DOI: 10.1182/blood-2006-06-032516. Evidence for the multimeric structure of ferroportin

Evidence for the multimeric structure of ferroportin

10.1182/blood-2006-06-032516

Crossref journal-article

American Society of Hematology

Blood (234)

Abstract

Abstract Ferroportin (Fpn) (IREG1, SLC40A1, MTP1) is an iron transporter, and mutations in Fpn result in a genetically dominant form of iron overload disease. Previously, we demonstrated that Fpn is a multimer and that mutations in Fpn are dominant negative. Other studies have suggested that Fpn is not a multimer and that overexpression or epitope tags might affect the localization, topology, or multimerization of Fpn. We generated wild-type Fpn with 3 different epitopes, GFP, FLAG, and c-myc, and expressed these constructs in cultured cells. Co-expression of any 2 different epitope-tagged proteins in the same cell resulted in their quantitative coimmunoprecipitation. Treatment of Fpn-GFP/Fpn-FLAG–expressing cells with crosslinking reagents resulted in the crosslinking of Fpn-GFP and Fpn-FLAG. Western analysis of rat glioma C6 cells or mouse bone marrow macrophages exposed to crosslinking reagents showed that endogenous Fpn is a dimer. These results support the hypothesis that the dominant inheritance of Fpn–iron overload disease is due to the dominant-negative effects of mutant Fpn proteins.

Bibliography

De Domenico, I., Ward, D. M., Musci, G., & Kaplan, J. (2006). Evidence for the multimeric structure of ferroportin. Blood, 109(5), 2205â2209.

Authors 4

Ivana De Domenico (first)
Diane McVey Ward (additional)
Giovanni Musci (additional)
Jerry Kaplan (additional)

References 20 Referenced 53

Dates

Type	When
Created	18 years, 9 months ago (Nov. 2, 2006, 3:26 a.m.)
Deposited	5 years, 9 months ago (Nov. 17, 2019, 2:33 p.m.)
Indexed	4 months, 1 week ago (April 22, 2025, 4:09 a.m.)
Issued	18 years, 10 months ago (Oct. 31, 2006)
Published	18 years, 10 months ago (Oct. 31, 2006)
Published Online	18 years, 10 months ago (Oct. 31, 2006)
Published Print	18 years, 5 months ago (March 1, 2007)

Funders 0

None

BibTeX

@article{De_Domenico_2006, title={Evidence for the multimeric structure of ferroportin}, volume={109}, ISSN={1528-0020}, url={http://dx.doi.org/10.1182/blood-2006-06-032516}, DOI={10.1182/blood-2006-06-032516}, number={5}, journal={Blood}, publisher={American Society of Hematology}, author={De Domenico, Ivana and Ward, Diane McVey and Musci, Giovanni and Kaplan, Jerry}, year={2006}, month=oct, pages={2205–2209} }

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