DOI: 10.1177/8.4.262. OSMIUM TETROXIDE AND THE MARCHI METHOD: REACTIONS WITH POLAR AND NON-POLAR LIPIDS, PROTEIN AND POLYSACCHARIDE

OSMIUM TETROXIDE AND THE MARCHI METHOD: REACTIONS WITH POLAR AND NON-POLAR LIPIDS, PROTEIN AND POLYSACCHARIDE

10.1177/8.4.262

Crossref journal-article

SAGE Publications

Journal of Histochemistry & Cytochemistry (179)

Abstract

1. The reactions of tissues with OsO4 and the Swank-Davenport KClO3-OsO4 (Marchi) reagent have been studied by blockade of the reducing groups of unsaturated lipid, protein and polysaccharide. Only blockade of the —CH=CH— bond prevented osmiophilia after brief staining (18 hr.). 2. Tissue-protein is neither stained nor demonstrably impregnated after brief treatment with OsO4. 3. Chromatographic separation of degenerating myelin revealed that the Marchi-substance is eluted in the esterified cholesterol fraction. 4. Unsaturated lipid but not protein or polysaccharide are stained by brief treatment with OsO4. The Marchi reaction distinguishes hydrophilic from hydrophobic unsaturated lipid.

Bibliography

ADAMS, C. W. M. (1960). OSMIUM TETROXIDE AND THE MARCHI METHOD: REACTIONS WITH POLAR AND NON-POLAR LIPIDS, PROTEIN AND POLYSACCHARIDE. Journal of Histochemistry & Cytochemistry, 8(4), 262â267.

Authors 1

C. W. M. ADAMS (first)

References 0 Referenced 71

None

Dates

Type	When
Created	14 years, 4 months ago (April 1, 2011, 1:56 a.m.)
Deposited	5 months, 3 weeks ago (March 4, 2025, 8:01 a.m.)
Indexed	5 months, 3 weeks ago (March 4, 2025, 8:40 a.m.)
Issued	65 years, 1 month ago (July 1, 1960)
Published	65 years, 1 month ago (July 1, 1960)
Published Online	65 years, 1 month ago (July 1, 1960)
Published Print	65 years, 1 month ago (July 1, 1960)

Funders 0

None

BibTeX

@article{ADAMS_1960, title={OSMIUM TETROXIDE AND THE MARCHI METHOD: REACTIONS WITH POLAR AND NON-POLAR LIPIDS, PROTEIN AND POLYSACCHARIDE}, volume={8}, ISSN={1551-5044}, url={http://dx.doi.org/10.1177/8.4.262}, DOI={10.1177/8.4.262}, number={4}, journal={Journal of Histochemistry & Cytochemistry}, publisher={SAGE Publications}, author={ADAMS, C. W. M.}, year={1960}, month=jul, pages={262–267} }

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