DOI: 10.1182/blood-2002-03-0898. Interaction of human hematopoietic stem cells with bacterial pathogens

Interaction of human hematopoietic stem cells with bacterial pathogens

10.1182/blood-2002-03-0898

Crossref journal-article

American Society of Hematology

Blood (234)

Abstract

Primitive hematopoietic stem cells (HSCs) in the bone marrow are rare pluripotent cells with the capacity to give rise to all lineages of blood cells. During commitment, progenitor cells are composed mainly of cells with the potential for differentiation into 1 or 2 lineages. This commitment involves the acquisition of specific growth factor receptors and the loss of others. Viral and bacterial infections may lead to profound disturbance of hematopoiesis, which is possibly due to different susceptibility of HSCs to infectious agents. Here, we show that quiescent human HSCs are fully resistant to infection by the intracellular bacteria, Listeria monocytogenes andSalmonella enterica serovariationtyphimurium, and the extracellular pathogen Yersinia enterocolitica. During myeloid/monocytic differentiation induced by incubation with stem cell factor, thrombopoietin, and flt-3 ligand, partially differentiated HSCs emerge, which readily take up these pathogens and also latex beads by macropinocytosis. After further monocytic differentiation, bacterial uptake by macropinocytosis still occurs but internalization of the pathogens is now mainly achieved by receptor-mediated phagocytosis. These results suggest that in the case of HSCs uptake mechanisms for bacteria develop sequentially.

Bibliography

Kolb-MaÌurer, A., Wilhelm, M., Weissinger, F., BroÌcker, E.-B., & Goebel, W. (2002). Interaction of human hematopoietic stem cells with bacterial pathogens. Blood, 100(10), 3703â3709.

Authors 5

Annette Kolb-Mäurer (first)
Martin Wilhelm (additional)
Florian Weissinger (additional)
Eva-Bettina Bröcker (additional)
Werner Goebel (additional)

References 26 Referenced 55

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10.1128/IAI.68.6.3680-3688.2000 / Infect Immun. / Listeria monocytogenes-infected human dendritic cells: uptake and host-cell response. by Kolb-Mäurer (2000)
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10.1128/IAI.63.9.3665-3673.1995 / Infect Immun. / Interaction of Listeria monocytogenes with mouse dendritic cells. by Guzman (1995)
10.1128/IAI.65.10.4075-4081.1997 / Infect Immun. / The mechanism of cell death in Listeria monocytogenes-infected murine macrophages is distinct from apoptosis. by Barsig (1997)
10.1073/pnas.94.19.10385 / Proc Natl Acad Sci U S A. / Yersinia signals macrophages to undergo apoptosis and YopJ is necessary for this cell death. by Monack (1997)
{'key': '2019111821351165600_B16', 'first-page': '350b', 'article-title': 'Hematopoietic stem cells of CML patients lack monocytic differentiation [abstract].', 'volume': '94', 'author': 'Weissinger', 'year': '1999', 'journal-title': 'Blood.'} / Blood. / Hematopoietic stem cells of CML patients lack monocytic differentiation [abstract]. by Weissinger (1999)
{'key': '2019111821351165600_B17', 'first-page': '323', 'article-title': 'Differential expression of Listeria monocytogenes virulence genes in mammalian host cells.', 'volume': '261', 'author': 'Bubert', 'year': '1999', 'journal-title': 'Mol Gen Gent.'} / Mol Gen Gent. / Differential expression of Listeria monocytogenes virulence genes in mammalian host cells. by Bubert (1999)
10.1073/pnas.91.2.489 / Proc Natl Acad Sci U S A. / A cytolysin encoded by Salmonella is required for survival within macrophages. by Libby (1994)
10.1042/bj1800567 / Biochem J. / Effects of temperature, metabolic inhibitors and some other factors on fluid-phase and adsorptive pinocytosis by rat peritoneal macrophages. by Pratten (1979)
10.1128/IAI.63.11.4268-4276.1995 / Infect Immun. / Listeria monocytogenes infects human endothelial cells by two distinct mechanisms. by Drevets (1995)
10.1016/S0092-8674(00)81070-3 / Cell. / E-cadherin is a receptor for internalin, a surface protein required for entry of Listeria monocytogenes into epithelial cells. by Mengaud (1996)
10.4049/jimmunol.157.2.541 / J Immunol. / Type I (CD64) and type II (CD32) Fc gamma receptor-mediated phagocytosis by human blood dendritic cells. by Fanger (1996)
10.1046/j.1365-2958.1997.1871558.x / Mol Microbiol. / Molecular and chemical characterization of the lipopolysaccharide O-antigen and its role in the virulence of Yersinia enterocolitica serotype O:8. by Zhang (1997)
10.1038/nbt0298-181 / Nat Biotechnol. / Delivery of antigen-encoding plasmid DNA into the cytosol of macrophages by attenuated suicide Listeria monocytogenes. by Dietrich (1998)
10.1016/S0952-7915(96)80083-5 / Curr Opin Immunol. / Recombinant Listeria monocytogenes cancer vaccines. by Paterson (1996)
10.1016/S0092-8674(00)80465-1 / Cell. / Oral somatic transgene vaccination using attenuated Salmonella typhimurium. by Darji (1997)

Dates

Type	When
Created	22 years, 10 months ago (Oct. 30, 2002, 2:43 p.m.)
Deposited	2 years, 4 months ago (April 24, 2023, 3:38 p.m.)
Indexed	1 month, 1 week ago (July 25, 2025, 6:56 a.m.)
Issued	22 years, 9 months ago (Nov. 15, 2002)
Published	22 years, 9 months ago (Nov. 15, 2002)
Published Print	22 years, 9 months ago (Nov. 15, 2002)

Funders 0

None

BibTeX

@article{Kolb_Ma_urer_2002, title={Interaction of human hematopoietic stem cells with bacterial pathogens}, volume={100}, ISSN={0006-4971}, url={http://dx.doi.org/10.1182/blood-2002-03-0898}, DOI={10.1182/blood-2002-03-0898}, number={10}, journal={Blood}, publisher={American Society of Hematology}, author={Kolb-Mäurer, Annette and Wilhelm, Martin and Weissinger, Florian and Bröcker, Eva-Bettina and Goebel, Werner}, year={2002}, month=nov, pages={3703–3709} }

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