DOI: 10.1182/blood.v66.3.731.731. Glycoprotein IIb-IIIa complex and Ca2+ influx into stimulated platelets

Glycoprotein IIb-IIIa complex and Ca2+ influx into stimulated platelets

10.1182/blood.v66.3.731.731

Crossref journal-article

American Society of Hematology

Blood (234)

Abstract

Abstract Changes in intracellular Ca2+ concentrations [( Ca2+]i) in platelets stimulated with aggregating agents were measured with the fluorescent indicator dye quin 2. Ca2+ influx, but not intracellular mobilization, in response to adenosine diphosphate (ADP), platelet aggregating factor (PAF-acether), and sodium arachidonate was significantly inhibited by monoclonal antibodies against the glycoprotein (GP) IIb-IIIa complex; inhibition of thrombin-stimulated influx was inhibited to a lesser extent and reached statistical significance only at thrombin concentrations of 0.1 U/mL and below. Anti-GP Ib and HLA-ABC monoclonal antibodies had no effect on Ca2+ influx in response to any agonist. Thrombasthenic platelets gave normal [Ca2+]i responses to ADP and thrombin, which were not inhibited by an anti-GP IIb-IIIa antibody. It is suggested that Ca2+ influx in response to weak agonists occurs predominantly via a channel closely adjacent to the GP IIb-IIIa complex, but that higher concentrations of thrombin and A23187 also stimulate influx via another pathway.Changes in intracellular Ca2+ concentrations [( Ca2+]i) in platelets stimulated with aggregating agents were measured with the fluorescent indicator dye quin 2. Ca2+ influx, but not intracellular mobilization, in response to adenosine diphosphate (ADP), platelet aggregating factor (PAF-acether), and sodium arachidonate was significantly inhibited by monoclonal antibodies against the glycoprotein (GP) IIb-IIIa complex; inhibition of thrombin-stimulated influx was inhibited to a lesser extent and reached statistical significance only at thrombin concentrations of 0.1 U/mL and below. Anti-GP Ib and HLA-ABC monoclonal antibodies had no effect on Ca2+ influx in response to any agonist. Thrombasthenic platelets gave normal [Ca2+]i responses to ADP and thrombin, which were not inhibited by an anti-GP IIb-IIIa antibody. It is suggested that Ca2+ influx in response to weak agonists occurs predominantly via a channel closely adjacent to the GP IIb-IIIa complex, but that higher concentrations of thrombin and A23187 also stimulate influx via another pathway.

Bibliography

Powling, M., & Hardisty, R. (1985). Glycoprotein IIb-IIIa complex and Ca2+ influx into stimulated platelets. Blood, 66(3), 731â734.

Authors 2

MJ Powling (first)
RM Hardisty (additional)

References 0 Referenced 55

None

Dates

Type	When
Created	5 years, 10 months ago (Oct. 13, 2019, 8:46 a.m.)
Deposited	5 years, 6 months ago (Feb. 12, 2020, 9:46 p.m.)
Indexed	1 year, 3 months ago (May 12, 2024, 12:28 p.m.)
Issued	40 years ago (Sept. 1, 1985)
Published	40 years ago (Sept. 1, 1985)
Published Print	40 years ago (Sept. 1, 1985)

Funders 0

None

BibTeX

@article{Powling_1985, title={Glycoprotein IIb-IIIa complex and Ca2+ influx into stimulated platelets}, volume={66}, ISSN={1528-0020}, url={http://dx.doi.org/10.1182/blood.v66.3.731.731}, DOI={10.1182/blood.v66.3.731.731}, number={3}, journal={Blood}, publisher={American Society of Hematology}, author={Powling, MJ and Hardisty, RM}, year={1985}, month=sep, pages={731–734} }

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