DOI: 10.1182/blood.v100.3.897. Stomatin is a major lipid-raft component of platelet α granules

Stomatin is a major lipid-raft component of platelet α granules

10.1182/blood.v100.3.897

Crossref journal-article

American Society of Hematology

Blood (234)

Abstract

AbstractLipid rafts are detergent-resistant, cholesterol- and sphingolipid-rich membrane domains that are involved in important cellular processes such as signal transduction and intracellular trafficking. Stomatin, a major lipid-raft component of erythrocytes and epithelial cells, is also an abundant platelet protein. Microscopical methods and subcellular fractionation showed that stomatin is located mainly at the α-granular membrane. The lipid-raft marker proteins flotillin-1 and flotillin-2 were also present in platelets but excluded from α granules. Stomatin and the flotillins were associated with Triton X-100–insoluble lipid rafts. Whereas stomatin was partly soluble in Triton X-100, it was insoluble in the detergents Lubrol and 3-[(3-cholamidopropyl)dimethylamonio]-1-propyl sulfonate (CHAPS). Flotation experiments after CHAPS lysis of platelets revealed a distinct set of lipid-raft–associated proteins, which were identified by matrix-assisted laser desorption/ionization mass spectrometry as stomatin, flotillin-1, flotillin-2, CD36, CD9, integrin αIIbβ3, and the glucose transporter GLUT-3. Stomatin, the flotillins, and CD36 were exclusively present in this lipid-raft fraction. Activation of platelets by calcium ionophore A23187 or thrombin led to translocation of stomatin to the plasma membrane, cleavage by calpain, and specific sorting into released microvesicles. In conclusion, this study demonstrated the existence of α-granular lipid rafts and suggests an important role for stomatin in the organization and function of α granules.

Bibliography

Mairhofer, M., Steiner, M., Mosgoeller, W., Prohaska, R., & Salzer, U. (2002). Stomatin is a major lipid-raft component of platelet Î± granules. Blood, 100(3), 897â904.

Authors 5

Mario Mairhofer (first)
Marianne Steiner (additional)
Wilhelm Mosgoeller (additional)
Rainer Prohaska (additional)
Ulrich Salzer (additional)

References 54 Referenced 82

10.1016/0005-2736(91)90230-6 / Biochim Biophys Acta. / Isolation and partial characterization of the human erythrocyte band 7 integral membrane protein. by Hiebl-Dirschmied (1991)
10.1016/0167-4781(91)90047-P / Biochim Biophys Acta. / Cloning and nucleotide sequence of cDNA encoding human erythrocyte band 7 integral membrane protein. by Hiebl-Dirschmied (1991)
10.1016/S0021-9258(18)55202-9 / J Biol Chem. / Purification of band 7.2b, a 31-kDa integral phosphoprotein absent in hereditary stomatocytosis. by Wang (1991)
10.1182/blood.V79.6.1593.1593 / Blood. / Isolation of cDNA coding for an ubiquitous membrane protein deficient in high Na+, low K+ stomatocytic erythrocytes. by Stewart (1992)
10.1172/JCI110726 / J Clin Invest. / Missing band 7 membrane protein in two patients with high Na, low K erythrocytes. by Lande (1982)
10.1016/0925-4439(93)90116-I / Biochim Biophys Acta. / Stomatin: a putative cation transport regulator in the red cell membrane. by Stewart (1993)
10.1182/blood.V93.7.2404 / Blood. / Stomatocytosis is absent in “stomatin”-deficient murine red blood cells. by Zhu (1999)
10.1074/jbc.273.27.17221 / J Biol Chem. / Oligomeric nature of the integral membrane protein stomatin. by Snyers (1998)
10.1016/S0171-9335(99)80031-4 / Eur J Cell Biol. / Association of stomatin with lipid-protein complexes in the plasma membrane and the endocytic compartment. by Snyers (1999)
10.1016/0005-2736(93)90098-K / Biochim Biophys Acta. / Identification of the phosphorylation site on human erythrocyte band 7 integral membrane protein: implications for a monotopic protein structure. by Salzer (1993)
10.1016/S0968-0004(99)01467-X / Trends Biochem Sci. / The SPFH domain: implicated in regulating targeted protein turnover in stomatins and other membrane-associated proteins. by Tavernarakis (1999)
10.1182/blood.V97.4.1141 / Blood. / Stomatin, flotillin-1, and flotillin-2 are major integral proteins of erythrocyte lipid rafts. by Salzer (2001)
10.1021/bi00417a001 / Biochemistry. / Lipid sorting in epithelial cells. by Simons (1988)
10.1016/0092-8674(90)90357-K / Cell. / Polarized sorting in epithelia. by Simons (1990)
10.1038/42408 / Nature. / Functional rafts in cell membranes. by Simons (1997)
10.1038/35036052 / Nat Rev Mol Cell Biol. / Lipid rafts and signal transduction. by Simons (2000)
10.1074/jbc.R000005200 / J Biol Chem. / Structure and function of sphingolipid- and cholesterol-rich membrane rafts. by Brown (2000)
10.1146/annurev.cellbio.14.1.111 / Annu Rev Cell Dev Biol. / Functions of lipid rafts in biological membranes. by Brown (1998)
10.1126/science.7660128 / Science. / Separation of caveolae from associated microdomains of GPI-anchored proteins. by Schnitzer (1995)
10.1038/35023524 / Nat Cell Biol. / Retention of prominin in microvilli reveals distinct cholesterol-based lipid micro-domains in the apical plasma membrane. by Roper (2000)
10.1093/emboj/18.24.6917 / EMBO J. / Functionally different GPI proteins are organized in different domains on the neuronal surface. by Madore (1999)
10.1091/mbc.11.8.2775 / Mol Biol Cell. / The recycling endosome of Madin-Darby canine kidney cells is a mildly acidic compartment rich in raft components. by Gagescu (2000)
10.1091/mbc.12.6.1819 / Mol Biol Cell. / Sphingomyelin-enriched microdomains at the golgi complex. by Gkantiragas (2001)
10.1074/jbc.M101113200 / J Biol Chem. / Flotillin-1-enriched lipid raft domains accumulate on maturing phagosomes. by Dermine (2001)
10.1074/jbc.272.21.13793 / J Biol Chem. / Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins. by Bickel (1997)
10.1002/(SICI)1097-4695(199812)37:4<502::AID-NEU2>3.0.CO;2-S / J Neurobiol. / Identification of reggie-1 and reggie-2 as plasma membrane-associated proteins which cocluster with activated GPI-anchored cell adhesion molecules in non-caveolar micropatches in neurons. by Lang (1998)
10.1042/bj3190067 / Biochem J. / Biochemical isolation of a membrane microdomain from resting platelets highly enriched in the plasma membrane glycoprotein CD36. by Dorahy (1996)
10.1042/bj3330373 / Biochem J. / Active Lyn protein tyrosine kinase is selectively enriched within membrane microdomains of resting platelets. by Dorahy (1998)
10.1182/blood.V82.10.3034.3034 / Blood. / Ultrastructural demonstration of CD36 in the α-granule membrane of human platelets and megakaryocytes. by Berger (1993)
10.1182/blood.V87.4.1385.bloodjournal8741385 / Blood. / α-granule membrane mirrors the platelet plasma membrane and contains the glycoproteins Ib, IX, and V. by Berger (1996)
10.1172/JCI114300 / J Clin Invest. / Uptake of plasma fibrinogen into the α granules of human megakaryocytes and platelets. by Harrison (1989)
10.1182/blood.V82.1.135.bloodjournal821135 / Blood. / Endocytosis of fibrinogen into megakaryocyte and platelet α-granules is mediated by αIIb β3 (glycoprotein IIb-IIIa). by Handagama (1993)
{'key': '2020021300153051300_B33', 'first-page': '2384', 'volume': '89', 'author': 'Stenberg', 'year': '1997', 'journal-title': 'The Src family kinases'} / The Src family kinases by Stenberg (1997)
10.1242/jcs.109.2.409 / J Cell Sci. / Nuclear architecture and ultrastructural distribution of poly(ADP-ribosyl) transferase, a multifunctional enzyme. by Mosgoeller (1996)
10.1182/blood.V94.4.1300 / Blood. / Synthesis and ultrastructural localization of protein C inhibitor in human platelets and megakaryocytes. by Prendes (1999)
10.1177/41.10.7504007 / J Histochem Cytochem. / Distribution of DNA in human Sertoli cell nucleoli. by Mosgoller (1993)
10.1182/blood.V94.11.3791 / Blood. / Activated platelets release two types of membrane vesicles: microvesicles by surface shedding and exosomes derived from exocytosis of multivesicular bodies and α-granules. by Heijnen (1999)
10.1016/S0006-291X(89)80065-8 / Biochem Biophys Res Commun. / Inhibition of calpain in intact platelets by the thiol protease inhibitor E-64d. by McGowan (1989)
10.1016/S0006-291X(88)81355-X / Biochem Biophys Res Commun. / Synthesis of a new cell penetrating calpain inhibitor (calpeptin). by Tsujinaka (1988)
10.1016/0076-6879(92)15049-I / Methods Enzymol. / Homogenization by nitrogen cavitation technique applied to platelet subcellular fractionation. by Broekman (1992)
10.1083/jcb.151.1.143 / J Cell Biol. / Assembly of myelin by association of proteolipid protein with cholesterol- and galactosylceramide-rich membrane domains. by Simons (2000)
10.1016/S0021-9258(18)98837-X / J Biol Chem. / Evidence that agonist-induced activation of calpain causes the shedding of procoagulant-containing microvesicles from the membrane of aggregating platelets. by Fox (1991)
10.1074/jbc.270.45.27259 / J Biol Chem. / Dystrophin-related protein in the platelet membrane skeleton. Integrin-induced change in detergent-insolubility and cleavage by calpain in aggregating platelets. by Earnest (1995)
10.1016/S0021-9258(18)31431-5 / J Biol Chem. / pp60src is an endogenous substrate for calpain in human blood platelets. by Oda (1993)
10.1093/oso/9780199635382.003.0003 / Platelets. A Practical Approach. / Preparation and uses of semi-permeabilized platelets. by Scrutton (1996)
10.1016/S0021-9258(19)47235-9 / J Biol Chem. / Inhibition of calpain by a synthetic oligopeptide corresponding to an exon of the human calpastatin gene. by Maki (1989)
{'key': '2020021300153051300_B47', 'first-page': '3334', 'article-title': 'Molecular mechanisms of platelet exocytosis: insights into the “secrete” life of thrombocytes.', 'volume': '96', 'author': 'Reed', 'year': '2000', 'journal-title': 'Blood.'} / Blood. / Molecular mechanisms of platelet exocytosis: insights into the “secrete” life of thrombocytes. by Reed (2000)
10.1073/pnas.091090798 / Proc Natl Acad Sci U S A. / Selective binding of perfringolysin O derivative to cholesterol-rich membrane microdomains (rafts). by Waheed (2001)
10.1182/blood.V97.6.1689 / Blood. / CD36 associates with CD9 and integrins on human blood platelets. by Miao (2001)
10.1083/jcb.138.2.323 / J Cell Biol. / Thrombin stimulates glucose transport in human platelets via the translocation of the glucose transporter GLUT-3 from α-granules to the cell surface. by Heijnen (1997)
10.1042/bj3280511 / Biochem J. / Thrombin-induced translocation of GLUT3 glucose transporters in human platelets. by Sorbara (1997)
10.1006/abbi.1999.1489 / Arch Biochem Biophys. / Association of stomatin (band 7.2b) with Glut1 glucose transporter. by Zhang (1999)
10.1152/ajpcell.2001.280.5.C1277 / Am J Physiol Cell Physiol. / Overexpression of stomatin depresses GLUT-1 glucose transporter activity. by Zhang (2001)
10.1182/blood.V94.5.1683 / Blood. / Calpain functions in a caspase-independent manner to promote apoptosis-like events during platelet activation. by Wolf (1999)

Dates

Type	When
Created	22 years, 10 months ago (Oct. 11, 2002, 2:33 p.m.)
Deposited	1 year, 7 months ago (Jan. 6, 2024, 1:46 p.m.)
Indexed	3 weeks, 3 days ago (Aug. 12, 2025, 5:30 p.m.)
Issued	23 years, 1 month ago (Aug. 1, 2002)
Published	23 years, 1 month ago (Aug. 1, 2002)
Published Print	23 years, 1 month ago (Aug. 1, 2002)

Funders 0

None

BibTeX

@article{Mairhofer_2002, title={Stomatin is a major lipid-raft component of platelet α granules}, volume={100}, ISSN={0006-4971}, url={http://dx.doi.org/10.1182/blood.v100.3.897}, DOI={10.1182/blood.v100.3.897}, number={3}, journal={Blood}, publisher={American Society of Hematology}, author={Mairhofer, Mario and Steiner, Marianne and Mosgoeller, Wilhelm and Prohaska, Rainer and Salzer, Ulrich}, year={2002}, month=aug, pages={897–904} }

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      "journal-title": "Biochim Biophys Acta."
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      "volume": "1090",
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      "first-page": "17826",
      "DOI": "10.1016/S0021-9258(18)55202-9",
      "article-title": "Purification of band 7.2b, a 31-kDa integral phosphoprotein absent in hereditary stomatocytosis.",
      "volume": "266",
      "author": "Wang",
      "year": "1991",
      "journal-title": "J Biol Chem."
    },
    {
      "key": "2020021300153051300_B4",
      "doi-asserted-by": "crossref",
      "first-page": "1593",
      "DOI": "10.1182/blood.V79.6.1593.1593",
      "article-title": "Isolation of cDNA coding for an ubiquitous membrane protein deficient in high Na+, low K+ stomatocytic erythrocytes.",
      "volume": "79",
      "author": "Stewart",
      "year": "1992",
      "journal-title": "Blood."
    },
    {
      "key": "2020021300153051300_B5",
      "doi-asserted-by": "crossref",
      "first-page": "1273",
      "DOI": "10.1172/JCI110726",
      "article-title": "Missing band 7 membrane protein in two patients with high Na, low K erythrocytes.",
      "volume": "70",
      "author": "Lande",
      "year": "1982",
      "journal-title": "J Clin Invest."
    },
    {
      "key": "2020021300153051300_B6",
      "doi-asserted-by": "crossref",
      "first-page": "15",
      "DOI": "10.1016/0925-4439(93)90116-I",
      "article-title": "Stomatin: a putative cation transport regulator in the red cell membrane.",
      "volume": "1225",
      "author": "Stewart",
      "year": "1993",
      "journal-title": "Biochim Biophys Acta."
    },
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      "first-page": "2404",
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      "article-title": "Stomatocytosis is absent in \u201cstomatin\u201d-deficient murine red blood cells.",
      "volume": "93",
      "author": "Zhu",
      "year": "1999",
      "journal-title": "Blood."
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      "first-page": "17221",
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      "volume": "273",
      "author": "Snyers",
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    {
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      "volume": "78",
      "author": "Snyers",
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      "journal-title": "Eur J Cell Biol."
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      "volume": "1151",
      "author": "Salzer",
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      "volume": "24",
      "author": "Tavernarakis",
      "year": "1999",
      "journal-title": "Trends Biochem Sci."
    },
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      "volume": "97",
      "author": "Salzer",
      "year": "2001",
      "journal-title": "Blood."
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      "volume": "27",
      "author": "Simons",
      "year": "1988",
      "journal-title": "Biochemistry."
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      "article-title": "Polarized sorting in epithelia.",
      "volume": "62",
      "author": "Simons",
      "year": "1990",
      "journal-title": "Cell."
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      "key": "2020021300153051300_B15",
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      "first-page": "569",
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      "author": "Simons",
      "year": "1997",
      "journal-title": "Nature."
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      "key": "2020021300153051300_B16",
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      "volume": "275",
      "author": "Brown",
      "year": "2000",
      "journal-title": "J Biol Chem."
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      "volume": "14",
      "author": "Brown",
      "year": "1998",
      "journal-title": "Annu Rev Cell Dev Biol."
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      "first-page": "1435",
      "DOI": "10.1126/science.7660128",
      "article-title": "Separation of caveolae from associated microdomains of GPI-anchored proteins.",
      "volume": "269",
      "author": "Schnitzer",
      "year": "1995",
      "journal-title": "Science."
    },
    {
      "key": "2020021300153051300_B20",
      "doi-asserted-by": "crossref",
      "first-page": "582",
      "DOI": "10.1038/35023524",
      "article-title": "Retention of prominin in microvilli reveals distinct cholesterol-based lipid micro-domains in the apical plasma membrane.",
      "volume": "2",
      "author": "Roper",
      "year": "2000",
      "journal-title": "Nat Cell Biol."
    },
    {
      "key": "2020021300153051300_B21",
      "doi-asserted-by": "crossref",
      "first-page": "6917",
      "DOI": "10.1093/emboj/18.24.6917",
      "article-title": "Functionally different GPI proteins are organized in different domains on the neuronal surface.",
      "volume": "18",
      "author": "Madore",
      "year": "1999",
      "journal-title": "EMBO J."
    },
    {
      "key": "2020021300153051300_B22",
      "doi-asserted-by": "crossref",
      "first-page": "2775",
      "DOI": "10.1091/mbc.11.8.2775",
      "article-title": "The recycling endosome of Madin-Darby canine kidney cells is a mildly acidic compartment rich in raft components.",
      "volume": "11",
      "author": "Gagescu",
      "year": "2000",
      "journal-title": "Mol Biol Cell."
    },
    {
      "key": "2020021300153051300_B23",
      "doi-asserted-by": "crossref",
      "first-page": "1819",
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      "article-title": "Sphingomyelin-enriched microdomains at the golgi complex.",
      "volume": "12",
      "author": "Gkantiragas",
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      "journal-title": "Mol Biol Cell."
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      "article-title": "Flotillin-1-enriched lipid raft domains accumulate on maturing phagosomes.",
      "volume": "276",
      "author": "Dermine",
      "year": "2001",
      "journal-title": "J Biol Chem."
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      "first-page": "13793",
      "DOI": "10.1074/jbc.272.21.13793",
      "article-title": "Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins.",
      "volume": "272",
      "author": "Bickel",
      "year": "1997",
      "journal-title": "J Biol Chem."
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      "key": "2020021300153051300_B26",
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      "first-page": "502",
      "DOI": "10.1002/(SICI)1097-4695(199812)37:4<502::AID-NEU2>3.0.CO;2-S",
      "article-title": "Identification of reggie-1 and reggie-2 as plasma membrane-associated proteins which cocluster with activated GPI-anchored cell adhesion molecules in non-caveolar micropatches in neurons.",
      "volume": "37",
      "author": "Lang",
      "year": "1998",
      "journal-title": "J Neurobiol."
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      "first-page": "67",
      "DOI": "10.1042/bj3190067",
      "article-title": "Biochemical isolation of a membrane microdomain from resting platelets highly enriched in the plasma membrane glycoprotein CD36.",
      "volume": "319",
      "author": "Dorahy",
      "year": "1996",
      "journal-title": "Biochem J."
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      "doi-asserted-by": "crossref",
      "first-page": "373",
      "DOI": "10.1042/bj3330373",
      "article-title": "Active Lyn protein tyrosine kinase is selectively enriched within membrane microdomains of resting platelets.",
      "volume": "333",
      "author": "Dorahy",
      "year": "1998",
      "journal-title": "Biochem J."
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      "first-page": "3034",
      "DOI": "10.1182/blood.V82.10.3034.3034",
      "article-title": "Ultrastructural demonstration of CD36 in the \u03b1-granule membrane of human platelets and megakaryocytes.",
      "volume": "82",
      "author": "Berger",
      "year": "1993",
      "journal-title": "Blood."
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      "first-page": "1385",
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      "year": "1996",
      "journal-title": "Blood."
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      "first-page": "1320",
      "DOI": "10.1172/JCI114300",
      "article-title": "Uptake of plasma fibrinogen into the \u03b1 granules of human megakaryocytes and platelets.",
      "volume": "84",
      "author": "Harrison",
      "year": "1989",
      "journal-title": "J Clin Invest."
    },
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      "first-page": "135",
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      "volume": "82",
      "author": "Handagama",
      "year": "1993",
      "journal-title": "Blood."
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      "key": "2020021300153051300_B33",
      "first-page": "2384",
      "volume": "89",
      "author": "Stenberg",
      "year": "1997",
      "journal-title": "The Src family kinases"
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      "first-page": "409",
      "DOI": "10.1242/jcs.109.2.409",
      "article-title": "Nuclear architecture and ultrastructural distribution of poly(ADP-ribosyl) transferase, a multifunctional enzyme.",
      "volume": "109",
      "author": "Mosgoeller",
      "year": "1996",
      "journal-title": "J Cell Sci."
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      "volume": "94",
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      "article-title": "Distribution of DNA in human Sertoli cell nucleoli.",
      "volume": "41",
      "author": "Mosgoller",
      "year": "1993",
      "journal-title": "J Histochem Cytochem."
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      "author": "Heijnen",
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      "article-title": "Inhibition of calpain in intact platelets by the thiol protease inhibitor E-64d.",
      "volume": "158",
      "author": "McGowan",
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      "journal-title": "Biochem Biophys Res Commun."
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      "article-title": "Synthesis of a new cell penetrating calpain inhibitor (calpeptin).",
      "volume": "153",
      "author": "Tsujinaka",
      "year": "1988",
      "journal-title": "Biochem Biophys Res Commun."
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      "first-page": "21",
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      "volume": "215",
      "author": "Broekman",
      "year": "1992",
      "journal-title": "Methods Enzymol."
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      "first-page": "143",
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      "volume": "151",
      "author": "Simons",
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      "journal-title": "J Cell Biol."
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      "author": "Fox",
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      "journal-title": "J Biol Chem."
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      "volume": "270",
      "author": "Earnest",
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      "first-page": "47",
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      "volume": "167",
      "author": "Scrutton",
      "year": "1996",
      "journal-title": "Platelets. A Practical Approach."
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      "volume": "264",
      "author": "Maki",
      "year": "1989",
      "journal-title": "J Biol Chem."
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      "first-page": "3334",
      "article-title": "Molecular mechanisms of platelet exocytosis: insights into the \u201csecrete\u201d life of thrombocytes.",
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      "volume": "98",
      "author": "Waheed",
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      "journal-title": "Proc Natl Acad Sci U S A."
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