DOI: 10.1182/blood-2006-01-012369. MicroRNAs modulate the angiogenic properties of HUVECs

MicroRNAs modulate the angiogenic properties of HUVECs

10.1182/blood-2006-01-012369

Crossref journal-article

American Society of Hematology

Blood (234)

Abstract

AbstractMicroRNAs (miRNAs) have recently come into focus as key posttranscriptional modulators of gene expression. In this work, we addressed whether in vitro angiogenesis is an miRNA-regulated process. We performed large-scale analysis of miRNA expression in human umbilical vein endothelial cells (HUVECs) and found that 15 highly expressed miRNAs have the receptors of angiogenic factors as putative targets. In particular, we demonstrated that miR-221 and miR-222 affect c-Kit expression and, as a consequence, the angiogenic properties of its ligand stem cell factor. Interaction between miR-222 and c-Kit is likely to be part of a complex circuit that controls the ability of endothelial cells to form new capillaries.

Bibliography

Poliseno, L., Tuccoli, A., Mariani, L., Evangelista, M., Citti, L., Woods, K., Mercatanti, A., Hammond, S., & Rainaldi, G. (2006). MicroRNAs modulate the angiogenic properties of HUVECs. Blood, 108(9), 3068â3071.

Authors 9

Laura Poliseno (first)
Andrea Tuccoli (additional)
Laura Mariani (additional)
Monica Evangelista (additional)
Lorenzo Citti (additional)
Keith Woods (additional)
Alberto Mercatanti (additional)
Scott Hammond (additional)
Giuseppe Rainaldi (additional)

References 25 Referenced 606

Dates

Type	When
Created	19 years, 1 month ago (July 18, 2006, 8:49 p.m.)
Deposited	5 years, 4 months ago (April 16, 2020, 11:37 p.m.)
Indexed	25 minutes ago (Sept. 7, 2025, 9:37 a.m.)
Issued	18 years, 10 months ago (Nov. 1, 2006)
Published	18 years, 10 months ago (Nov. 1, 2006)
Published Print	18 years, 10 months ago (Nov. 1, 2006)

Funders 0

None

BibTeX

@article{Poliseno_2006, title={MicroRNAs modulate the angiogenic properties of HUVECs}, volume={108}, ISSN={1528-0020}, url={http://dx.doi.org/10.1182/blood-2006-01-012369}, DOI={10.1182/blood-2006-01-012369}, number={9}, journal={Blood}, publisher={American Society of Hematology}, author={Poliseno, Laura and Tuccoli, Andrea and Mariani, Laura and Evangelista, Monica and Citti, Lorenzo and Woods, Keith and Mercatanti, Alberto and Hammond, Scott and Rainaldi, Giuseppe}, year={2006}, month=nov, pages={3068–3071} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        9,
        7
      ]
    ],
    "date-time": "2025-09-07T13:37:19Z",
    "timestamp": 1757252239049
  },
  "reference-count": 25,
  "publisher": "American Society of Hematology",
  "issue": "9",
  "content-domain": {
    "domain": [
      "ashpublications.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2006,
        11,
        1
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>MicroRNAs (miRNAs) have recently come into focus as key posttranscriptional modulators of gene expression. In this work, we addressed whether in vitro angiogenesis is an miRNA-regulated process. We performed large-scale analysis of miRNA expression in human umbilical vein endothelial cells (HUVECs) and found that 15 highly expressed miRNAs have the receptors of angiogenic factors as putative targets. In particular, we demonstrated that miR-221 and miR-222 affect c-Kit expression and, as a consequence, the angiogenic properties of its ligand stem cell factor. Interaction between miR-222 and c-Kit is likely to be part of a complex circuit that controls the ability of endothelial cells to form new capillaries.</jats:p>",
  "DOI": "10.1182/blood-2006-01-012369",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        7,
        19
      ]
    ],
    "date-time": "2006-07-19T00:49:11Z",
    "timestamp": 1153270151000
  },
  "page": "3068-3071",
  "update-policy": "http://dx.doi.org/10.1182/blood.2019cm0000",
  "source": "Crossref",
  "is-referenced-by-count": 606,
  "title": "MicroRNAs modulate the angiogenic properties of HUVECs",
  "prefix": "10.1182",
  "volume": "108",
  "author": [
    {
      "given": "Laura",
      "family": "Poliseno",
      "sequence": "first",
      "affiliation": [
        {
          "name": "From the Laboratory of Gene and Molecular Therapy, Institute of Clinical Physiology, CNR, Pisa, Italy; and the Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC."
        }
      ]
    },
    {
      "given": "Andrea",
      "family": "Tuccoli",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "From the Laboratory of Gene and Molecular Therapy, Institute of Clinical Physiology, CNR, Pisa, Italy; and the Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC."
        }
      ]
    },
    {
      "given": "Laura",
      "family": "Mariani",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "From the Laboratory of Gene and Molecular Therapy, Institute of Clinical Physiology, CNR, Pisa, Italy; and the Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC."
        }
      ]
    },
    {
      "given": "Monica",
      "family": "Evangelista",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "From the Laboratory of Gene and Molecular Therapy, Institute of Clinical Physiology, CNR, Pisa, Italy; and the Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC."
        }
      ]
    },
    {
      "given": "Lorenzo",
      "family": "Citti",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "From the Laboratory of Gene and Molecular Therapy, Institute of Clinical Physiology, CNR, Pisa, Italy; and the Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC."
        }
      ]
    },
    {
      "given": "Keith",
      "family": "Woods",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "From the Laboratory of Gene and Molecular Therapy, Institute of Clinical Physiology, CNR, Pisa, Italy; and the Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC."
        }
      ]
    },
    {
      "given": "Alberto",
      "family": "Mercatanti",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "From the Laboratory of Gene and Molecular Therapy, Institute of Clinical Physiology, CNR, Pisa, Italy; and the Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC."
        }
      ]
    },
    {
      "given": "Scott",
      "family": "Hammond",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "From the Laboratory of Gene and Molecular Therapy, Institute of Clinical Physiology, CNR, Pisa, Italy; and the Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC."
        }
      ]
    },
    {
      "given": "Giuseppe",
      "family": "Rainaldi",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "From the Laboratory of Gene and Molecular Therapy, Institute of Clinical Physiology, CNR, Pisa, Italy; and the Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC."
        }
      ]
    }
  ],
  "member": "234",
  "reference": [
    {
      "key": "2019111711193745100_REF1",
      "doi-asserted-by": "crossref",
      "unstructured": "Carmeliet P. Angiogenesis in health and disease. Nat Med. 2003;9: 653-660.",
      "DOI": "10.1038/nm0603-653"
    },
    {
      "key": "2019111711193745100_REF2",
      "doi-asserted-by": "crossref",
      "unstructured": "Kim VN. MicroRNA biogenesis: coordinated cropping and dicing. Nat Rev Mol Cell Biol. 2005;6: 376-385.",
      "DOI": "10.1038/nrm1644"
    },
    {
      "key": "2019111711193745100_REF3",
      "doi-asserted-by": "crossref",
      "unstructured": "Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004;116: 281-297.",
      "DOI": "10.1016/S0092-8674(04)00045-5"
    },
    {
      "key": "2019111711193745100_REF4",
      "doi-asserted-by": "crossref",
      "unstructured": "Pillai RS, Bhattacharyya SN, Artus CG, et al. Inhibition of translational initiation by Let-7 MicroRNA in human cells. Science. 2005;309: 1573-1576.",
      "DOI": "10.1126/science.1115079"
    },
    {
      "key": "2019111711193745100_REF5",
      "doi-asserted-by": "crossref",
      "unstructured": "Yang WJ, Yang DD, Na S, Sandusky GE, Zhang Q, Zhao G. Dicer is required for embryonic angiogenesis during mouse development. J Biol Chem. 2005;280: 9330-9335.",
      "DOI": "10.1074/jbc.M413394200"
    },
    {
      "key": "2019111711193745100_REF6",
      "doi-asserted-by": "crossref",
      "unstructured": "Chen CZ, Li L, Lodish HF, Bartel DP. MicroRNAs modulate hematopoietic lineage differentiation. Science. 2004;303: 83-86.",
      "DOI": "10.1126/science.1091903"
    },
    {
      "key": "2019111711193745100_REF7",
      "doi-asserted-by": "crossref",
      "unstructured": "Zhao Y, Samal E, Srivastava D. Serum response factor regulates a muscle-specific microRNA that targets Hand2 during cardiogenesis. Nature. 2005;436: 214-220.",
      "DOI": "10.1038/nature03817"
    },
    {
      "key": "2019111711193745100_REF8",
      "doi-asserted-by": "crossref",
      "unstructured": "Fazi F, Rosa A, Fatica A, et al. A minicircuitry comprised of microRNA-223 and transcription factors NFI-A and C/EBP\u03b1 regulates human granulopoiesis. Cell. 2005;123: 819-831.",
      "DOI": "10.1016/j.cell.2005.09.023"
    },
    {
      "key": "2019111711193745100_REF9",
      "doi-asserted-by": "crossref",
      "unstructured": "Thomson JM, Parker J, Perou CM, Hammond SM. A custom microarray platform for analysis of microRNA gene expression. Nat Methods. 2004;1: 47-53.",
      "DOI": "10.1038/nmeth704"
    },
    {
      "key": "2019111711193745100_REF10",
      "doi-asserted-by": "crossref",
      "unstructured": "Matsui J, Wakabayashi T, Asada M, Yoshimatsu K, Okada M. Stem cell factor/c-kit signaling promotes the survival, migration, and capillary tube formation of human umbilical vein endothelial cells. J Biol Chem. 2004;279: 18600-18607.",
      "DOI": "10.1074/jbc.M311643200"
    },
    {
      "key": "2019111711193745100_REF11",
      "doi-asserted-by": "crossref",
      "unstructured": "Poliseno L, Evangelista M, Ricci F, Bonotti A, Nannipieri M, Rainaldi G. Identification of active siRNAs against IGF-IR of porcine coronary smooth muscle cells in a heterologous cell line. Int J Mol Med. 2005;15: 713-718.",
      "DOI": "10.3892/ijmm.15.4.713"
    },
    {
      "key": "2019111711193745100_REF12",
      "doi-asserted-by": "crossref",
      "unstructured": "Neufeld G, Cohen T, Gengrinovitch S, Poltorak Z. Vascular endothelial growth factor (VEGF) and its receptors. FASEB J. 1999;13: 9-22.",
      "DOI": "10.1096/fasebj.13.1.9"
    },
    {
      "key": "2019111711193745100_REF13",
      "doi-asserted-by": "crossref",
      "unstructured": "Broudy VC, Kovach NL, Bennett LG, Lin N, Jacobsen FW, Kidd PG. Human umbilical vein endothelial cells display high-affinity c-kit receptors and produce a soluble form of the c-kit receptor. Blood. 1994;83: 2145-2152.",
      "DOI": "10.1182/blood.V83.8.2145.2145"
    },
    {
      "key": "2019111711193745100_REF14",
      "doi-asserted-by": "crossref",
      "unstructured": "Lewis BP, Shih IH, Jones-Rhoades MW, Bartel DP, Burge CB. Prediction of mammalian microRNA targets. Cell. 2003;115: 787-798.",
      "DOI": "10.1016/S0092-8674(03)01018-3"
    },
    {
      "key": "2019111711193745100_REF15",
      "doi-asserted-by": "crossref",
      "unstructured": "Lewis BP, Burge CB, Bartel DP. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell. 2005;120: 15-20.",
      "DOI": "10.1016/j.cell.2004.12.035"
    },
    {
      "key": "2019111711193745100_REF16",
      "doi-asserted-by": "crossref",
      "unstructured": "Krek A, Grun D, Poy MN, et al. Combinatorial microRNA target predictions. Nat Genet. 2005;37: 495-500.",
      "DOI": "10.1038/ng1536"
    },
    {
      "key": "2019111711193745100_REF17",
      "doi-asserted-by": "crossref",
      "unstructured": "John B, Enright AJ, Aravin A, Tuschl T, Sander C, Marks DS. Human MicroRNA targets. PLoS Biol. 2004;2: e363.",
      "DOI": "10.1371/journal.pbio.0020363"
    },
    {
      "key": "2019111711193745100_REF18",
      "doi-asserted-by": "crossref",
      "unstructured": "Calin GA, Sevignani C, Dumitru CD, et al. Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers. Proc Natl Acad Sci U S A. 2004;101: 2999-3004.",
      "DOI": "10.1073/pnas.0307323101"
    },
    {
      "key": "2019111711193745100_REF19",
      "doi-asserted-by": "crossref",
      "unstructured": "Altuvia Y, Landgraf P, Lithwick G, et al. Clustering and conservation patterns of human microRNAs. Nucleic Acids Res. 2005;33: 2697-2706.",
      "DOI": "10.1093/nar/gki567"
    },
    {
      "key": "2019111711193745100_REF20",
      "doi-asserted-by": "crossref",
      "unstructured": "Baskerville S, Bartel DP. Microarray profiling of microRNAs reveals frequent coexpression with neighboring miRNAs and host genes. RNA. 2005;11: 241-247.",
      "DOI": "10.1261/rna.7240905"
    },
    {
      "key": "2019111711193745100_REF21",
      "doi-asserted-by": "crossref",
      "unstructured": "Poliseno L, Evangelista M, Mercatanti A, Mariani L, Citti L, Rainaldi G. The energy profiling of short interfering RNAs is highly predictive of their activity. Oligonucleotides. 2004;14: 227-232.",
      "DOI": "10.1089/oli.2004.14.227"
    },
    {
      "key": "2019111711193745100_REF22",
      "doi-asserted-by": "crossref",
      "unstructured": "Felli N, Fontana L, Pelosi E, et al. MicroRNAs 221 and 222 inhibit normal erythropoiesis and erythroleukemic cell growth via kit receptor down-modulation. Proc Natl Acad Sci U S A. 2005;102: 18081-18086.",
      "DOI": "10.1073/pnas.0506216102"
    },
    {
      "key": "2019111711193745100_",
      "doi-asserted-by": "crossref",
      "unstructured": "Basta G, Lazzerini G, Massaro M, et al. Advanced glycation end products activate endothelium through signal-transduction receptor RAGE: a mechanism for amplification of inflammatory responses. Circulation. 2002;105: 816-822.",
      "DOI": "10.1161/hc0702.104183"
    },
    {
      "key": "2019111711193745100_",
      "doi-asserted-by": "crossref",
      "unstructured": "Yarden Y, Kuang WJ, Yang-Feng T, et al. Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO J. 1987;6: 3341-3351.",
      "DOI": "10.1002/j.1460-2075.1987.tb02655.x"
    },
    {
      "key": "2019111711193745100_",
      "doi-asserted-by": "crossref",
      "unstructured": "Sun L, Lee J, Fine HA. Neuronally expressed stem cell factor induces neural stem cell migration to areas of brain injury. J Clin Invest. 2004;113: 1364-1374.",
      "DOI": "10.1172/JCI200420001"
    }
  ],
  "container-title": "Blood",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "http://ashpublications.org/blood/article-pdf/108/9/3068/1284635/zh802106003068.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "http://ashpublications.org/blood/article-pdf/108/9/3068/1284635/zh802106003068.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2020,
        4,
        17
      ]
    ],
    "date-time": "2020-04-17T03:37:54Z",
    "timestamp": 1587094674000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://ashpublications.org/blood/article/108/9/3068/22583/MicroRNAs-modulate-the-angiogenic-properties-of"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2006,
        11,
        1
      ]
    ]
  },
  "references-count": 25,
  "journal-issue": {
    "issue": "9",
    "published-print": {
      "date-parts": [
        [
          2006,
          11,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1182/blood-2006-01-012369",
  "relation": {},
  "ISSN": [
    "0006-4971",
    "1528-0020"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        2006,
        11,
        1
      ]
    ]
  }
}