DOI: 10.1182/blood.v40.6.823.823. Hemoglobin Biosynthesis in Murine Virus-induced Leukemic Cells In Vitro: Structure and Amounts of Globin Chains Produced

Hemoglobin Biosynthesis in Murine Virus-induced Leukemic Cells In Vitro: Structure and Amounts of Globin Chains Produced

10.1182/blood.v40.6.823.823

Crossref journal-article

American Society of Hematology

Blood (234)

Abstract

Abstract Tryptic peptides characteristic of mouse hemoglobin α- and diffuse-type β-chains were isolated from dimethylsulfoxide-treated mouse leukemia virus-infected cell cultures. Quantitative estimates of in vitro hemoglobin synthesis by such cultures were obtained by two different methods: by analysis of radioactive amino acid incorporation, and by single-cell radial immunodiffusion assay. The amounts produced, approximately 4 pg/cell/17 hr, reflect a synthetic capacity that is probably sufficient for 32P labeling of hemoglobin messenger RNAs (mRNA) in quantities commensurate with structural characterization of mRNAs by radioautographic methods.

Bibliography

Boyer, S. H., Wuu, K. D., Noyes, A. N., Young, R., Scher, W., Friend, C., Preisler, H. D., & Bank, A. (1972). Hemoglobin Biosynthesis in Murine Virus-induced Leukemic Cells In Vitro: Structure and Amounts of Globin Chains Produced. Blood, 40(6), 823â835.

Authors 8

Samuel H. Boyer (first)
Kuang Dong Wuu (additional)
Andrea N. Noyes (additional)
Rondall Young (additional)
William Scher (additional)
Charlotte Friend (additional)
Harvey D. Preisler (additional)
Arthur Bank (additional)

References 0 Referenced 73

None

Dates

Type	When
Created	5 years, 10 months ago (Oct. 12, 2019, 9:45 a.m.)
Deposited	5 years, 9 months ago (Nov. 19, 2019, 2:01 p.m.)
Indexed	1 year, 7 months ago (Jan. 12, 2024, 6 p.m.)
Issued	52 years, 8 months ago (Dec. 1, 1972)
Published	52 years, 8 months ago (Dec. 1, 1972)
Published Print	52 years, 8 months ago (Dec. 1, 1972)

Funders 0

None

BibTeX

@article{Boyer_1972, title={Hemoglobin Biosynthesis in Murine Virus-induced Leukemic Cells In Vitro: Structure and Amounts of Globin Chains Produced}, volume={40}, ISSN={1528-0020}, url={http://dx.doi.org/10.1182/blood.v40.6.823.823}, DOI={10.1182/blood.v40.6.823.823}, number={6}, journal={Blood}, publisher={American Society of Hematology}, author={Boyer, Samuel H. and Wuu, Kuang Dong and Noyes, Andrea N. and Young, Rondall and Scher, William and Friend, Charlotte and Preisler, Harvey D. and Bank, Arthur}, year={1972}, month=dec, pages={823–835} }

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