,
        15
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>The different types of human neutrophil granules (azurophil, specific, and gelatinase granules) are formed sequentially during maturation of neutrophils from the promyelocyte stage to the band cell stage. The promyelocytic HL-60 cells can maturate to segmented granulocytes but are incapable of activating the transcription of any known intragranular protein, normally located in specific or gelatinase granules. To study the sorting of granule proteins during maturation, we transfected HL-60 cells with the specific granule protein NGAL, inserted under control of a cytomegalovirus promoter. We previously showed that NGAL is sorted to azurophil granules and colocalizes with myeloperoxidase in undifferentiated HL-60 cells. We show here that, when such transfected HL-60 cells differentiate into granulocytes, newly synthesized NGAL is not retained in granules but is constitutively secreted. This indicates that highly specific mechanisms must exist that are responsible for diverting transport vesicles into storage granules, and that HL-60 cells not only lack the ability to activate transcription of specific granule proteins, but also lose the ability to form storage granules during maturation.</jats:p>",
  "DOI": "10.1182/blood.v89.6.2113",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2019,
        10,
        12
      ]
    ],
    "date-time": "2019-10-12T21:19:17Z",
    "timestamp": 1570915157000
  },
  "page": "2113-2121",
  "update-policy": "http://dx.doi.org/10.1182/blood.2019cm0000",
  "source": "Crossref",
  "is-referenced-by-count": 43,
  "title": "Sorting of the Specific Granule Protein, NGAL, During Granulocytic Maturation of HL-60 Cells",
  "prefix": "10.1182",
  "volume": "89",
  "author": [
    {
      "given": "Ve\u0301ronique",
      "family": "Le Cabec",
      "sequence": "first",
      "affiliation": [
        {
          "name": "From The Granulocyte Research Laboratory, Department of Hematology, Finsen Center, The National University Hospital, Rigshospitalet, Copenhagen, Denmark; and The Netherlands Cancer Institute, Amsterdam, The Netherlands."
        }
      ]
    },
    {
      "given": "Jero",
      "family": "Calafat",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "From The Granulocyte Research Laboratory, Department of Hematology, Finsen Center, The National University Hospital, Rigshospitalet, Copenhagen, Denmark; and The Netherlands Cancer Institute, Amsterdam, The Netherlands."
        }
      ]
    },
    {
      "given": "Niels",
      "family": "Borregaard",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "From The Granulocyte Research Laboratory, Department of Hematology, Finsen Center, The National University Hospital, Rigshospitalet, Copenhagen, Denmark; and The Netherlands Cancer Institute, Amsterdam, The Netherlands."
        }
      ]
    }
  ],
  "member": "234",
  "reference": [
    {
      "key": "2019111910041767100_B1",
      "doi-asserted-by": "crossref",
      "first-page": "187",
      "DOI": "10.1111/j.1600-0609.1993.tb00629.x",
      "article-title": "Human neutrophil granules and secretory vesicles.",
      "volume": "51",
      "author": "Borregaard",
      "year": "1993",
      "journal-title": "Eur J Haematol"
    },
    {
      "key": "2019111910041767100_B2",
      "doi-asserted-by": "crossref",
      "first-page": "907",
      "DOI": "10.1084/jem.134.4.907",
      "article-title": "The development of neutrophilic polymorphonuclear leukocytes in human bone marrow.",
      "volume": "143",
      "author": "Bainton",
      "year": "1971",
      "journal-title": "J Exp Med"
    },
    {
      "key": "2019111910041767100_B3",
      "doi-asserted-by": "crossref",
      "first-page": "757",
      "DOI": "10.1182/blood.V70.3.757.bloodjournal703757",
      "article-title": "Defensin-rich dense granules of human neutrophils.",
      "volume": "70",
      "author": "Rice",
      "year": "1987",
      "journal-title": "Blood"
    },
    {
      "key": "2019111910041767100_B4",
      "doi-asserted-by": "crossref",
      "first-page": "3183",
      "DOI": "10.1182/blood.V82.10.3183.3183",
      "article-title": "Structural and functional heterogeneity among peroxidase-negative granules in human neutrophils: Identification of a distinct gelatinase containing granule subset by combined immunocytochemistry and subcellular fractionation.",
      "volume": "82",
      "author": "Kjeldsen",
      "year": "1993",
      "journal-title": "Blood"
    },
    {
      "key": "2019111910041767100_B5",
      "doi-asserted-by": "crossref",
      "first-page": "518",
      "DOI": "10.1172/JCI110643",
      "article-title": "Release of gelatinase from a novel secretory compartment of human neutrophils.",
      "volume": "70",
      "author": "Dewald",
      "year": "1982",
      "journal-title": "J Clin Invest"
    },
    {
      "key": "2019111910041767100_B6",
      "doi-asserted-by": "crossref",
      "first-page": "1535",
      "DOI": "10.4049/jimmunol.150.4.1535",
      "article-title": "Control of exocytosis in early neutrophil activation.",
      "volume": "150",
      "author": "Sengel\u00f8v",
      "year": "1993",
      "journal-title": "J Immunol"
    },
    {
      "key": "2019111910041767100_B7",
      "doi-asserted-by": "crossref",
      "first-page": "4157",
      "DOI": "10.4049/jimmunol.154.8.4157",
      "article-title": "Mobilization of granules and secretory vesicles during in vivo exudation of human neutrophils.",
      "volume": "154",
      "author": "Sengel\u00f8v",
      "year": "1995",
      "journal-title": "J Immunol"
    },
    {
      "key": "2019111910041767100_B8",
      "doi-asserted-by": "crossref",
      "first-page": "812",
      "DOI": "10.1182/blood.V85.3.812.bloodjournal853812",
      "article-title": "Biosynthesis of granule proteins in normal human bone marrow cells. Gelatinase is a marker of terminal neutrophil differentiation.",
      "volume": "85",
      "author": "Borregaard",
      "year": "1995",
      "journal-title": "Blood"
    },
    {
      "key": "2019111910041767100_B9",
      "doi-asserted-by": "crossref",
      "first-page": "11",
      "DOI": "10.1097/00062752-199603010-00003",
      "article-title": "Current opinion about neutrophil granule physiology.",
      "volume": "3",
      "author": "Borregaard",
      "year": "1996",
      "journal-title": "Cur Opin Hematol"
    },
    {
      "key": "2019111910041767100_B10",
      "doi-asserted-by": "crossref",
      "first-page": "17",
      "DOI": "10.1111/j.1365-2141.1975.tb01795.x",
      "article-title": "Neutrophil granules.",
      "volume": "29",
      "author": "Bainton",
      "year": "1975",
      "journal-title": "Br J Haematol"
    },
    {
      "key": "2019111910041767100_B11",
      "doi-asserted-by": "crossref",
      "first-page": "10425",
      "DOI": "10.1016/S0021-9258(18)82217-7",
      "article-title": "Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase.",
      "volume": "268",
      "author": "Kjeldsen",
      "year": "1993",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "2019111910041767100_B12",
      "doi-asserted-by": "crossref",
      "first-page": "799",
      "DOI": "10.1182/blood.V83.3.799.799",
      "article-title": "Identification of NGAL as a novel matrix protein of specific granules in human neutrophils.",
      "volume": "83",
      "author": "Kjeldsen",
      "year": "1994",
      "journal-title": "Blood"
    },
    {
      "key": "2019111910041767100_B13",
      "doi-asserted-by": "crossref",
      "first-page": "6454",
      "DOI": "10.1073/pnas.93.13.6454",
      "article-title": "Targeting of proteins to granule subsets determined by timing not by sorting: The specific granule protein NGAL is localized to azurophil granules when expressed in HL-60 cells.",
      "volume": "93",
      "author": "Le Cabec",
      "year": "1996",
      "journal-title": "Proc Natl Acad Sci USA"
    },
    {
      "key": "2019111910041767100_B14",
      "doi-asserted-by": "crossref",
      "first-page": "3771",
      "DOI": "10.1016/S0021-9258(17)43161-9",
      "article-title": "Development of the superoxide-generating system during differentiation of the HL-60 human promyelocytic leukemia cell line.",
      "volume": "259",
      "author": "Newburger",
      "year": "1984",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "2019111910041767100_B15",
      "doi-asserted-by": "crossref",
      "first-page": "2804",
      "DOI": "10.1128/MCB.8.7.2804",
      "article-title": "Expression of the X-CGD gene during induced differentiation of myeloid leukemia cell line HL-60.",
      "volume": "8",
      "author": "Barker",
      "year": "1988",
      "journal-title": "Mol Cell Biol"
    },
    {
      "key": "2019111910041767100_B16",
      "doi-asserted-by": "crossref",
      "first-page": "131",
      "DOI": "10.1182/blood.V73.1.131.131",
      "article-title": "Differential expression of CD11b/CD18 (Mo1) and myeloperoxidase genes during myeloid differentiation.",
      "volume": "73",
      "author": "Rosmarin",
      "year": "1989",
      "journal-title": "Blood"
    },
    {
      "key": "2019111910041767100_B17",
      "doi-asserted-by": "crossref",
      "first-page": "1641",
      "DOI": "10.1084/jem.166.6.1641",
      "article-title": "Leukocyte adhesion receptors are stored in peroxidase-negative granules of human neutrophils.",
      "volume": "166",
      "author": "Bainton",
      "year": "1987",
      "journal-title": "J Exp Med"
    },
    {
      "key": "2019111910041767100_B18",
      "doi-asserted-by": "crossref",
      "first-page": "1467",
      "DOI": "10.1172/JCI116724",
      "article-title": "Subcellular localization and dynamics of Mac-1 (\u03b1m\u03b22 ) in human neutrophils.",
      "volume": "92",
      "author": "Sengel\u00f8v",
      "year": "1993",
      "journal-title": "J Clin Invest"
    },
    {
      "key": "2019111910041767100_B19",
      "doi-asserted-by": "crossref",
      "first-page": "2998",
      "DOI": "10.1182/blood.V79.11.2998.2998",
      "article-title": "Lactoferrin gene promoter: Structual integrity and nonexpression in HL60 cells.",
      "volume": "79",
      "author": "Johnston",
      "year": "1992",
      "journal-title": "Blood"
    },
    {
      "key": "2019111910041767100_B20",
      "doi-asserted-by": "crossref",
      "first-page": "1233",
      "DOI": "10.1182/blood.V70.5.1233.bloodjournal7051233",
      "article-title": "The HL-60 promyelocytic leukemia cell line: Proliferation, differentiation, and cellular oncogene expression.",
      "volume": "70",
      "author": "Collins",
      "year": "1987",
      "journal-title": "Blood"
    },
    {
      "key": "2019111910041767100_B21",
      "doi-asserted-by": "crossref",
      "first-page": "308",
      "DOI": "10.1182/blood.V62.2.308.bloodjournal622308",
      "article-title": "Changes in actin content during induced myeloid maturation of human promyelocytes.",
      "volume": "62",
      "author": "Meyer",
      "year": "1983",
      "journal-title": "Blood"
    },
    {
      "key": "2019111910041767100_B22",
      "doi-asserted-by": "crossref",
      "first-page": "350",
      "DOI": "10.1182/blood.V87.1.350.350",
      "article-title": "Distinct temporal patterns of defensin mRNA regulation during drug-induced differentiation of human myeloid leukemia cells.",
      "volume": "87",
      "author": "Herwig",
      "year": "1996",
      "journal-title": "Blood"
    },
    {
      "key": "2019111910041767100_B23",
      "doi-asserted-by": "crossref",
      "first-page": "80",
      "DOI": "10.1002/jlb.56.1.80",
      "article-title": "Changes in the subcellular localization and surface expression of L-selectin, alkaline phosphatase, and Mac-1 in human neutrophils during stimulation with inflammatory mediators.",
      "volume": "56",
      "author": "Borregaard",
      "year": "1994",
      "journal-title": "J Leuk Biol"
    },
    {
      "key": "2019111910041767100_B24",
      "doi-asserted-by": "crossref",
      "first-page": "48",
      "DOI": "10.1016/0003-9861(61)90009-1",
      "article-title": "Measurement of the incorporation of radioactive amino acids into proteins by a filter-paper disk method.",
      "volume": "94",
      "author": "Mans",
      "year": "1961",
      "journal-title": "Arch Biochem Biophys"
    },
    {
      "key": "2019111910041767100_B25",
      "doi-asserted-by": "crossref",
      "first-page": "680",
      "DOI": "10.1038/227680a0",
      "article-title": "Cleavage of structural proteins during the assembly of the head of bacteriophage T4.",
      "volume": "227",
      "author": "Laemmli",
      "year": "1970",
      "journal-title": "Nature"
    },
    {
      "key": "2019111910041767100_B26",
      "doi-asserted-by": "crossref",
      "first-page": "911",
      "DOI": "10.1042/bj2230911",
      "article-title": "Biosynthesis,transport and processing of myeloperoxidase in the human leukaemic promyelocytic cell line HL-60 and normal marrow cells.",
      "volume": "223",
      "author": "Olsson",
      "year": "1984",
      "journal-title": "Biochem J"
    },
    {
      "key": "2019111910041767100_B27",
      "doi-asserted-by": "crossref",
      "first-page": "86",
      "DOI": "10.1172/JCI115860",
      "article-title": "Stimulus-dependent secretion of plasma proteins from human neutrophils.",
      "volume": "90",
      "author": "Borregaard",
      "year": "1992",
      "journal-title": "J Clin Invest"
    },
    {
      "key": "2019111910041767100_B28",
      "doi-asserted-by": "crossref",
      "first-page": "1686",
      "DOI": "10.1182/blood.V73.6.1686.1686",
      "article-title": "Characterization of two monoclonal antibodies against cytochrome b558 of human neutrophils.",
      "volume": "73",
      "author": "Verhoeven",
      "year": "1989",
      "journal-title": "Blood"
    },
    {
      "key": "2019111910041767100_B29",
      "doi-asserted-by": "crossref",
      "first-page": "599",
      "DOI": "10.1016/0003-9861(88)90411-0",
      "article-title": "Transcriptional regulation of myeloperoxidase gene expression in myeloid leukemia HL-60 cells during differentiation into granulocytes and macrophages.",
      "volume": "262",
      "author": "Sagoh",
      "year": "1988",
      "journal-title": "Arch Biochem Biophys"
    },
    {
      "key": "2019111910041767100_B30",
      "doi-asserted-by": "crossref",
      "first-page": "403",
      "DOI": "10.1016/0092-8674(91)90648-I",
      "article-title": "Induction of specific storage organelles by von Willebrand factor propolypeptide.",
      "volume": "64",
      "author": "Wagner",
      "year": "1991",
      "journal-title": "Cell"
    },
    {
      "key": "2019111910041767100_B31",
      "doi-asserted-by": "crossref",
      "first-page": "8655",
      "DOI": "10.1073/pnas.92.19.8655",
      "article-title": "De novo formation of caveolae in lymphocytes by expression of VIP21-caveolin.",
      "volume": "92",
      "author": "Fra",
      "year": "1995",
      "journal-title": "Proc Natl Acad Sci USA"
    },
    {
      "key": "2019111910041767100_B32",
      "doi-asserted-by": "crossref",
      "first-page": "7450",
      "DOI": "10.1073/pnas.92.16.7450",
      "article-title": "Platelets roll on stimulated endothelium in vivo: An interaction mediated by endothelial p-selectin.",
      "volume": "92",
      "author": "Frenette",
      "year": "1995",
      "journal-title": "Proc Natl Acad Sci USA"
    },
    {
      "key": "2019111910041767100_B33",
      "doi-asserted-by": "crossref",
      "first-page": "821",
      "DOI": "10.1172/JCI114509",
      "article-title": "Ultrastructual localization of cytochrome b in the membranes of resting and phagocytosing human granulocytes.",
      "volume": "85",
      "author": "Jesaitis",
      "year": "1990",
      "journal-title": "J Clin Invest"
    },
    {
      "key": "2019111910041767100_B34",
      "doi-asserted-by": "crossref",
      "first-page": "52",
      "DOI": "10.1083/jcb.97.1.52",
      "article-title": "Subcellular localization of the b-cytochrome component of the human neutrophil microbicidal oxidase: Translocation during activation.",
      "volume": "97",
      "author": "Borregaard",
      "year": "1983",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "2019111910041767100_B35",
      "doi-asserted-by": "crossref",
      "first-page": "1640",
      "DOI": "10.1182/blood.V83.6.1640.1640",
      "article-title": "Isolation and characterization of gelatinase granules from human neutrophils.",
      "volume": "83",
      "author": "Kjeldsen",
      "year": "1994",
      "journal-title": "Blood"
    },
    {
      "key": "2019111910041767100_B36",
      "doi-asserted-by": "crossref",
      "first-page": "471",
      "DOI": "10.1146/annurev.bi.61.070192.002351",
      "article-title": "Vesicle-mediated protein sorting.",
      "volume": "61",
      "author": "Pryer",
      "year": "1992",
      "journal-title": "Annu Rev Biochem"
    },
    {
      "key": "2019111910041767100_B37",
      "first-page": "424",
      "article-title": "The processing and intracellular transport of myeloperoxidase. Modulation by lysosomotropic agents and monensin.",
      "volume": "39",
      "author": "Stro\u0308mberg",
      "year": "1985",
      "journal-title": "Eur J Cell Biol"
    },
    {
      "key": "2019111910041767100_B38",
      "doi-asserted-by": "crossref",
      "first-page": "442",
      "DOI": "10.1182/blood.V68.2.442.bloodjournal682442",
      "article-title": "Separation and analysis of subcellular organelles in a human promyelocytic leukemia cell line, HL-60: Application to the study of myeloid lysosomal enzyme synthesis and processing.",
      "volume": "68",
      "author": "Nauseef",
      "year": "1986",
      "journal-title": "Blood"
    },
    {
      "key": "2019111910041767100_B39",
      "doi-asserted-by": "crossref",
      "first-page": "865",
      "DOI": "10.1182/blood.V67.4.865.865",
      "article-title": "Myeloperoxidase biosynthesis by a human promyelocytic leukemia cell line: Insight into myeloperoxidase deficiency.",
      "volume": "67",
      "author": "Nauseef",
      "year": "1986",
      "journal-title": "Blood"
    },
    {
      "key": "2019111910041767100_B40",
      "doi-asserted-by": "crossref",
      "first-page": "1538",
      "DOI": "10.1182/blood.V79.6.1538.1538",
      "article-title": "Posttranslational processing of defensins in immature human myeloid cells.",
      "volume": "79",
      "author": "Valore",
      "year": "1992",
      "journal-title": "Blood"
    },
    {
      "key": "2019111910041767100_B41",
      "doi-asserted-by": "crossref",
      "first-page": "2374",
      "DOI": "10.1182/blood.V76.11.2374.2374",
      "article-title": "Biosynthesis and processing of cathepsin G and neutrophil elastase in the leukemic myeloid cell line U-937.",
      "volume": "76",
      "author": "Lindmark",
      "year": "1990",
      "journal-title": "Blood"
    },
    {
      "key": "2019111910041767100_B42"
    },
    {
      "key": "2019111910041767100_B43",
      "doi-asserted-by": "crossref",
      "first-page": "1477",
      "DOI": "10.1016/S0021-9258(18)45970-4",
      "article-title": "Regulated and constitutive secretion. Differential effects of protein synthesis arrest on transport of glycosaminoglycan chains to the two secretory pathways.",
      "volume": "267",
      "author": "Brion",
      "year": "1992",
      "journal-title": "J Biol Chem"
    }
  ],
  "container-title": "Blood",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "http://ashpublications.org/blood/article-pdf/89/6/2113/1410154/2113.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "http://ashpublications.org/blood/article-pdf/89/6/2113/1410154/2113.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        9,
        21
      ]
    ],
    "date-time": "2023-09-21T15:45:20Z",
    "timestamp": 1695311120000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://ashpublications.org/blood/article/89/6/2113/139116/Sorting-of-the-Specific-Granule-Protein-NGAL"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1997,
        3,
        15
      ]
    ]
  },
  "references-count": 43,
  "journal-issue": {
    "issue": "6",
    "published-print": {
      "date-parts": [
        [
          1997,
          3,
          15
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1182/blood.V89.6.2113",
  "relation": {},
  "ISSN": [
    "1528-0020",
    "0006-4971"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1997,
        3,
        15
      ]
    ]
  }
}