DOI: 10.1182/blood.v59.1.194.194. Human platelet surface binding of endogenous secreted factor VIII-von Willebrand factor and platelet factor 4

Human platelet surface binding of endogenous secreted factor VIII-von Willebrand factor and platelet factor 4

10.1182/blood.v59.1.194.194

Crossref journal-article

American Society of Hematology

Blood (234)

Abstract

Abstract Washed human platelets in buffers containing either 2 mM Ca++ or 4 mM EDTA were stimulated by human alpha-thrombin to induce secretion. The binding of two endogenous secreted proteins, factor-VIII-related protein (VIII-R) (von Willebrand factor) and platelet factor 4, was measured by reacting thrombin-treated and control platelets with specific antibodies to these proteins, then quantifying antibody binding with 125I-staphylococcal protein A. Both of these granule proteins were associated with the platelet membrane surface by a calcium-dependent mechanism after thrombin-induced secretion. This ability to bind endogenous secreted proteins to the plasma membrane surface may provide a mechanism by which the platelet can concentrate and organize its secreted proteins for subsequent physiologic reactions.

Bibliography

George, J., & Onofre, A. (1982). Human platelet surface binding of endogenous secreted factor VIII-von Willebrand factor and platelet factor 4. Blood, 59(1), 194â197.

Authors 2

JN George (first)
AR Onofre (additional)

References 0 Referenced 56

None

Dates

Type	When
Created	5 years, 10 months ago (Oct. 12, 2019, 10:47 p.m.)
Deposited	5 years, 9 months ago (Nov. 19, 2019, 10:20 a.m.)
Indexed	11 months, 2 weeks ago (Sept. 15, 2024, 7:10 p.m.)
Issued	43 years, 8 months ago (Jan. 1, 1982)
Published	43 years, 8 months ago (Jan. 1, 1982)
Published Print	43 years, 8 months ago (Jan. 1, 1982)

Funders 0

None

BibTeX

@article{George_1982, title={Human platelet surface binding of endogenous secreted factor VIII-von Willebrand factor and platelet factor 4}, volume={59}, ISSN={1528-0020}, url={http://dx.doi.org/10.1182/blood.v59.1.194.194}, DOI={10.1182/blood.v59.1.194.194}, number={1}, journal={Blood}, publisher={American Society of Hematology}, author={George, JN and Onofre, AR}, year={1982}, month=jan, pages={194–197} }

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